Cyanobacteria contain a mitochrondrial complex I‐homologous NADH‐dehydrogenase

Berger, Susanne; Ellersiek, Ulrike; Steinmüller, Klaus

doi:10.1016/0014-5793(91)80957-5

Cited by 87 publications

(54 citation statements)

References 29 publications

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“…PCC 6803, psbG is found in association with genes encoding homologues of two other subunits ofcomplex I [26] (see Fig. 4), and this led to earlier suggestions that psbG was related to an unidentified component of complex I [27,28]. In addition, psbG and ndhJ (the homologue of the 30 kDa subunit of bovine complex I) were detected by immunological means in the same crude enzyme fraction [28].…”

Section: Relationship Of Subunit Psst Lo Proreirt Sequences Ettcoded mentioning

confidence: 87%

“…4), and this led to earlier suggestions that psbG was related to an unidentified component of complex I [27,28]. In addition, psbG and ndhJ (the homologue of the 30 kDa subunit of bovine complex I) were detected by immunological means in the same crude enzyme fraction [28]. Although the suggestion that psbG is a homologue of a complex I subunit has now been proven to be correct, the association of psbG with other genes related to complex I components is rather weak evidence in favour of the proposal.…”

Section: Relationship Of Subunit Psst Lo Proreirt Sequences Ettcoded mentioning

confidence: 95%

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NADH:ubiquinone oxidoreductase from bovine heart mitochondria A fourth nuclear encoded subunit with a homologue encoded in chloroplast genomes

et al. 1992

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The amino acid sequence has been determined of the precursor ofa nuclear encoded 20 kDa subunit ofcomplex I from bovine heart mitochondria. The sequence of the mature protein is related to a protein of uncertain function, hitherto known as psbG, encoded in jhe chloroplast genomes of higher plants. Open reading frames encoding homologues of psbCi have also been detected in bacteria aud in the mitochondrial genomc of Purarn~ci~r~ retmurekt. The chloroplast p&G gcnc is found between n&C and n&J, which encode homologues of ND3. a hydrophobic subunit of complex 1 encoded in the bovine mitochondrial genome, and of the nuclear encoded 30 k'3a subunit of complex 1. This 20 kDa protein is the eleventh out of the forty or more subunits of bovine complex I with a chloroplast encoded homologue. and its sequence provides further support for the prcsencc in chloroplasts of a multisubunit enzyme related to complex I that could be involved in chlororespiration. The strict conservation of threr: cyst&es suggests that the subunit might be an iron-sulphur protein.Complex I (bovine heart mitochondria): Chloroplast genome homologue 1, 1NTRODUCTIONNADH:ubiquinone oxidoreductase (complex I) from bovine heart mitochondria is now known to be a multisubunit assembly of at least 41 different polypeptides [ 121. Seven of them (known as NDI-ND6 and ND4L) are encoded in mitochondrial DNA [3-61, and the remainder are the products of nuclear genes that are imported into the organelle. Homologues of NDl-ND6 and of ND41 were found to be encoded in the chloroplast genomes of higher plants [7-g], and this observation led to the suggestion that chloroplasts contain an enzyme related to complex I, possibly an NAD(P)H:plastoquinone oxidoreductasc involved in a chlororespiratory pathway, although such an enzyme has yet to be purified and characterized. The finding that three nuclear encoded components of mitochondrial complex I have chloroplast encoded homologues [lo-121 provided additional support for the suggestion, and it is endorsed by the results described in the present paper. They show that a nuclear coded 20 kDa component of bovine complex I is related to another protein encoded in chloroplast genomes. This protein, known in the past as psbG, had been thought to be a component of photosystem II [ 131, but this was later shown to be incorrect [14]. It is now much more likely that psbG together with the other chloroplast encoded homologues of complex I is part of a chloroplast multisubunit complex related to complex I. The function of the mitochondrial subunit is not known. Its sequence contains three strictly conserved cysteines which might suggest that it is an iron-sulphur protein. This is consistent with the isolation of the protein from a preparation of a water-soluble fraction of bovine complex I, known as the iron-sulphur protein or IP fraction [ 1 S], and its presence in a sub-complex called Ia, which represents an extensive extramembrane domain containing the NADH binding site, FMN and all of the Fe-S clusters that have been detected by...

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Section: Relationship Of Subunit Psst Lo Proreirt Sequences Ettcoded mentioning

confidence: 87%

Section: Relationship Of Subunit Psst Lo Proreirt Sequences Ettcoded mentioning

confidence: 95%

NADH:ubiquinone oxidoreductase from bovine heart mitochondria A fourth nuclear encoded subunit with a homologue encoded in chloroplast genomes

et al. 1992

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“…The gradient consisted of four layers: 5.5 ml of 58% sucrose, 4.5 ml of 40% sucrose, 4.5 ml of 30% sucrose, and 4 ml of 10% sucrose layer. Thylakoid membranes remained at the 40/58% sucrose interphase, whereas cytoplasmic membranes moved into the 30% sucrose layer that was essentially free of chlorophyll (14).…”

Section: Isolation Of Thylakoid and Cytoplasmic Membranes Frommentioning

confidence: 99%

“…PCC 7942-The above prepared membrane vesicles (total membrane fraction) were used for the separation of thylakoid and cytoplasmic membranes by sucrose gradient centrifugation at 110,000 ϫ g for 16 h (14). The gradient consisted of four layers: 5.5 ml of 58% sucrose, 4.5 ml of 40% sucrose, 4.5 ml of 30% sucrose, and 4 ml of 10% sucrose layer.…”

Section: Isolation Of Thylakoid and Cytoplasmic Membranes Frommentioning

confidence: 99%

Halotolerant Cyanobacterium Aphanothece halophytica Contains an Na+-dependent F1F0-ATP Synthase with a Potential Role in Salt-stress Tolerance

Soontharapirakkul

Promden

Yamada

et al. 2011

Journal of Biological Chemistry

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“…Berger et al (1991) have shown that both purified plasma and thylakoid membranes catalysed rotenone-sensitive NADH oxidation. Furthermore, they showed that antibodies raised against fusion proteins containing the products encoded by ndhK and ndhJ, cross-reacted with proteins on both the plasma and thylakoid membrane, which provides evidence for NDH-1 on both membranes.…”

mentioning

confidence: 99%

Cloning, Analysis and Inactivation of the ndhK Gene Encoding a Subunit of NADH Quinone Oxidoreductase from Anabaena PCC 7120

Howitt

Whelan

Price

et al. 1996

European Journal of Biochemistry

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The function of the type-I pyridine nucleotide dehydrogenase (NDH-1) in the cyanobacterium Anabaena PCC 7120 was investigated. Immunological analysis with antibodies raised against NdhK from Synechocystis PCC 6803, a subunit of NDH-1, showed that NdhK in Anabaena PCC 7120 is only present on the plasma membrane, which confirms the results of previous studies [Howitt, C. A., Smith, G. D. & Day, D. A. (1993) Biochim. Biophys. Acta 1141, 313-3201. Southern analysis with probes from the operon encoding ndhC-K-J from Synechocystis PCC 6803 showed that this operon is also conserved in Anabaena PCC 7120. Part of the operon was amplified using PCR with degenerate primers designed against two sequences encoding regions of NdhC and NdhJ that are conserved between cyanobacteria and chloroplasts. The nucleotide sequence of ndhK encodes a protein of 245 amino acids with a predicted molecular mass of 27.5 kDa. The coding regions of ndhC and ndhK overlap by 7 bp, as found in the chloroplasts of liverwort, maize, and rice. This is markedly different from the case in Synechocystis PCC 6803 where a 71 -bp non-coding, intergenic spacer region lies between ndhC and ndhK. The ndhK clone was interrupted by the insertion of a kanamycin-resistance gene and used to transform Anubaena PCC 7120. 20 unsegregated transformants were produced, all of which died during attempts to segregate them. This indicates that under the selection conditions used, ndhK is an essential gene in Anabaena PCC 7120.

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Cyanobacteria contain a mitochrondrial complex I‐homologous NADH‐dehydrogenase

Cited by 87 publications

References 29 publications

NADH:ubiquinone oxidoreductase from bovine heart mitochondria A fourth nuclear encoded subunit with a homologue encoded in chloroplast genomes

NADH:ubiquinone oxidoreductase from bovine heart mitochondria A fourth nuclear encoded subunit with a homologue encoded in chloroplast genomes

Halotolerant Cyanobacterium Aphanothece halophytica Contains an Na+-dependent F1F0-ATP Synthase with a Potential Role in Salt-stress Tolerance

Cloning, Analysis and Inactivation of the ndhK Gene Encoding a Subunit of NADH Quinone Oxidoreductase from Anabaena PCC 7120

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