Cyanide hydrolysis in a cyanide-degrading bacterium, Pseudomonas stutzeri AK61, by cyanidase

Watanabe, Atsushi; Yano, Kazuyoshi; Ikebukuro, Kazunori; Karube, Isao

doi:10.1099/00221287-144-6-1677

Cited by 84 publications

(67 citation statements)

References 25 publications

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“…Thus, it is not surprising that bacteria have evolved with the capacity to degrade or detoxify HCN. P. stutzeri strain AK61 was isolated from wastewater at a metal-plating plant and was classified phenotypically and chemotaxonomically as a member of P. stutzeri (390). The aim of this study was to develop a biological treatment for cyanide.…”

Section: Biodegradation and Useful Properties For Biotechnological Apmentioning

confidence: 99%

“…Bacterial populations could be useful in detoxifying these agents. Biocides that are degraded and/or resisted by P. stutzeri strains are tributyltin (167,168,299), an organostannic compound used industrially as a stabilizer in plastics and wood preservatives and as an antifouling agent in boat paints; nonoxidizing industrial water treatment bactericides (52)(53)(54)194), used in industrial water cooling systems to avoid microbially induced corrosion of metal surfaces; ␤-cyfluthrin (304), a pesticide used in agriculture to control lepidopteran pests affecting solanaceous crops; and cyanide and thiocyanates (129,174,(389)(390)(391), used in petrochemical refining, the synthesis of organic chemicals and plastics, electroplating, aluminum works, and metal mining.…”

Section: Biodegradation and Useful Properties For Biotechnological Apmentioning

confidence: 99%

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Biology of Pseudomonas stutzeri

Lalucat

Bennasar

Bosch

et al. 2006

Microbiol Mol Biol Rev

552

418

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SUMMARY Pseudomonas stutzeri is a nonfluorescent denitrifying bacterium widely distributed in the environment, and it has also been isolated as an opportunistic pathogen from humans. Over the past 15 years, much progress has been made in elucidating the taxonomy of this diverse taxonomical group, demonstrating the clonality of its populations. The species has received much attention because of its particular metabolic properties: it has been proposed as a model organism for denitrification studies; many strains have natural transformation properties, making it relevant for study of the transfer of genes in the environment; several strains are able to fix dinitrogen; and others participate in the degradation of pollutants or interact with toxic metals. This review considers the history of the discovery, nomenclatural changes, and early studies, together with the relevant biological and ecological properties, of P. stutzeri.

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Section: Biodegradation and Useful Properties For Biotechnological Apmentioning

confidence: 99%

Section: Biodegradation and Useful Properties For Biotechnological Apmentioning

confidence: 99%

Biology of Pseudomonas stutzeri

Lalucat

Bennasar

Bosch

et al. 2006

Microbiol Mol Biol Rev

552

418

View full text Add to dashboard Cite

show abstract

“…Chemical treatment of various cyanide species is a well-established technology for which several commercial processes are available (Carrillo-Pedroza et al 2000). The biological degradation of cyanide compounds has also been extensively demonstrated (Knowles and Bunch 1986;Dorr and Knowles 1989;Meyers et al 1991;Watanabe et al 1998;Dhilon and Shirvaraman 1999).…”

Section: Introductionmentioning

confidence: 99%

Empirical model for the autotrophic biodegradation of thiocyanate in an activated sludge reactor

Plessis

Barnard

Muhlbauer

et al. 2001

Lett Appl Microbiol

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Aims: The aim of this investigation was to develop an empirical model for the autotrophic biodegradation of thiocyanate using an activated sludge reactor. Methods and Results: The methods used for this purpose included the use of a laboratory scale activated sludge reactor unit using thiocyante feed concentrations from 200 to 550 mg l )1 . Reactor ef¯uent concentrations of <1 mg l )1 thiocyanate were consistently achieved for the entire duration of the investigation at a hydraulic retention time of 8 h, solids (biomass) retention of 18 h and biomass (dry weight) concentrations ranging from 2 to 4 g l )1 . A biomass speci®c degradation rate factor was used to relate thiocyanate degradation in the reactor to the prevailing biomass and thiocyanate feed concentrations. A maximum biomass speci®c degradation rate of 16 mg )1 g )1 h )1 (mg thiocyanate consumed per gram biomass per hour) was achieved at a thiocyanate feed concentration of 550 mg l )1 . The overall yield coef®cient was found to be 0á086 (biomass dry weight produced per mass of thiocyanate consumed). Conclusions: Using the results generated by this investigation, an empirical model was developed, based on thiocyanate feed concentration and reactor biomass concentration, to calculate the required absolute hydraulic retention time at which a single-stage continuously stirred tank activated sludge reactor could be operated in order to achieve an ef¯uent concentration of <1 mg l )1 . The use of an empirical model rather than a mechanistic-based kinetic model was proposed due to the low prevailing thiocyanate concentrations in the reactor. Signi®cance and Impact of the Study: These results represent the ®rst empirical model, based on a comprehensive data set, that could be used for the design of thiocyanate-degrading activated sludge systems.

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“…It is frequently referred to as a cyanidase and converts cyanide into ammonia and formate: HCN+ 2H 2 O AE NH 3 + HCOOH (Jandhyala et al, , 2005Watanabe et al, 1998). Structural similarities between these two groups of cyanide-degrading nitrilases and other nitrilases are abundant.…”

mentioning

confidence: 99%

“…2), Bacillus pumilus Meyers et al, 1991Meyers et al, , 1993, Pseudomonas stutzeri AK61 Watanabe et al, 1998) and Alcaligenes xylosoxidans (Banerjee et al, 2006;Ingvorsen et al, 1991). The limited distribution of the enzymes leads to a number of questions.…”

mentioning

confidence: 99%