Effects of host serum on feeding by <i>Trichostrongylus colubriformis</i> (nematoda)

Bottjer, Kurt P.; Klesius, Phillip H.; Bone, Leon W.

doi:10.1111/j.1365-3024.1985.tb00474.x

Cited by 13 publications

(5 citation statements)

References 12 publications

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“…High IgG levels were associated with reduced worm infection in immunized (Harrison et al, 2008) and genetically resistant sheep (Gill, 1991;Pernthaner et al, 2006). The protective activity of IgG was attributed to neutralizing metabolic enzymes (Smith et al, 1985) and suppressing the ability of the worms to feed (Bottjer et al, 1985).…”

Section: Antibody Responsementioning

confidence: 98%

Expression of sheep interleukin 23 (IL23A, alpha subunit p19) in two distinct gastrointestinal diseases

Gossner

Venturina

Peers

et al. 2012

Veterinary Immunology and Immunopathology

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This paper reports the sequence of sheep interleukin 23A (p19), and shows that it shares 98% identity with bovine IL23A, 85% with human and 76% with mouse IL23A. It also reports the existence of two allelic variants that differ largely within the region encoding the amino terminal polypeptide signal sequence. An optimized RT-qPCR assay was used to quantify IL23A transcripts in sheep infected with two common gastrointestinal pathogens, the intracellular bacterium Mycobacterium avium subspecies paratuberculosis and the parasitic nematode Teladorsagia circumcincta. No differential expression of IL23A was detected in the mesenteric lymph node of sheep with the different pathogenic forms of paratuberculosis, however significantly high levels of IL23A were detected in the ileal mucosa of the paucibacillary form in comparison with the asymptomatic or multibacillary forms. Similarly, significantly high levels were present in the gastric lymph node draining Teladorsagia circumcincta-infected abomasum in susceptible sheep. High levels of IL23A seem to be associated with lymphocytic infiltration and inflammation in both diseases but not with the macrophage infiltrate of multibacillary paratuberculosis.

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Section: Antibody Responsementioning

confidence: 98%

Expression of sheep interleukin 23 (IL23A, alpha subunit p19) in two distinct gastrointestinal diseases

Gossner

Venturina

Peers

et al. 2012

Veterinary Immunology and Immunopathology

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“…Furthermore, the smaller size of the worms recovered from resistant sheep ( Figure 2) might suggest that anti-parasite IgA and lgGl antibodies contribute to resistance by neutralizing/inactivating vital metabolic enzymes thus compromising the metabolism, growth, reproductive capacity or mobility of the parasite. Antiparasite IgGl has been shown to suppress Trichostrongylus colubriformis feeding in vitro (Bottjer, Klesius & Bone 1985) and it has been reported that IgA may retard the growth of Ostertugiu in a dose-dependent manner in immune sheep (Smith et al 1985). In addition, IgG antibodies in association with mucus have been shown to significantly reduce the establishment of infective larvae in immune hosts (Carlisle, McGregor & Appelton 1990).…”

Section: Parasite Immunologymentioning

confidence: 99%

Isotype‐specific antibody responses to Haemonchus contortus in genetically resistant sheep

Gill

Gray

Watson

et al. 1993

Parasite Immunology

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The kinetics of anti-Haemonchus antibody responses in serum and faecal extracts of pasture-reared, genetically resistant and random-bred sheep infected with Haemonchus contortus were examined using an isotype-specific ELISA. Anti-Haemonchus antibodies of IgA, IgG1, IgG2 and IgM isotypes were detected in serum and faecal extracts of both resistant and random-bred sheep after challenge infection. Serum IgG1 and IgA levels in resistant sheep were significantly higher than in random-bred sheep between 10 and 31 days after infection. However, there were no differences in IgG2 and IgM antibody responses between the two genotypes. Faecal antibody responses to H. contortus showed a clear genetic effect with resistant sheep exhibiting higher IgA levels throughout infection and higher IgG1 levels between 24 and 31 days after infection. Furthermore, serum IgG1 and IgA, and faecal IgA responses were negatively correlated with faecal egg counts in both genotypes on 17, 24 and 31 days after infection. Together, these results are taken to indicate that anti-parasite IgA and IgG1 antibodies may play an important role in genetically determined resistance of sheep to haemonchosis.

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“…The role of serum and intestinal antibodies in conferring protective immunity against T. colubriformis remains obscure. However, serum from sheep immunised against T. colubriformis has reduced the in vitro feeding and oviposition of the nematode (Bottjer et al 1985;Bone and Klesius 1986). Transfer of passive immunity via immune sheep or guinea-pig serum against T. colubriformis (Connan 1972;Ross and Halliday 1982) or immune sheep serum against T. axei (Ross and Halliday 1979) has also been demonstrated to induce protection against homologous challenge.…”

Section: Discussionmentioning

confidence: 99%

“…Antibodies secreted into the intestine may bind to the nematode molecules (Douch et al 1996) and interfere with nematode feeding (Bottjer et al 1985) and oviposition (Bone and Klesius 1986). For a thorough understanding of the host immune response and the mechanism of parasite survival it would be desirable that initial studies be carried out on suitable laboratory-model animals (Bezubik et al 1988).…”

Section: Introductionmentioning

confidence: 99%

Drug-abbreviated infections of Trichostrongylus colubriformis and development of immunity in jirds (Meriones unguiculatus)

Ziam¹,

Pandey²,

Elegbe³

et al. 2000

Parasitol Res

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The objective of this study was to examine the development and the duration of immunity achieved with drug-abbreviated infections of Trichostrongylus colubriformis in jirds (Meriones unguiculatus). Jirds were primarily infected either by trickle infection with 6 x 100 infective larvae (L3) of T. colubriformis at 3-day intervals or by a single infection with 600 L3. On day 35 post-infection, one batch of jirds from each group was autopsied; the others were treated with oxfendazole at a dose of 5 mg/kg and were challenged with 1,000 L3 on either day 7 or day 42 post-treatment. All jirds were autopsied at 17 days post-challenge. Trickle infection resulted in lower levels of egg production during the primary infection period. The systemic IgM and IgG antibody response was significantly stronger in trickle- and single-infected groups as compared with the negative control group (P < 0.01-P < 0.05). Significantly higher levels of intestinal IgA were demonstrated in trickle- and single-infected groups than in the negative control group (P < 0.01). Numbers of mucosal mast cells increased following infection, but this was not dependent on the type of immunisation. After challenge the extent of worm reduction was greater in trickle-infected than in single-infected subgroups. The IgM and IgG response was significantly stronger in challenged subgroups as compared with negative control subgroups (P < 0.01). However, the IgG response was weaker in control challenged subgroups than in challenged subgroups (P < 0.01). There was a negative correlation between the IgG response and the worm burden after the second challenge (r = -0.73). The acquired immunity to T. colubriformis infection in jirds developed within 5 weeks of primary infection. The level of immunity was higher after trickle infection than after single infection. Furthermore, the immunity persisted for at least 6 weeks after oxfendazole treatment in the absence of a worm burden and larval intake, which is very similar to the situation in domestic ruminant hosts.

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Effects of host serum on feeding by Trichostrongylus colubriformis (nematoda)

Cited by 13 publications

References 12 publications

Expression of sheep interleukin 23 (IL23A, alpha subunit p19) in two distinct gastrointestinal diseases

Expression of sheep interleukin 23 (IL23A, alpha subunit p19) in two distinct gastrointestinal diseases

Isotype‐specific antibody responses to Haemonchus contortus in genetically resistant sheep

Drug-abbreviated infections of Trichostrongylus colubriformis and development of immunity in jirds (Meriones unguiculatus)

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