Cutaneous CD30+ Cells in Children with Atopic Dermatitis

Abstract

Background: CD30 expression can be considered a marker of Th2 cells. We investigated the presence of CD30+ cells in the lesional skin of children with atopic dermatitis (AD). We also analyzed the possible relationship between CD30+ cells and serum soluble CD 30 (sCD30) levels, and IgE, soluble interleukin-2 (IL-2) receptor (sIL-2R) or soluble CD23 (sCD23) levels in the blood, and clinical score. Methods: Ten eczematous children (4 males, 6 females; median age: 4 years and 5 months; range: 11 months to 14 years), 9 sex- and age-matched control children and an adult control group were studied. A clinical score (SCORAD index), was given to eczematous lesions. Blood was taken for the determination of IgE, sCD30, sIL-2R and sCD23 levels. Punch biopsies of lesional skin were stained with hematoxylin and eosin or incubated with anti-CD30 monoclonal antibodies. Skin prick tests (SPTs) were also performed. Results: In the biopsy specimens, CD30 expression was observed in high proportions of infiltrating cells. In children with AD, total serum IgE, sCD30, sIL-2R, sCD23 and eosinophils were significantly elevated compared to controls. CD30+ cells were not associated with serum IgE, sCD30, sIL-2R, sCD23, or SPT results, score of inflammatory cells in lesional skin or clinical score. Children with AD who had high total IgE and specific IgE antibodies did not differ from those with normal total IgE and negative specific IgE in respect of age, clinical score, number of CD30+ cells, sCD30, sIL-2R and sCD23 levels, score of inflammatory cells in skin or clinical score. Conclusion: Our results showed remarkable numbers of CD30+ cells in the lesional skin and high sCD30 in the serum of children with AD. CD30+ cells did not correlate with systemic markers of IgE reaction.

Levels of soluble CD30 in cord blood and peripheral blood during childhood are not correlated with the development of atopic disease or a family history of atopy

“…Furthermore, a similar phenomenon has also been observed with paediatric patients [39–42], although one study reported a failure to associate sCD30 levels in cord blood with atopic disease at the age of 3 years [34]. However, some of these latter studies describe a lack of correlation between sCD30 levels and total serum IgE in children [34, 40, 41]. It is noteworthy that, in contrast to the previous reports with paediatric patients [39–41], we could not observe elevated sCD30 levels at 7 years of age in our children with atopic disease in general or AEDS in particular.…”

“…However, there are certain other differences between these various studies that might explain the different results obtained. In the first place, circulating levels of sCD30 in children and young subjects have been shown to vary with age [37] and, in previous investigations [39–41], the age of the children included in the study groups varied, whereas we determined plasma sCD30 at three specific ages, i.e., at birth and at 2 and 7 years of age. Secondly, the criteria employed for the inclusion of subjects as well as for the evaluation of atopic disease might not have been identical in the different studies.…”

“…Respiratory symptoms in infants can be especially difficult to assess, but in the case of our second study group involving 7‐year‐old children only, this problem was eliminated. Further, the children in our study were recruited to join a prospective study compared with other studies where the patients were recruited on the basis of attending treatment for AEDS [27–29, 39, 41]. The principal association noted in the past has been elevated levels in subjects with AEDS, and it has been observed that the levels decrease with the resolution of AEDS.…”

CD30‐positive lymphoproliferative disorders arising after regional therapy for recurrent melanoma: A report of two cases and analysis of CD30 expression

“…CD30 is expressed in a limited but increasingly reported number of inflammatory dermatoses including atopic dermatitis and arthropod bite reactions . Serum soluble CD30 has been shown to be increased in allergy and asthma in children , as well as in autoimmune disorders such as lupus and rheumatoid arthritis .…”

Histopathological Features in Both the Eschar and Erythematous Lesions of Tsutsugamushi Disease: Identification of CD30+ Cell Infiltration in Tsutsugamushi Disease

“…16,17 It has also been considered a diagnostic feature and a hallmark of cutaneous CD30 lymphoid proliferation (lymphomatoid papulosis/cutaneous CD30 + lymphoma). However, CD30 expression in large lymphoid cells can also be present in the inflammatory infiltrates of benign cutaneous disorders 18 such as atopic dermatitis, 19 drug reactions, 20 scabies, 21 and molluscum contagiosum infection. 22 CD30 expression on T cells has been proposed as a marker for type 2 helper T cells 23 induced by the T H 2 immune response.…”