Cutaneous leishmaniasis of the new world: Diagnostic immunopathology and antigen pathways in skin and mucosa

Sotto, Mírian Nacagami; Yamashiro-Kanashiro, Edite H.; Matta, Vânia Lúcia Ribeiro da; Brito, Thales de

doi:10.1016/0001-706x(89)90006-5

Cited by 38 publications

(29 citation statements)

References 13 publications

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“…Although vascular and cellular pattern positivity have been previously described (Sells & Burton 1981, Ridley & Ridley 1986, Salinas et al 1989, Sotto et al 1989) their possible value in the diagnosis of the disease was not stressed. In our work the cellular, vascular and neural patterns permitted that immunoperoxidade technique positive results reached from 58.5% (amastigote) to 92.5% in Group I.…”

Section: Discussionmentioning

confidence: 97%

Leishmanial antigens in the diagnosis of active lesions and ancient scars of American tegumentary leishmaniasis patients

Schubach

Cuzzi-Maya

Oliveira

et al. 2001

Mem. Inst. Oswaldo Cruz

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Cutaneous biopsies (n = 94) obtained from 88 patients with American tegumentary leishmaniasis were studied by conventional and immunohistochemical techniques. Specimens were distributed as active lesions of cutaneous leishmaniasis (n = 53) (Group I), cicatricial lesions of cutaneous leishmaniasis (n = 35) (Group II) and suggestive scars of healed mucosal leishmaniasis patients (n = 6) (Group III). In addition, active cutaneous lesions of other etiology (n = 24) (Group C1) and cutaneous scars not related to leishmaniasis (n = 10) (Group C2) were also included in the protocol. Amastigotes in

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Section: Discussionmentioning

confidence: 97%

Leishmanial antigens in the diagnosis of active lesions and ancient scars of American tegumentary leishmaniasis patients

Schubach

Cuzzi-Maya

Oliveira

et al. 2001

Mem. Inst. Oswaldo Cruz

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“…The five LCL and five MCL patients had just started the specific treatment for the disease. The diagnosis of leishmaniasis was confirmed by: the direct parasitological test, histopathological assessment including the demonstration of Leishmania antigen by immunohistochemistry in the lesions 32 , positive MST, and detection of anti-Leishmania antibody in the serum (by indirect immunofluorescence technique and ELISA).…”

Section: Methodsmentioning

confidence: 99%

Cytokine profile in Montenegro skin test of patients with localized cutaneous and mucocutaneous leishmaniasis

Nogueira

Goto

Sotto

et al. 2008

Rev. Inst. Med. trop. S. Paulo

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SuMMarYAmerican tegumentary leishmaniasis presents as two major clinical forms: localized cutaneous leishmaniasis (LCL) and mucocutaneous leishmaniasis (MCL). The immune response in leishmaniasis is efficiently evaluated by the response to Leishmania antigen through the Montenegro skin test (MST). Both LCL and MCL present positive response to MST, indicating that the patients present cell-mediated immunity against the parasite -Leishmania. In spite of the presence of immunity in MCL, this is not sufficient to stop disease progression and prevent resistance to treatment. In this study we demonstrated interleukin (IL) 2, 4, 5 and interferon (IFN) gamma expression in biopsies of MST of ten patients with American tegumentary leishmaniasis. The obtained results were compared between LCL (n = 5) and MCL (n = 5) patients. The MST of MCL patients displayed a higher expression of IL-2, IL-4 and IL-5, in comparison to LCL. There was no significant difference in IFN-gamma expression between groups. The obtained results suggest the role of IL-4 and IL-5 in the maintenance of the immunopathogenic mechanism of the destructive lesions that characterize MCL.

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“…The diagnosis of leishmaniasis was confirmed by positive MST, anti-Leishmania antibody demonstrated by indirect immune fluorescence and/or ELISA assay, and demonstration of parasites in lesions smears or biopsy exam with Leishmania antigen demonstration by immunoperoxidase technique 16 .…”

Section: Methodsmentioning

confidence: 98%

American tegumentary leishmaniasis: langerhans cells in montenegro skin test

Nogueira

Sotto

Cucé

2008

Rev. Inst. Med. trop. S. Paulo

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SuMMarYThis work analyzed the histopathology and epidermal Langerhans cells (LC) of Montenegro skin test (MST) in patients with American tegumentary leishmaniasis (ATL) in order to in situ characterize and compare the immunological reaction of the two major clinical forms of ATL, localized cutaneous leishmaniasis (LCL) and mucocutaneous leishmaniasis (MCL). MST histopathology of both LCL and MCL showed superficial and deep perivascular inflammatory infiltrate composed mainly of lymphocytes and histiocytes. Epidermal LC population was higher in MST biopsies taken from LCL patients when compared to MCL group, at 48 and 72 hours after antigen inoculation. Increased number of epidermal LC displayed in MST biopsies of LCL patients represents specific cellular immunity against parasites. The decrease of LC in MST biopsies of MCL patients does not necessarily indicate a worse specific cellular immunity in this clinical form of leishmaniasis.

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Cutaneous leishmaniasis of the new world: Diagnostic immunopathology and antigen pathways in skin and mucosa

Cited by 38 publications

References 13 publications

Leishmanial antigens in the diagnosis of active lesions and ancient scars of American tegumentary leishmaniasis patients

Leishmanial antigens in the diagnosis of active lesions and ancient scars of American tegumentary leishmaniasis patients

Cytokine profile in Montenegro skin test of patients with localized cutaneous and mucocutaneous leishmaniasis

American tegumentary leishmaniasis: langerhans cells in montenegro skin test

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