The cuticle of Haemonchus contortus, Nippostrongylus brasiliensis and Trichostrongylus colubriformis contained N-acetylgalactosamine and N-acetylglucosamine, based on the binding of fluorescein-labelled lectins. Binding of the lectins varied between the sexes and body regions of the nematodes. Treatment of male T. colubriformis with the lectin Lens culinaris agglutinin (LcA) reduced the feeding by helminths that was stimulated by histamine and the male's response to their female's pheromone, based on in vitro assays. Mannose residues may be involved in the helminth's chemoreceptors for feeding and sexual attraction, based on the specific binding of LcA.
The effect of host serum on in vitro feeding by Trichostrongylus colubriformis was studied by incubating adult helminths in goat serum containing the dye, Rhodamine B. The amount of dye ingested was determined by fluorometric analysis. Immune serum from goats infected with T. colubriformis suppressed helminth feeding, while normal serum from uninfected goats did not. Suppression of feeding by immune serum increased with the duration of the host's infection. Heat-inactivation (56 degrees C) of immune serum did not affect its suppressive activity. Pre-exposure of worms to immune serum decreased subsequent feeding activity. However, rigorous washing of helminths restored their feeding to levels that were similar to untreated worms. Indirect immunofluorescent studies with immune serum and FITC conjugated rabbit anti-goat IgG demonstrated binding of immunoglobulin to the cuticle, stoma and excretory pore of whole worms. Feeding inhibition of immune serum was associated with IgG1 isotype. Results of the present studies indicated that IgG was responsible for in vitro suppression of T. colubriformis feeding and may be one effector of immunity to T. colubriformis in the goat.
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