CXCL12/CXCR4 Mediates Orthodontic Root Resorption via Regulating the M1/M2 Ratio

Fang, Xian; Zhan, Yu; Zhou, Xueman; Wu, Limin; Lin, Junshan; Yi, Yating; Jiang, Chen; Wang, Jun; Liu, J.

doi:10.1177/00220345211050324

Cited by 17 publications

(14 citation statements)

References 37 publications

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“…Compressive force promotes M1 polarization to amplify inflammation. This result is consistent with the reports by He et al [ 9 ], Fang et al [ 27 ], and Zhang et al [ 8 ]. Generally, Arg1 hydrolyzes arginine to ornithine and urea, which characterize M2 polarization and antagonize the metabolic pathway in which iNOS is involved [ 28 ].…”

Section: Discussionsupporting

confidence: 94%

Orthodontic Compression Enhances Macrophage M2 Polarization via Histone H3 Hyperacetylation

Wang

Groeger

Yong

et al. 2023

IJMS

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Orthodontic tooth movement is a complex periodontal remodeling process triggered by compression that involves sterile inflammation and immune responses. Macrophages are mechanically sensitive immune cells, but their role in orthodontic tooth movement is unclear. Here, we hypothesize that orthodontic force can activate macrophages, and their activation may be associated with orthodontic root resorption. After force-loading and/or adiponectin application, the migration function of macrophages was tested via scratch assay, and Nos2, Il1b, Arg1, Il10, ApoE, and Saa3 expression levels were detected using qRT-PCR. Furthermore, H3 histone acetylation was measured using an acetylation detection kit. The specific inhibitor of H3 histone, I-BET762, was deployed to observe its effect on macrophages. In addition, cementoblasts were treated with macrophage-conditioned medium or compression force, and OPG production and cellular migration were measured. We further detected Piezo1 expression in cementoblasts via qRT-PCR and Western-blot, and its effect on the force-induced impairment of cementoblastic functions was also analyzed. Compressive force significantly inhibited macrophage migration. Nos2 was up-regulated 6 h after force-loading. Il1b, Arg1, Il10, Saa3, and ApoE increased after 24 h. Meanwhile, higher H3 histone acetylation was detected in the macrophages subjected to compression, and I-BET762 dampened the expression of M2 polarization markers (Arg1 and Il10). Lastly, even though the activated macrophage-conditioned medium showed no effect on cementoblasts, compressive force directly impaired cementoblastic function by enhancing mechanoreceptor Piezo1. Compressive force activates macrophages; specifically, it causes M2 polarization via H3 histone acetylation in the late stage. Compression-induced orthodontic root resorption is macrophage-independent, but it involves the activation of mechanoreceptor Piezo1.

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Section: Discussionsupporting

confidence: 94%

Orthodontic Compression Enhances Macrophage M2 Polarization via Histone H3 Hyperacetylation

Wang

Groeger

Yong

et al. 2023

IJMS

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“…To explore the influence of the PGO-PHA-AG scaffold on macrophages, RAW 264.7 macrophages were seeded on various scaffolds. iNOS (a marker of M1 macrophage activation) and CD163 (a marker of M2 macrophage polarization) were selected for immunofluorescence staining [ 44 ]. As shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

Polydopamine-mediated graphene oxide and nanohydroxyapatite-incorporated conductive scaffold with an immunomodulatory ability accelerates periodontal bone regeneration in diabetes

Yang

Hou

et al. 2022

Bioactive Materials

128

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“…Human periodontal ligament cells (PDLCs) were obtained from the orthodontically extracted premolars as previously elaborated. 54 Briefly, the middle third of the periodontal ligament was digested with collagenase I (C8140, Solarbio) at 37 °C for 1 h, and then the tissues and cells were cultured with α-MEM medium with 20% fetal bovine serum (Gemini, Australia origin) and 1% penicillin/ streptomycin (P1400, Solarbio). PDLCs used in this research were in passages 4 to 6.…”

Section: Methodsmentioning

confidence: 99%

Effective Orthodontic Tooth Movement via an Occlusion-Activated Electromechanical Synergistic Dental Aligner

Wang,

Zhang,

Yao

et al. 2023

ACS Nano

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Malocclusion is a prevalent dental health problem plaguing over 56% worldwide. Mechanical orthodontic aligners render directional teeth movement extensively used for malocclusion treatment in the clinic, while mechanical regulation inefficiency prolongs the treatment course and induces adverse complications. As a noninvasive physiotherapy, an appropriate electric field plays a vital role in tissue metabolism engineering. Here, we propose an occlusionactivated electromechanical synergistic dental aligner that converts occlusal energy into a piezo-excited alternating electric field for accelerating orthodontic tooth movement. Within an 18-day intervention, significantly facilitated orthodontic results were obtained from young and aged Sprague−Dawley rats, increasing by 34% and 164% in orthodontic efficiency, respectively. The different efficiencies were attributed to age-distributed periodontal tissue status. Mechanistically, the electromechanical synergistic intervention modulated the microenvironment, enhanced osteoblast and osteoclast activity, promoted alveolar bone metabolism, and ultimately accelerated tooth movement. This work holds excellent potential for personalized and effective treatment for malocclusions, which would vastly reduce the suffering of the long orthodontic course.

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CXCL12/CXCR4 Mediates Orthodontic Root Resorption via Regulating the M1/M2 Ratio

Cited by 17 publications

References 37 publications

Orthodontic Compression Enhances Macrophage M2 Polarization via Histone H3 Hyperacetylation

Orthodontic Compression Enhances Macrophage M2 Polarization via Histone H3 Hyperacetylation

Polydopamine-mediated graphene oxide and nanohydroxyapatite-incorporated conductive scaffold with an immunomodulatory ability accelerates periodontal bone regeneration in diabetes

Effective Orthodontic Tooth Movement via an Occlusion-Activated Electromechanical Synergistic Dental Aligner

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