Cutaneous &lt;i&gt;Malassezia&lt;/i&gt; Microbiota in Atopic Dermatitis Patients Differ by Gender and Body Part

Akaza, Narifumi; Akamatsu, Hirohiko; Sasaki, Yasuyuki; Takeoka, Shiori; Kishi, Masataka; Mizutani, Hiroshi; Sano, Akiyo; Hirokawa, Keiko; Nakata, Satoshi; Matsunaga, Kayoko

doi:10.1159/000320234

Cited by 15 publications

(14 citation statements)

References 62 publications

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“…However, conventional culture and identification methods offer the advantage of further evaluating the isolates for possible virulence factors, such as the production of phospholipase (44,170) and indole (108,184,336) and melanin synthesis (107). Furthermore, this was highlighted in a study by Akaza et al (6), in which the seasonal rates of isolation of Malassezia species from healthy skin determined by quantitative PCR (qPCR) were compared with those determined by use of Leeming-Notman agar (197). Increased Malassezia colonization of the skin in summer was determined by culture but not by PCR.…”

Section: Resultsmentioning

confidence: 99%

“…Such cycles of population growth, bioactive indole production, and subsequent deprivation of nutrients could result in insufficiently masked antigens and ligands on the surface of the yeast cells, which would result in the activation of the immune system. One study showed that increased numbers of metabolically active cells during summer resulted in higher rates of isolation in culture medium than in fall, although the actual DNA loads were equal in both seasons (6). The difference in the rates of active versus stationary/dead yeasts cells would result in the differential regulation of the skin immune response (316).…”

Section: Seborrheic Dermatitismentioning

confidence: 99%

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The Malassezia Genus in Skin and Systemic Diseases

et al. 2012

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SUMMARY In the last 15 years, the genus Malassezia has been a topic of intense basic research on taxonomy, physiology, biochemistry, ecology, immunology, and metabolomics. Currently, the genus encompasses 14 species. The 1996 revision of the genus resulted in seven accepted taxa: M. furfur , M. pachydermatis , M. sympodialis , M. globosa , M. obtusa , M. restricta , and M. slooffiae. In the last decade, seven new taxa isolated from healthy and lesional human and animal skin have been accepted: M. dermatis , M. japonica , M. yamatoensis , M. nana , M. caprae , M. equina , and M. cuniculi. However, forthcoming multidisciplinary research is expected to show the etiopathological relationships between these new species and skin diseases. Hitherto, basic and clinical research has established etiological links between Malassezia yeasts, pityriasis versicolor, and sepsis of neonates and immunocompromised individuals. Their role in aggravating seborrheic dermatitis, dandruff, folliculitis, and onychomycosis, though often supported by histopathological evidence and favorable antifungal therapeutic outcomes, remains under investigation. A close association between skin and Malassezia IgE binding allergens in atopic eczema has been shown, while laboratory data support a role in psoriasis exacerbations. Finally, metabolomic research resulted in the proposal of a hypothesis on the contribution of Malassezia -synthesized aryl hydrocarbon receptor (AhR) ligands to basal cell carcinoma through UV radiation-induced carcinogenesis.

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Section: Resultsmentioning

confidence: 99%

Section: Seborrheic Dermatitismentioning

confidence: 99%

The Malassezia Genus in Skin and Systemic Diseases

et al. 2012

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“…The skin microbiome in patients with AD was assessed in 11 studies, Table 1 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66. A greater proportion of longitudinal studies and 2 completed randomized controlled trials were performed in AD patients.…”

Section: Resultsmentioning

confidence: 99%

A systematic literature review of the human skin microbiome as biomarker for dermatological drug development

Kolk

Wall

Balmforth

et al. 2018

Brit J Clinical Pharma

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AimsTo explore the potential of the skin microbiome as biomarker in six dermatological conditions: atopic dermatitis (AD), acne vulgaris (AV), psoriasis vulgaris (PV), hidradenitis suppurativa (HS), seborrhoeic dermatitis/pityriasis capitis (SD/PC) and ulcus cruris (UC).MethodsA systematic literature review was conducted according to the PRISMA guidelines. Two investigators independently reviewed the included studies and ranked the suitability microbiome implementation for early phase clinical studies in an adapted GRADE method.ResultsIn total, 841 papers were identified and after screening of titles and abstracts for eligibility we identified 42 manuscripts that could be included in the review. Eleven studies were included for AD, five for AV, 10 for PV, two for HS, four for SD and 10 for UC. For AD and AV, multiple studies report the relationship between the skin microbiome, disease severity and clinical response to treatment. This is currently lacking for the remaining conditions.ConclusionFor two indications – AD and AV – there is preliminary evidence to support implementation of the skin microbiome as biomarkers in early phase clinical trials. For PV, UC, SD and HS there is insufficient evidence from the literature. More microbiome‐directed prospective studies studying the effect of current treatments on the microbiome with special attention for patient meta‐data, sampling methods and analysis methods are needed to draw more substantial conclusions.

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“…The procedure used for the analysis of skin-resident microorganisms was based on our previous report [10]. For the analysis of Propionibacterium spp., 0.05 ml of collected samples and buffer-diluted samples were cultivated on modified GAM agar medium (Nissui Pharmaceutical, Tokyo, Japan) at 35°C for 7 days under anaerobic conditions.…”

Section: Methodsmentioning

confidence: 99%

“…Malassezia spp. were analyzed by real-time PCR using 3 ml of the collected samples in accordance with our previous studies [10,11]. …”

Section: Methodsmentioning

confidence: 99%

Analysis of Facial Skin-Resident Microbiota in Japanese Acne Patients

et al. 2013

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Objectives: We investigated the facial skin microbiota of Japanese acne patients. Methods: Skin swab samples were obtained from 100 acne patients and 28 healthy controls to evaluate Propionibacterium and Staphylococcus spp. using a culture method. Malassezia spp. were evaluated using a nonculture method. Antibiotic resistance of Propionibacterium spp. was also examined. Results: Acne patients and controls did not show significant differences in Propionibacterium and Staphylococcus spp. populations. However, the number of Malassezia globosa from patients was greater than that from controls. Moreover, the number of Propionibacterium spp. from patients carrying antibiotic-resistant strains was significantly greater than that from patients not carrying them. Conclusions: The present study characterized the facial skin microbiota of Japanese acne patients, suggesting a correlation between acne and quantitative differences in Malassezia microbiota. It was also found that the antibiotic resistance of Propionibacterium spp. may affect its abundance in the skin.

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Cutaneous <i>Malassezia</i> Microbiota in Atopic Dermatitis Patients Differ by Gender and Body Part

Cited by 15 publications

References 62 publications

The Malassezia Genus in Skin and Systemic Diseases

The Malassezia Genus in Skin and Systemic Diseases

A systematic literature review of the human skin microbiome as biomarker for dermatological drug development

Analysis of Facial Skin-Resident Microbiota in Japanese Acne Patients

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