Cuticle genes CpCPR63 and CpCPR47 may confer resistance to deltamethrin in Culex pipiens pallens

Sun, Xueli; Guo, Juxin; Ye, Wenyun; Guo, Qin; Huang, Yun; Ma, Lei; Zhou, Dan; Shen, Bo; Sun, Yan; Zhu, Changliang

doi:10.1007/s00436-017-5521-z

Cited by 21 publications

(20 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Adult mosquitoes were reared at 27 °C and 60–70% relative humidity with unlimited access to water and 7% (w/v) sucrose solution under a 16:8 h light:dark photoperiod. To allow females to lay eggs, 3-day-old females were fed fresh mouse blood [18, 19].…”

Section: Methodsmentioning

confidence: 99%

“…Small interfering RNA for silencing the CpCHSB gene (siCHSB) and negative control (NC) RNA was purchased from Gene Pharma (Shanghai, China; Additional file 2: Table S2). Approximately 0.30 μg of siCHSB or NC was injected into the abdomen of third-instar larvae [25] and the thorax of 1-day-old adult female mosquitoes [19], and the same volume of wild type (WT) and negative control (NC) were injected as controls. After 72 h of recovery, knockdown levels of the CpCHSB transcript were measured by RT-qPCR.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Molecular and physiological characterization of the chitin synthase B gene isolated from Culex pipiens pallens (Diptera: Culicidae)

Yang

Yin

et al. 2019

Parasites Vectors

Self Cite

View full text Add to dashboard Cite

BackgroundThe growth and development of insects is strictly dependent on the precise regulation of chitin synthase (CHS), which is absent in vertebrates and plants. Therefore, CHS represents an attractive target for insecticides. At present, the research on the CHS gene in mosquitoes, especially its biological functions, remains limited.ResultsThe full-length cDNA of the chitin synthase B gene in Culex pipiens pallens (CpCHSB) was prepared and consists of 5158 nucleotides with an open reading frame (ORF) of 4722 nucleotides encoding a protein of 1573 amino acid residues. Among different tissues, CpCHSB gene is mainly expressed in the midgut tissue with the highest expression in adult mosquitoes. Knockdown of CpCHSB in the larval stage significantly lowered the chitin content (16.5%) decreased body size (reduced by 25.6% in the larval stage and by 25.6% in the adult stage), and diminished reproduction (20%). Injecting siCHSB into adult mosquito mainly decreased reproduction (27%).ConclusionsCpCHSB plays essential roles in growth and development, by severely reducing larval chitin content, midgut permeability, and reducing the number of female mosquito offspring. These results indicate that CHSB may serve as a potential novel target for exploring biosafe insecticides.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Molecular and physiological characterization of the chitin synthase B gene isolated from Culex pipiens pallens (Diptera: Culicidae)

Yang

Yin

et al. 2019

Parasites Vectors

Self Cite

View full text Add to dashboard Cite

show abstract

“…This could also be because mosquitoes in polluted sites are also frequently exposed to intensive selective pressure induced by pollutants and xenobiotics [27, 50–52], different strategies were reported to promote Culex species adaptation to different ecological constraints. This include the development of resistance or detoxification mechanisms to a large set of insecticides and xenobiotics [53–55], the capacity for eggs to resist desiccation [56] and development of cuticle resistance in larvae [3, 57, 58]. Several Culex species including Cx .…”

Section: Introductionmentioning

confidence: 99%

Culex species diversity, susceptibility to insecticides and role as potential vector of Lymphatic filariasis in the city of Yaoundé, Cameroon

et al. 2019

View full text Add to dashboard Cite

Background Culex species are widespread across Cameroon and responsible for high burden of nuisance in most urban settings. However, despite their high nuisance, they remain less studied compared to anophelines. The present study aimed to assess Culex species distribution, susceptibility to insecticide, bionomics and role in Lymphatic Filariasis (LF) transmission in the city of Yaoundé. Methods Mosquito collections were conducted from March to December 2017 using Centre for Disease Control light traps (CDC-LT), human landing catches (HLC) and larval collections. Mosquitoes were identified using morphological identification keys. Mosquitoes from the Culex pipiens complex were further identified using Polymerase Chain Reaction (PCR) to assess the presence of sibling species. Bioassays were conducted with 2–5 day-old unfed females to assess mosquito susceptibility to DDT, permethrin, deltamethrin and bendiocarb following WHO guidelines. Dead, control and surviving mosquitoes from bioassays were screened by PCR to detect the presence of knockdown resistance ( kdr ) alleles. Pools of mosquitoes were examined by PCR to detect the presence of Wuchereria bancrofti . Results A total of 197,956 mosquitoes belonging to thirteen species were collected. The density of mosquito collected varied according to the collection methods, districts and seasons. Culex quinquefasciatus emerged as the most abundant and the only species of the Culex pipiens complex in Yaoundé. Culex species were found breeding in different types of breeding sites including polluted and unpolluted sites. All Culex species including Cx antennatus , Cx duttoni , Cx perfuscus and Cx tigripes were found to be highly resistant to permethrin, deltamethrin and DDT. Culex quinquefasciatus was also found to be resistant to bendiocarb. A high frequency of the West Africa kdr allele was recorded in resistant Cx . quinquefasciatus . Out of the 247 pooled samples of 25 Culex spp. examined for the presence of Wuchereria bancrofti , none was found infected. Conclusion The study confirms the high adaptation of Culex species particularly Culex quinquefasciatus to the urban environment and no implication of this species in the transmission of LF in Yaoundé Cameroon. Culex species predominance in urban settings highlight potential transmission risk of West Nile and rift valley fever in Yaoundé.

show abstract

“…A previous study in our laboratory investigated expression of the CPR63 gene, encoding an RR-2 family member in Cx. pipiens pallens, and this was more abundant in deltamethrin-resistant (DR) than deltamethrin-susceptible (DS) strains [13]. Furthermore, the mosquito mortality rate was altered by silencing the CPR63 gene [13].…”

Section: Introductionmentioning

confidence: 99%

CPR63 Promotes Pyrethroid Resistance by Increasing Cuticle Thickness in Culex Pipiens Pallens

Zhou

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

The cuticle protein (CP) encoded by CPR63 plays a role in deltamethrin resistance in Culex pipiens pallens. Herein, we investigated the distribution of CPR63 transcripts in this organism, and revealed high expression levels in legs and wings. Furthermore, expression of CPR63 in the legs of deltamethrin-resistant (DR) strains were 1.79-fold higher than in deltamethrin-susceptible (DS) strains. Cuticle analysis of small interfering RNA (siRNA) groups by scanning electron microscopy (SEM) revealed a significantly thinner procuticle of the tarsi in the siCPR63 group than the siNC (negative control (group). Transmission electron microscopy (TEM) revealed that the procuticle, exocuticle and endocuticle thickness of the tarsi were significantly thinner in the siCPR63 group than the siNC group. Our results illuminate the resistance mechanism of CPRs and demonstrate that CPR63 contributes to the resistance phenotype by thickening the cuticle and substantially reducing uptake of insecticides.

show abstract

Cuticle genes CpCPR63 and CpCPR47 may confer resistance to deltamethrin in Culex pipiens pallens

Cited by 21 publications

References 23 publications

Molecular and physiological characterization of the chitin synthase B gene isolated from Culex pipiens pallens (Diptera: Culicidae)

Molecular and physiological characterization of the chitin synthase B gene isolated from Culex pipiens pallens (Diptera: Culicidae)

Culex species diversity, susceptibility to insecticides and role as potential vector of Lymphatic filariasis in the city of Yaoundé, Cameroon

CPR63 Promotes Pyrethroid Resistance by Increasing Cuticle Thickness in Culex Pipiens Pallens

Contact Info

Product

Resources

About