Cuticular Carbohydrates of Three Nematode Species and Chemoreception by Trichostrongylus colubriformis

Bone, Leon W.; Bottjer, Kurt P.

doi:10.2307/3281908

“…Many studies have shown the presence of carbohydrates on GIN surfaces, using labeled lectins (BONE;BOTTJER, 1985;PALMER;McCOMBE, 1996;JURASEK et al, 2010;HILLRICHS et al, 2012). In these studies, Concanavalin A (ConA), Lens culinaris agglutinin (LCA), Maclura pomifera agglutinin (MPA), peanut agglutinin (PNA) and Pisum sativum agglutinin (PSA) were bound to the egg surfaces, suggesting the presence of glucose, mannose and galactose carbohydrates.…”

Section: Introductionmentioning

confidence: 99%

Lectin, hemolysin and protease inhibitors in seed fractions with ovicidal activity against Haemonchus contortus

Salles

¹

,

Braga

²

,

Nascimento³

et al. 2014

Rev. Bras. Parasitol. Vet.

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Bioactive molecules of plant species are promising alternatives for the chemical control of gastrointestinal nematodes in ruminants. Extracts of native and exotic seed species from Brazil's semi-arid region were tested in vitro in an egg hatch assay and the bioactivity of their proteins was investigated. Each seed species was subjected to three extractions with three types of solvents. All the seeds showed ovicidal activity, which varied according to the solvents. Higher ovicidal activity was found in the molecule fractions of low molecular weight (<12 kDa) for Albizia lebbeck, Ipomoea asarifolia, Jatropha curcas, Libidibia ferrea, Moringa oleifera and Ricinus communis (P<0.05, Bonferroni test). The two fractions of Crotalaria spectabilis showed the same ovicidal activity (P>0.05, Bonferroni test). Hemagglutinating activity was detected in the fractions of C. spectabilis and M. oleifera fractions, hemolysin activity in the A. lebbeck and M. oleifera fractions, serine protease inhibitory activity in the A. lebbeck, I. asarifolia, J. curcas, M. oleifera and R. communis fractions, cysteine protease inhibitor activity in the M. oleifera fraction, and no protein activity in the L. ferrea fraction. The results of this work reveal new plant species with a potential for use in controlling nematode parasites in goats, thus opening a new field of research involving plant protein molecules with ovicidal properties.Keywords: Goats, nematodes, egg hatch assay, lectin, protease inhibitors, hemolysin. ResumoMoléculas bioativas de espécies vegetais são alternativas promissoras ao controle químico dos nematoides gastrintestinais em ruminantes. Extratos de sementes de espécies nativas e exóticas do Semiárido Brasileiro foram testados in vitro em ensaio de eclosão de ovos e investigada a natureza proteica da bioatividade. Três extrações com três solventes foram feitas para cada semente estudada. Todas as sementes apresentaram atividade ovicida, variando com o solvente utilizado. Maior taxa de inibição da eclosão concentrou-se nas frações de moléculas de baixa massa molecular (<12 kDa) para Albizia lebbeck, Ipomoea asarifolia, Jatropha curcas, Libidibia ferrea, Moringa oleifera e Ricinus communis (P<0,05, teste de Bonferroni). Crotalaria spectabilis mostrou atividade nas duas frações, sem diferença entre elas (P>0,05, teste de Bonferroni). Observou-se atividade hemaglutinante nas frações de C. spectabilis e M. oleifera, de hemolisina em A. lebbeck e M. oleifera, de atividade inibidora de protease da serina em A. lebbeck, I. asarifolia, J. curcas, M. oleifera

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“…Lectin binding to adult H. contortus has been reported by Bone and Bottjer (1985), but there appear to be no similar studies on T. circumcincta. Some of the 19 lectins used in the present study were also used by Bone and Bottjer (1985), although many lectins were not included in both experiments.…”

Section: Eggsmentioning

confidence: 86%

“…Some of the 19 lectins used in the present study were also used by Bone and Bottjer (1985), although many lectins were not included in both experiments. There was strong binding of some lectins with affinities for GalNAc, Gal and GlcNAc to adult T. circumcincta (Table 2) and also to H. contortus in both studies; however, PTL, which also recognises some α-GalNAc-containing structures, did not bind to either worm.…”

Section: Eggsmentioning

confidence: 99%

“…There was increased binding of some lectins with storage from 2 to 4 months for H. contortus and 3 to 10 months for T. circumcincta, but reduced or less consistent binding with other lectins. (Bone and Bottjer 1985;Hill et al 1991). Changing antigens is considered to be a strategy for evasion of the host immune response (Maizels et al 1993).…”

Section: Exsheathed L3mentioning

confidence: 99%

“…Although not binding to the cuticle of exsheathed L3, lectins do bind to the amphids and phasmids of some parasites (Bowman et al 1988) and may possibly be useful in distinguishing species. In contrast to larvae, many lectins bind strongly to adult nematodes (Bone and Bottjer 1985;Taylor et al 1986) and eggs (Taylor et al 1986;Palmer and McCombe 1996;Colditz et al 2002). H. contortus eggs can be distinguished from those of other species by a positive reaction to peanut agglutinin (PNA) (Palmer and McCombe 1996;Colditz et al 2002;Jurasek et al 2010).…”

Section: Introductionmentioning

confidence: 98%

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Use of fluorescent lectin binding to distinguish Teladorsagia circumcincta and Haemonchus contortus eggs, third-stage larvae and adult worms

Hillrichs

¹

,

Schnieder

²

,

Forbes

³

et al. 2011

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Lectin binding to carbohydrates on parasite surfaces has been investigated as a method of distinguishing adult worms, eggs and sheathed and exsheathed L3 of Teladorsagia circumcincta and Haemonchus contortus, economically important abomasal parasites in temperate climates. Both species were maintained as pure laboratory cultures of field isolates from New Zealand. Each of the four life cycle stages could be distinguished by the binding of at least one lectin: adult worms by Sambucus nigra agglutinin (SNA); eggs by peanut agglutinin (PNA), ConcavalinA and Lens culinaris agglutinin (LCA); exsheathed L3 by Griffonia simplicifolia-I lectin (GSL-I) and Lotus tetragonolobus lectin (LTL) and sheathed L3 by Aleuria aurantia lectin (AAL). The whole surface of both adult T. circumcincta and H. contortus strongly bound lectins specific for N-acetylglucosamine (GlcNAc), N-acetylgalactosamine (GalNAc), mannose and fucose, but the two species could be distinguished by SNA binding only to T. circumcincta. Eggs could be distinguished by the binding of mannose-specific PNA to H. contortus and GalNAc-specific LCA and PSA to T. circumcincta eggs. GalNAc, GlcNAc and mannose lectins bound to the cuticle and over the excretory pores of a large proportion of sheathed L3 of both species, but only the H. contortus surface had exposed fucose or sialic acid complexes. The distinguishing lectin for sheathed L3 was AAL, which did not bind to T. circumcincta, but bound weakly to the head region of all fresh H. contortus and to 50-90% after 3 months storage. The cuticle of exsheathed L3 was unresponsive to all 19 lectins, and any binding was restricted to the head and tail regions. L3 exsheathed after 2-4 months storage could be distinguished by the binding of GSL-I and LTL to H. contortus but not to T. circumcincta. Lectin binding could be a useful adjunct in identifying L3, but lacked the consistency to be definitive, whereas it could be further developed as a practical method of distinguishing parasitic nematodes at other stages in the life cycle, particularly the eggs.

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Invertebrate Endocrinology

Landau

¹

,

Biggers

²

,

Laufer

³

1997

Comprehensive Physiology

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The sections in this article are: Growth and Morphogenetic Factors Insects Crustaceans Molluscs Other Invertebrates Regenerative Factors Crustaceans Insects Cnidarians Platyhelminths Annelids Echinoderms Others Pigmentary Factors Crustaceans Insects Osmoregulatory Factors Factors that May Increase Osmotic Pressure Factors that May Decrease Osmotic Pressure Metabolic Factors Biogenic Amines JHs and Farnesylacetone Adipokinetic Hormones and RPCH Crustacean Hyperglycemic Hormones Other Metabolic Factors Reproductive Factors Flatworms, Nemertines, and Nematodes Molluscs Crustaceans Insects Other Arthropods Annelids Echinoderms Behavioral Factors In Vitro Studies In Vivo Studies Pheromones Summary

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Cuticular Carbohydrates of Three Nematode Species and Chemoreception by Trichostrongylus colubriformis

Cited by 30 publications

References 7 publications

Lectin, hemolysin and protease inhibitors in seed fractions with ovicidal activity against Haemonchus contortus

Lectin, hemolysin and protease inhibitors in seed fractions with ovicidal activity against Haemonchus contortus

Use of fluorescent lectin binding to distinguish Teladorsagia circumcincta and Haemonchus contortus eggs, third-stage larvae and adult worms

Invertebrate Endocrinology

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