CXCR3 ligands in pleural fluid as markers for the diagnosis of tuberculous pleural effusion

Chung, Wou Young; Jung, Yun Jung; Lee, K; Park, J; Sheen, Seung Soo; Park, K

doi:10.5588/ijtld.17.0232

Cited by 18 publications

(12 citation statements)

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“…The researchers enrolled 336 subjects with PE (106 TPE and 230 non-TPE) and simultaneously evaluated the diagnostic accuracy of ADA, IFN-γ, CXCL9, and CXCL11. They found that these four markers had comparable diagnostic accuracy, with AUCs all higher than 0.95 (68).…”

Section: C-x-c Motif Chemokine Receptor 3 (Cxcr3) Ligandsmentioning

confidence: 99%

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The diagnostic utility of pleural markers for tuberculosis pleural effusion

Zhang¹,

Li²,

Hu³

et al. 2020

Ann Transl Med

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Tuberculosis pleural effusion (TPE) is common in clinical practice, and its diagnosis remains a challenge for clinicians. Ziehl-Neelsen staining, PE Mycobacterium tuberculosis culture, and biopsy are the gold standards for TPE diagnosis; however, they are time-consuming, invasive, observer-dependent, and insensitive. PE markers represent a rapid, low-cost, and non-invasive objective diagnostic tool for TPE. In the past decades, several PE biomarkers have been developed, and their diagnostic accuracy has been evaluated in many studies. Here, we reviewed the literature to summarize the diagnostic accuracy of these biomarkers, especially using the evidence from systematic review and meta-analysis. The current research strongly suggests that adenosine deaminase (ADA), interferon-gamma (IFN-γ), and interleukin 27 (IL-27) have extremely higher diagnostic accuracy for TPE, while the diagnostic accuracy of interferon gamma release assays (IGRAs), tumor necrosis factor-α (TNF-α), and interferon-γ-induced protein 10 kDa (IP-10) is moderate. Although some evidence supports C-X-C motif chemokine ligand 9 (CXCL9), CXCL11, CXCL12, sFas ligand, angiotensin-converting enzyme (ACE), calpain-1, spectrin breakdown products (SBDP), matrix metalloproteinase-1 (MMP-1), soluble CD26 (sCD26), soluble interleukin 2 receptor (sIL-2R) as useful diagnostic markers for TPE, more support is needed to validate their diagnostic accuracy. Finally, nucleic acid amplification tests (NAATs) have extremely high diagnostic specificity, but their sensitivity is low. Taken together, ADA is the preferred marker for TPE because its low cost and suitability for standardization.

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Section: C-x-c Motif Chemokine Receptor 3 (Cxcr3) Ligandsmentioning

confidence: 99%

“…Thus far, only one study has investigated the diagnostic accuracy of CXCL9 and CXCL11 for TPE (68). The researchers enrolled 336 subjects with PE (106 TPE and 230 non-TPE) and simultaneously evaluated the diagnostic accuracy of ADA, IFN-γ, CXCL9, and CXCL11.…”

Section: C-x-c Motif Chemokine Receptor 3 (Cxcr3) Ligandsmentioning

confidence: 99%

The diagnostic utility of pleural markers for tuberculosis pleural effusion

Zhang¹,

Li²,

Hu³

et al. 2020

Ann Transl Med

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“…CXCL11, as a biomarker of TB, has been widely studied. However, for patients with ST, the expression levels of CXCL9 and CXCL10 are poorly understood (11).…”

Section: Introductionmentioning

confidence: 99%

Diagnostic value of CXCR3 and its ligands in spinal tuberculosis

et al. 2020

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The present study aimed to investigate whether C-X-C motif chemokine receptor 3 (CXCR3) and its ligands may aid in diagnosing spinal tuberculosis (ST). A total of 36 patients with ST and 20 healthy controls were enrolled in the present study. The morphology of tuberculous granuloma in spinal tissue was observed by hematoxylin and eosin staining. The presence and distribution of acid-fast bacilli (AFB) were observed by Ziehl-Neelsen (ZN) staining. The protein expression of Ag85B, IFN-γ, and CXCR3 and its ligands (CXCL9 and CXCL10) were detected by immunohistochemistry. The levels of IFN-γ, CXCR3, CXCL9 and CXCL10 in peripheral blood of patients with ST and healthy controls were detected by reverse transcription-quantitative polymerase chain reaction and ELISA. Typical tuberculous granuloma was observed in the ST close tissue. AFB was observed by ZN staining. Positive expression of Ag85B was found in the surrounding caseous necrotic tissue of the tuberculous granuloma. IFN-γ, CXCR3, CXCL9 and CXCL10 were expressed in the tissue surrounding the tuberculous granuloma and their expression levels were markedly higher than those in the distant tissues. The levels of IFN-γ, CXCR3, CXCL9 and CXCL10 in peripheral blood of patients with ST were significantly higher than those in the healthy controls. Receiver operating characteristic curve analysis demonstrated that IFN-γ, CXCR3 and CXCL10 were more reliable diagnostic markers in terms of sensitivity and specificity. IFN-γ, CXCR3, CXCL9 and CXCL10 were highly expressed in the lesion tissue and peripheral blood samples of patients with ST, and IFN-γ, CXCR3 and its ligands aided in diagnosing ST.

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“…Biochemical testing of body uid has been widely performed in the differential diagnosis between transudate and pathological pleural effusions. Though traditional biomarkers, such as ADA, LDH, glucose, protein, have been applied and new markers like IL-27 [29], IFN-γ [30], CXCL9 and CXCL11 [31] are been developing, ADA is still the most cost-effective pleural uid marker. However, its value is questioned in antidiastole and a low prevalence setting.…”

Section: Discussionmentioning

confidence: 99%

Pleural Fluid GSDMD is a Novel Biomarker for the Early Differential Diagnosis of Pleural Effusion

Shi

Zhou

et al. 2020

Preprint

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Introduction: To accurate differential diagnosis of pleural effusion (PE) is still a big challenge. Gasdermin D (GSDMD), controlling pyroptosis in cells, has multiple physiological functions. The diagnostic role of GSDMD in PE remains unknown.Methods: Sandwich ELISA kits that we developed were applied to measure the level of GSDMD for 335 patients with the definite cause of PE, including transudative pleural effusion, tuberculous pleural effusion (TPE), parapneumonic pleural effusion (PPE), and malignant pleural effusion (MPE). Clinical follow up of 40 cases of PPE were conducted and divided into efficacy group and non-efficacy group according to therapeutic outcome. The receiver operating characteristic (ROC) curve was conducted to explore the diagnostic and predictive performance of GSDMD. Nucleated cells (NCs) in pleural effusion were isolated and further infected with bacteria to verify the cell source of GSDMD.Results: In this study, there was prominent statistical significance among the concentration of GSDMD in these four groups (all p < 0.0001, except between MPE and PPE). ROC curve indicated that GSDMD can be an efficient biomarker for differential diagnosis of transudative pleural effusion and other groups (all AUC ＞ 0.973). Noteworthily, the highest AUC belonged to tuberculosis diagnosis of 0.990, and the sensitivity and specificity were 100% and 97.53%. The combination of GSDMD, adenosine deaminase (ADA) and lactate dehydrogenase (LDH) will further improve the diagnostic efficiency especially between TPE and PPE (AUC = 0.968). The AUC of GSDMD change at day 4 could predict the therapeutic effect at an early stage was 0.945 (P < 0.0001). Interestingly, bacterial infection experiments further confirm that the pleural fluid GSDMD was expressed and secreted mainly by the NCs. Conclusion: GSDMD and its combination not only candidate as the potentially novel biomarkers to separate PEs early and effectively, but also monitor disease progression.

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CXCR3 ligands in pleural fluid as markers for the diagnosis of tuberculous pleural effusion

Abstract: Levels of CXR3 ligands in pleural effusion are useful for diagnosing TPE.

Cited by 18 publications

References 0 publications

The diagnostic utility of pleural markers for tuberculosis pleural effusion

The diagnostic utility of pleural markers for tuberculosis pleural effusion

Diagnostic value of CXCR3 and its ligands in spinal tuberculosis

Pleural Fluid GSDMD is a Novel Biomarker for the Early Differential Diagnosis of Pleural Effusion

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CXCR3 ligands in pleural fluid as markers for the diagnosis of tuberculous pleural effusion

Abstract: Levels of CXR3 ligands in pleural effusion are useful for diagnosing TPE.

Cited by 18 publications

References 0 publications

The diagnostic utility of pleural markers for tuberculosis pleural effusion

The diagnostic utility of pleural markers for tuberculosis pleural effusion

Diagnostic value of CXCR3 and its ligands in spinal tuberculosis

Pleural Fluid GSDMD&nbsp;is a Novel Biomarker for the Early Differential Diagnosis of Pleural Effusion

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Pleural Fluid GSDMD is a Novel Biomarker for the Early Differential Diagnosis of Pleural Effusion