Cyanide bioremediation potential of <i>Klebsiella oxytoca</i> JCM 1665 rhodanese immobilized on alginate-glutaraldehyde beads

Itakorode, Babamotemi Oluwasola; Okonji, Raphael Emuebie; Torimiro, Nkem

doi:10.1080/10242422.2022.2087510

Cited by 4 publications

(1 citation statement)

References 55 publications

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“…Since the solution pH affects the chemistry of insoluble support and surface charge of the catalyst, pH is one of the important factors. 76 The influence of pH on the activity of free and immobilized β-agarase was evaluated at various pH values (5.0−10.0). The highest βagarase activity was registered as 100%.…”

Section: Effect Of Ph On the Activity Of Free And Immobilized β-Agarasementioning

confidence: 99%

Enhanced Stability of β-Agarase Immobilized on Streptavidin-Coated Fe₃O₄ Nanoparticles: Effect of Biotin Linker Length

Liu

Bai

et al. 2022

Ind. Eng. Chem. Res.

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This study presents a simple method to immobilize β-agarase onto amino-modified magnetic nanoparticles (MNPs) by biotin/streptavidin (BT/SA) recognition. The effect of BT functionalized with different lengths of poly(ethylene glycol) (PEG) on the activity and stability of heterogeneous biocatalysts was also investigated. The results showed that the enzymatic activity was retained above 93% under the immobilization conditions (pH 7.0, 25 °C, 6 h, and β-agarase concentration of 1 mg/mL). The optimal pH and temperature for both free and immobilized β-agarases were 7 and 30 °C, respectively. PEG length played an important role in the catalytic performance of the immobilized enzyme. As the PEG length increased, an increased trend in activity retention and yield of neoagarobiose was observed. Ultimately, the introduction of PEG 24 improved the activity retention of immobilized β-agarase up to 135.67%, and retained 84.18 and 37.04% of residual activity after incubation for 6 h at 40 and 45 °C, respectively, while only 30.50 and 0% of residual activities were observed for free β-agarase at these temperatures, indicating the significantly enhanced stability of the β-agarase after immobilization. Additionally, β-agarase-BT PEG24 -SA@MNPs showed the highest yield of neoagarobiose at 45 °C, which was 1.89-fold higher than that of the free enzyme. These results indicated that immobilization of β-agarase on BT PEG -SA@MNPs improved both the catalytic efficiency and stability of the enzyme, thereby promoting the continuous production of neoagarobiose.

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Section: Effect Of Ph On the Activity Of Free And Immobilized β-Agarasementioning

confidence: 99%

Enhanced Stability of β-Agarase Immobilized on Streptavidin-Coated Fe₃O₄ Nanoparticles: Effect of Biotin Linker Length

Liu

Bai

et al. 2022

Ind. Eng. Chem. Res.

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Chronic cassava meal modulates body weight, histology and weight of reproductive organs in male albino rats

Egbune,

Orororo

et al. 2023

Toxicol. Environ. Health Sci.

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Characterization of rhodanese synthesized by the wild and EMS-mutated Klebsiella oxytoca JCM1665

Itakorode,

Apalowo,

Boateng

et al. 2024

Journal of Agriculture and Food Research

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Cyanide bioremediation potential of Klebsiella oxytoca JCM 1665 rhodanese immobilized on alginate-glutaraldehyde beads

Cited by 4 publications

References 55 publications

Enhanced Stability of β-Agarase Immobilized on Streptavidin-Coated Fe₃O₄ Nanoparticles: Effect of Biotin Linker Length

Enhanced Stability of β-Agarase Immobilized on Streptavidin-Coated Fe₃O₄ Nanoparticles: Effect of Biotin Linker Length

Chronic cassava meal modulates body weight, histology and weight of reproductive organs in male albino rats

Characterization of rhodanese synthesized by the wild and EMS-mutated Klebsiella oxytoca JCM1665

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Cyanide bioremediation potential of Klebsiella oxytoca JCM 1665 rhodanese immobilized on alginate-glutaraldehyde beads

Cited by 4 publications

References 55 publications

Enhanced Stability of β-Agarase Immobilized on Streptavidin-Coated Fe3O4 Nanoparticles: Effect of Biotin Linker Length

Enhanced Stability of β-Agarase Immobilized on Streptavidin-Coated Fe3O4 Nanoparticles: Effect of Biotin Linker Length

Chronic cassava meal modulates body weight, histology and weight of reproductive organs in male albino rats

Characterization of rhodanese synthesized by the wild and EMS-mutated Klebsiella oxytoca JCM1665

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Product

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Enhanced Stability of β-Agarase Immobilized on Streptavidin-Coated Fe₃O₄ Nanoparticles: Effect of Biotin Linker Length

Enhanced Stability of β-Agarase Immobilized on Streptavidin-Coated Fe₃O₄ Nanoparticles: Effect of Biotin Linker Length