“…Gzma levels were increased, hinting to active NK cells. CT26-HER2 specimens showed an increase also in the cytokines that favor T cell activation ( Cxcl13 , Il2 , Il15 , Ccl5 , Ccl4 ) [ 39 ], in IFN-II and IFN-II-stimulated genes ( Ifng , Cxcl9 , Cxcl10 , Cxcl11 , Gbp2b , Stat1 , Irf1 ), in co-stimulatory genes ( Cd27 , Cd40lg ), in tumor necrosis factors ( Tnf , Fasl , Tnfsf18 , Tnfsf10 ), and in a global Th1-antitumor phenotype ( Tbet , MHC-II ), along with higher expression of inhibitory genes ( Pd1 , Pdl1 , Ctla4 , Lag3 , Ido2 , Havcr2 ). Increased expression of Ifng , Cxcl11 , Tnf , Tbet , and Pdl1 were in agreement with the flow cytometry and RT-PCR results ( Figure 4 I,J,L–N,P).…”