Cyanidin-3-O-glucoside ameliorates diabetic nephropathy through regulation of glutathione pool

Qin, Yan; Zhai, Qianqian; Li, Yan; Cao, Meng; Xu, Yun; Zhao, Kelei; Wang, Tao

doi:10.1016/j.biopha.2018.04.137

Cited by 52 publications

(30 citation statements)

References 34 publications

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“…On the other hands, Putta et al [ 56 ] and Metwally et al [ 57 ] have suggested that consumption of anthocyanins may lower the risk of diabetes and diabetic complications such as DN. The result of the current study is also in agreement with available literature regarding the presumptive biological activity of each of cyanidin 3‐glucoside [ 34,35,58 ] and cyanidin 3‐rutinoside. [ 59‐61 ]…”

Section: Discussionsupporting

confidence: 91%

“…In a similar study using water extract of green tea as an antioxidant, the diabetic‐treated rats showed amelioration of the histopathological changes like fatty degeneration of renal tubule relative to diabetic rats. [ 63 ] The protective effect of BMFE may be due to its content of antioxidant components such as cyanidin 3‐glucoside [ 33,34,58 ] and cyanidin 3‐rutinoside [ 59‐61 ] that can reduce oxidative stress in the cells, resulting in normal structures and functions. [ 64 ]…”

Section: Discussionmentioning

confidence: 99%

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Black mulberry fruit extract alleviates streptozotocin-induced diabetic nephropathy in rats: targeting TNF-α inflammatory pathway

Abouzed

Sadek

Ghazy

et al. 2020

Journal of Pharmacy and Pharmacology

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Objectives This study was designed to investigate the effect of Morus nigra fruit extract in retarding the progression of diabetic nephropathy in streptozotocin (STZ)-induced diabetic rats. Methods Diabetic male Wistar rats were injected with black mulberry fruit extract (BMFE) at doses of 150 and 300 mg/kg body weight. After 4 weeks, microalbuminuria was estimated in addition to serum concentrations of glucose, insulin, creatinine and albumin. Key findings The study revealed a significant amelioration of all the measured parameters in diabetic animals. In addition, MDA, lipid peroxide levels and catalase activity were also improved. The histopathological examination of kidney tissues revealed significant improvement of the pathological changes and glomerular sclerosis in diabetic rats treated with BMFE. Treated rats showed downregulation of TNF-α, vascular cell adhesion molecule-1 (VCAM-1) and fibronectin mRNA expression. Conclusion The ameliorative effect of BMFE on diabetic nephropathy is not only through its potent antioxidant and hypoglycaemic effects but also through its downregulation of TNF-α, VCAM-1 and fibronectin mRNA expression in renal tissues of diabetic-treated rats. Therefore, BMFE as dietary supplement could be a promising agent in improving diabetic nephropathy. Methods Chemicals Authentic anthocyanin samples of cyanidin-3-O-glucoside and cyanidin-3-O-rutinoside for high-performance liquid chromatography (HPLC) analysis were purchased from Phytoplan company (Germany). Streptozotocin (STZ), chloroform and isopropanol were obtained from Sigma-Aldrich (St. Louis, MO, USA). Authentic samples for HPLC analysis of flavonoids rutin, quercetin, quercetrin, catechin, kaemferol, naringin and phenolic acids chlorogenic, gallic, caffeic, ellagic and cinnamic acids were purchased from Sigma-Aldrich, Germany and were kindly supplied by Food Technology Research Institute, Giza, Egypt. TRizol was obtained from Genetix Branid Co. (Genetix Biotech Asia Pvt. Ltd., New Delhi, India). QuantiFast SYBR Green PCR Master Mix unit was provided by QIAGEN (Hilden, Germany). HiSenScript TM [-] cDNA synthesis kit was purchased from iNtRON Biotechnology Company. The other reagents were of analytical, high-performance liquid chromatography (HPLC), or the best accessible pharmaceutical grades.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Black mulberry fruit extract alleviates streptozotocin-induced diabetic nephropathy in rats: targeting TNF-α inflammatory pathway

Abouzed

Sadek

Ghazy

et al. 2020

Journal of Pharmacy and Pharmacology

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“…However, recent studies have shown that anthocyanins and related derivatives are involved in modulating signaling pathways in mammalian cells 9 . In addition, the effects of anthocyanins on the prevention of cancer and some other chronic illnesses have been demonstrated 10,11 .…”

Section: Introductionmentioning

confidence: 99%

Metabolic and molecular analysis of nonuniform anthocyanin pigmentation in tomato fruit under high light

Zhang

et al. 2019

Hortic Res

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Pigment intensity and patterns are important factors that determine the nutritional and market values of tomato fruits. The acropetal manner of light-dependent anthocyanin accumulation with the highest levels at the stem end of the fruit makes Pro35S:BrTT8 tomato plants an ideal system for investigating the effects of light intensity on anthocyanin biosynthesis. Extensive transcript analyses indicate that anthocyanin pigmentation in Pro35S:BrTT8 plants under high light might be coordinately regulated by the exogenous protein BrTT8 and endogenous proteins SlAN2 and SlMYBL2. Furthermore, yeast two-hybrid assays showed that BrTT8 could interact efficiently with SlAN2, SlMYBL2, and SlAN11. Moreover, the physical interaction between BrTT8 and SlAN2 was validated by FRET. Simultaneous overexpression of SlAN2 and BrTT8 activated significant anthocyanin biosynthesis in infiltrated tobacco leaves. In addition, the ability of SlMYBL2 to suppress anthocyanin accumulation was also demonstrated in infiltrated tobacco leaves. Altogether, these results prove that tissue-specific assemblage of the heterogeneous MYB-bHLH-WD40 complex consisting of SlAN2, BrTT8 and SlAN11 triggers nonuniform anthocyanin accumulation in tomato fruit under high light. Additionally, it is proposed that a negative-feedback loop fulfilled by SlMYBL2 also participates in the regulation of anthocyanin production.

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“…Cy3G, one member of the ACNs family present in various vegetables and fruits, especially edible berries, has been confirmed to have antioxidant properties (38). Cy3G also has been implicated in specific beneficial health actions, including reducing age-associated oxidative stress (39), improving cognitive brain function (40) as well as antidiabetic (41), anti-inflammation (42), and anti-obesity activities (43). Cy3G exhibits anticancer properties in various in vitro and animal models of carcinogenesis and tumor development; however, the mechanisms of those effects established by Cy3G are still elusive.…”

Section: Discussionmentioning

confidence: 99%

Cyanidin-3-O-glucoside inhibits epithelial-to-mesenchymal transition, and migration and invasion of breast cancer cells by upregulating KLF4

Chen

Mei

et al. 2020

Food & Nutrition Research

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Background: Anthocyanins (ACNs) are capable of suppressing breast cancer growth; however, investigation on the effect and mechanism of ACNs on epithelial-to-mesenchymal transition (EMT), and cell migration and invasion in breast cancer cells is limited. A complete understanding of those properties may provide useful information on of how to use these natural compounds for cancer prevention and treatment. Objectives: The aim of this work was to investigate the role of cyanidin-3-O-glucoside (Cy3G), one of the most widely distributed ACNs in edible fruits, in the EMT process, and cell migration and invasion of breast cancer cells, and its underlying molecular mechanisms of how Cy3G establishes these functional roles in these cells. Methods: MDA-MB-231 and MDA-MB-468 breast cancer cells were treated with Cy3G (20 μM) for 24 h, and then the cells were used for cell migration and invasion assay. Western blotting, luciferase assay, ubiquitination assay, gene knockdown, and cycloheximide chase assay were performed to analyze the molecular mechanisms of Cy3G in suppressing EMT, and cell migration and invasion. Results: Cy3G inhibited the EMT process in these cells and significantly suppressed the migration and invasion of breast cancer cells (P ≤ 0.05) by upregulating Krüppel-like factor 4 (KLF4) expression at protein level. KLF4 knockdown in MDA-MB-231 cells did not reveal any change in EMT marker expression, and cell migration and invasion upon treatment with Cy3G (P ≥ 0.05), which strongly indicated that the effects of Cy3G were mediated by KLF4. Furthermore, we determined that Cy3G indirectly upregulated KLF4 expression by downregulating FBXO32, which is the E3 ligase of KLF4. Conclusion: Cy3G is a potential anticancer reagent as it can inhibit EMT and breast cancer cell migration and invasion by upregulating KLF4.

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Cyanidin-3-O-glucoside ameliorates diabetic nephropathy through regulation of glutathione pool

Cited by 52 publications

References 34 publications

Black mulberry fruit extract alleviates streptozotocin-induced diabetic nephropathy in rats: targeting TNF-α inflammatory pathway

Black mulberry fruit extract alleviates streptozotocin-induced diabetic nephropathy in rats: targeting TNF-α inflammatory pathway

Metabolic and molecular analysis of nonuniform anthocyanin pigmentation in tomato fruit under high light

Cyanidin-3-O-glucoside inhibits epithelial-to-mesenchymal transition, and migration and invasion of breast cancer cells by upregulating KLF4

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