1972
DOI: 10.1016/0003-9861(72)90523-1
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Cyanide degradion by an enzyme from Stemphylium loti

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Cited by 89 publications
(47 citation statements)
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“…It has been suggested that the natural function of acetylene hydratase might be the hydrolysis of toxic substances such as cyanide or nitriles (36). Cyanide hydratases and nitrile hydratases have been described for several aerobic bacteria and fungi (19,32). However, we could demonstrate neither cyanide nor nitrile hydratase activity in cell extracts of P. acetylenicus.…”
Section: Discussioncontrasting
confidence: 78%
“…It has been suggested that the natural function of acetylene hydratase might be the hydrolysis of toxic substances such as cyanide or nitriles (36). Cyanide hydratases and nitrile hydratases have been described for several aerobic bacteria and fungi (19,32). However, we could demonstrate neither cyanide nor nitrile hydratase activity in cell extracts of P. acetylenicus.…”
Section: Discussioncontrasting
confidence: 78%
“…Some species including Stemphylium loti [14], Fusarium lateritium [7], and Gloeocerocospora sorghi [8,17] only detoxify cyanide to formamide by the action of cyanide hydratase and none of these fungi utilizes cyanide as a source of carbon or nitrogen. In contrast, some species such as F. oxysporum, Gliocladium virens, Trichoderma koningii [18], and F. solani IHEM 8026 [9] could grow on KCN as a sole nitrogen source.…”
Section: Discussionmentioning
confidence: 99%
“…Glucose concentration was determined using o-toluidine reagent kit (Sigma, USA). Formamide determination was based on the hydroxamate method [14]. Formate was measured enzymatically with commercial formate dehydrogenase (Sigma, USA) [15].…”
Section: Methodsmentioning
confidence: 99%
“…including Gloeocercospora sorghi (Fry & Munch, 1975 ;Wang et al, 1992), Fusarium lateritium (Cluness et al, 1993) and Stemphylium loti (Fry & Millar, 1972), have also been reported to degrade cyanide. Cyanide hydratases (EC 4.2.1.66) catalysing the hydrolysis of cyanide to formamide have been purified from G. sorghi and F. lateritium, and subsequently characterized.…”
Section: Introductionmentioning
confidence: 99%