Cutaneous Human Papillomaviruses as Recurrence Factor in Actinic Keratoses

Dianzani, Caterina; Pierangeli, Alessandra; Chiricozzi, Andrea; Avola, Alessandra; Degener, Anna Marta

doi:10.1177/039463200802100116

Cited by 6 publications

(4 citation statements)

References 28 publications

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“…This low detection rate is contradictory compared with previous studies that reported those of 37% 3 and 77%. 3,4 Differences in conditions of samples, such as formalin-fixed or frozen samples, race and primers may account for the discrepancy in the positive rate between previous studies and ours. HPV type 144 is a gamma virus similar to HPV types 4, 60 and 65 that are typical types of pigmented viral warts.…”

contrasting

confidence: 84%

Detection of human papillomavirus in actinic keratosis

Kuriyama

Shimizu

Ohnishi

et al. 2018

The Journal of Dermatology

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Dear Editor, Bowen's disease, especially in genital and nail lesions and bowenoid papulosis, is recognized as human papillomavirus (HPV)-associated precancerous lesions. 1 While the association between actinic keratosis (AK) and HPV has been rarely reported, some of the beta and gamma HPV types have been reported to increase the risk of squamous cell carcinoma. 2 The aim of this study is to examine whether or not HPV is present in AK using polymerase chain reaction (PCR) and immunohistochemistry.A total of 31 tissue specimens obtained from 30 Japanese patients were examined in this study. Biopsy or surgical specimens were randomly collected from 2006 to 2016. The mean age of patients was 77 years, ranging 56-98. Among 31 AK specimens, 23 were obtained from the face, three from the ear, and one specimen from the neck, finger and forearm, respectively. Formalin-fixed, paraffin-embedded samples were cut into 10-lm sections and DNA was extracted. PCR was performed using L1C1/L1C2 and CPI/CPIIS consensus primers. 1 The amplified PCR products were electrophoresed on 2% agarose gel and the PCR products were sequenced with an automated sequencer. All assays included positive and negative controls. Immunohistochemistry was performed using sections (5-lm thick) which were deparaffinized and rehydrated through a graded series of ethanol. Sections were autoclaved in 5 mmol/L citrate buffer for 10 min at 121°C for antigen retrieval.Human papillomavirus was not detected with the L1C1/ L1C2 primer pair; however, a single band was detected in one specimen using the CPI/CPIIS primer pair (Fig. 1a). With direct sequencing, HPV type 144 was confirmed. Immunohistochemically, the HPV-positive cells were detected in the epidermis adjacent to AK (Fig. 1b), where homogenous cytoplasmic inclusion bodies were observed by hematoxylin-eosin staining (Fig. 1c). All other specimens were negative in immunohistochemistry.In the present study, we examined 31 AK specimens in order to detect HPV by PCR and found that only one specimen was positive for HPV DNA. This low detection rate is contradictory compared with previous studies that reported those of 37% 3 and 77%. 3,4 Differences in conditions of samples, such as formalin-fixed or frozen samples, race and primers may account for the discrepancy in the positive rate between previous studies and ours. HPV type 144 is a gamma virus similar to HPV types 4, 60 and 65 that are typical types of pigmented viral warts. Homogenous intracytoplasmic inclusion bodies, which are characteristic of pigmented viral warts, 5 were seen in the epidermis adjacent to AK in one specimen. We consider (a) (b) (c) Figure 1. Detection of human papillomavirus (HPV) DNA and b-globin using different consensus primer pairs and the histopathology of the HPV type 144-positive tissue. (a) HPV DNA amplification via polymerase chain reaction using CPI/ CPIIS and L1C1/L1C2 primer pairs. b-Globin DNA was amplified using PCO3/PCO4 primers. The first and last lanes indicate HPV type 16 and distilled water which are used as a positiv...

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confidence: 84%

Detection of human papillomavirus in actinic keratosis

Kuriyama

Shimizu

Ohnishi

et al. 2018

The Journal of Dermatology

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“…Neuropeptide SP has been shown to trigger activation of mast cells and results in selective secretion of abundant mediators, such as cytokines and chemokines (40). The mechanism involved may be that SP can directly activate pertussis toxin (PTX)-sensitive G proteins (such as Gi 2 and Gi 3 ) in mast cells, mobilizating phospholipase Cb that causes exocytosis, and stimulating phosphatidylinositol 3-kinase that induces synthesis and release of arachidonic acid metabolites (41).…”

Section: By Opioid Drugsmentioning

confidence: 99%

Agents that induce pseudo-allergic reaction

Wang

Hs²,

Zp³

2011

Drug Discov Ther

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Pseudo-allergic reactions may result from the activation of inflammatory or anaphylactic mechanisms independent of antigen-specific immune responses. Recent statistics show that pseudo-allergic reactions may represent as high as two thirds of all immediate hypersensitivity reactions, implying a great amount of morbidity and numerous health care costs. In this review, we concentrate on agents mediating pseudo-allergic reactions and evaluate accurately the available information on their modes of action. The agents discussed here are divided into three types: (i) Direct mast cell activators, which may activate mast cells in an IgE-independent manner, such as opioid drugs, basic secretagogues and calcium ionophore A23187; (ii) Complement activators, including liposomes, radiocontrast media and Cremophor EL, which may activate the complement system by different pathways: the classical pathway, the mannose-binding lectin pathway or the alternative pathway; (iii) Nonsteroidal anti-inflammatory drugs, which may inhibit the function of cyclooxygenase-1, resulting in the occurrence of adverse reactions. In addition, nonclinical detection methods of pseudoallergic reactions are also reviewed in order to supply valuable information for clinical diagnosis.

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“…In view of the fact that KP is an alteration of the epidermal differentiation which leads to dyskeratosis, in some way similar to psoriasis, an involvement of HPV cannot be excluded. Therefore, a small portion of the biopsy taken from the dorsum, after rubbing the skin with alcohol swabs in order to remove the superficial cells and to eliminate all the possible contaminating factors, was used for HPV testing (10). DNA was extracted from the bioptic sample to search for the presence of HPV; firstly aliquot of the purified DNA was employed in PCR assay to check the suitability of the sample (HLA gene), then it was subjected to different amplifications in order to detect a broad spectrum of HPV types.…”

Section: Case Reportmentioning

confidence: 99%

Ulerythema Ophryogenes, a Rare and Often Misdiagnosed Syndrome: Analysis of an Idiopathic Case

Dianzani

Pizzuti

Gaspardini

et al. 2011

Int J Immunopathol Pharmacol

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Keratosis pilaris (KP) is a follicular hyperkeratosis disorder which is frequently detected in the adult population (44%), mostly in female adolescents (80%). It is a genetic autodominant dermatosis with variable penetrance, but no specific gene association has been determined, even though association to the presence of chromosome 18p deletion has been reported in some cases. We report the case of a 51-year-old Caucasian woman affected by keratosis pilaris gradually progressing with age and with a story of multiple abortions. Standard karyotype and CGH array analyses did not reveale any genetic abnormality. Virological analyses detected the presence of HPV 36 DNA inside the dorsum biopsy, leading to hypothesize its involvement in the evolution of the lesion. Clinical history and patient examination led the diagnosis of an idiopathic case of Ulerythema ophryogenes. The analysis of more cases could be useful to verify the involvement of cutaneous HPV in the progression of the clinical manifestation of the KP variants.

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Cutaneous Human Papillomaviruses as Recurrence Factor in Actinic Keratoses

Cited by 6 publications

References 28 publications

Detection of human papillomavirus in actinic keratosis

Detection of human papillomavirus in actinic keratosis

Agents that induce pseudo-allergic reaction

Ulerythema Ophryogenes, a Rare and Often Misdiagnosed Syndrome: Analysis of an Idiopathic Case

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