Cycas necrotic stunt virus, a new member of nepoviruses found in Cycas revoluta host range, purification, serology and some other properties.

Kusunoki, Makoto; Hanada, Kaoru; Iwaki, Mitsuro; Chang, Moo Ung; Doi, Yoji; Yora, Kiyoshi

doi:10.3186/jjphytopath.52.302

Cited by 15 publications

(10 citation statements)

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“…Cycads exhibit signs of several diseases, including necrotic stunt virus on Cycas revoluta (Kusunoki, 1986), tobacco ringspot virus on Zamia furfuracea (Baker, 2007), leaf-spot and leaf-freckle damage on several species (Tandon and Bilgrami, 1957), as well as other occurrences involving single species (Dodger, 1972;Jian et al, 2006). By contrast, the more intensively examined G. biloba, the remaining extant species of a vast ginkgophyte clade dominant during the Mesozoic, possesses numerous known diseases (Aoki, 1997).…”

Section: Can Plant Diseases Be Recognized In the Preangiospermous Fosmentioning

confidence: 98%

Plant paleopathology and the roles of pathogens and insects

Labandeira

Prevec

2014

International Journal of Paleopathology

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Section: Can Plant Diseases Be Recognized In the Preangiospermous Fosmentioning

confidence: 98%

Plant paleopathology and the roles of pathogens and insects

Labandeira

Prevec

2014

International Journal of Paleopathology

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“…Cycas necrotic stunt virus , a member of the genus Nepovirus , family Secoviridae , was first identified in the gymnosperm Cycas revoluta in Japan ( Kusunoki et al, 1986 ; Han et al, 2002 ). The severely affected plants deteriorated and subsequently died ( Kusunoki et al, 1986 ). Cycas necrotic stunt virus (CNSV) and similar viruses have also been isolated from gladiolus ( Gladiolus spp.…”

Section: Alfalfa Viruses Found In Public Repositoriesmentioning

confidence: 99%

“…), peony ( Paeonia lactiflora Pall. ), Easter lily ( Lilium longiflorum ), aucuba ( Aucuba japonica ), daphne ( Daphne odora ) and spring onions ( Allium fistlosum ) ( Kusunoki et al, 1986 ; Han et al, 2002 ; Ochoa-Corona et al, 2003 ; Hanada et al, 2006 ; Wylie et al, 2012 ; Lim et al, 2019 ; Shaffer et al, 2019 ). Until recently, CNSV had not been found in alfalfa.…”

Section: Alfalfa Viruses Found In Public Repositoriesmentioning

confidence: 99%

High-Throughput Sequencing for Deciphering the Virome of Alfalfa (Medicago sativa L.)

2020

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Alfalfa ( Medicago sativa L.), also known as lucerne, is a major forage crop worldwide. In the United States, it has recently become the third most valuable field crop, with an estimated value of over $9.3 billion. Alfalfa is naturally infected by many different pathogens, including viruses, obligate parasites that reproduce only inside living host cells. Traditionally, viral infections of alfalfa have been considered by breeders, growers, producers and researchers to be diseases of limited importance, although they are widespread in all major cultivation areas. However, over the past few years, due to the rapid development of high-throughput sequencing (HTS), viral metagenomics, bioinformatics tools for interpreting massive amounts of HTS data and the increasing accessibility of public data repositories for transcriptomic discoveries, several emerging viruses of alfalfa with the potential to cause serious yield losses have been described. They include alfalfa leaf curl virus (family Geminiviridae ), alfalfa dwarf virus (family Rhabdoviridae ), alfalfa enamovirus 1 (family Luteoviridae ), alfalfa virus S (family Alphaflexiviridae ) and others. These discoveries have called into question the assumed low economic impact of viral diseases in alfalfa and further suggested their possible contribution to the severity of complex infections involving multiple pathogens. In this review, we will focus on viruses of alfalfa recently described in different laboratories on the basis of the above research methodologies.

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“…So far, viruses similar to CNSV have been isolated in Chiba and Ibaraki Prefectures. They were also isolated from aucuba (Aucuba japonica), daphne (Daphne odora), and allium (Allium fistlosum), as cited in Kusunoki et al (1986). We have started to determine the sequence of the CP gene of CNSV isolated from aucuba.…”

mentioning

confidence: 99%

“…Antiserum already prepared against CNSV-C described by Kusunoki et al (1986) was used. Antisera prepared against Arabis mosaic virus (ArMV), Tomato black ring virus (TBRV), and Tomato ringspot virus (ToRSV) classified into nepoviruses in Japan were also used (Iwaki and Komuro 1971, 1973, 1974Fukumoto et al 1982).…”

mentioning

confidence: 99%

Cycas necrotic stunt virus isolated from gladiolus plants in Japan

et al. 2006

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An undescribed spherical virus ca. 30 nm in diameter was isolated from gladiolus (Gladiolus spp.) plants in Japan. The virus had a moderate host range within eight families. Purified virus preparations contained two large RNA components and one coat protein with mobility similar to Cycas necrotic stunt virus (CNSV) from cycas (Cycas revolute). The virus was serologically closely related to CNSV. Its nucleotide sequence of the coat protein gene had 89% common identity with that of CNSV. These results indicated that the virus isolated from gladiolus is a new strain of CNSV. Key words Cycas necrotic stunt virus · Nepovirus · GladiolusIn Japan, four distinct viruses, Bean yellow mosaic virus, Cucumber mosaic virus, Tobacco mosaic virus, and Tobacco ringspot virus, were isolated from gladiolus (Gladiolus spp.) plants with virus-like symptoms as reported by Fukumoto et al. (1982). An unidentified virus with small spherical particles was also found in three samples (cvs.Topaz and Purple-Supreme) among these gladiolus plants.In this article we describe some properties of the spherical virus and the identification of the virus.A spherical virus was detected from 3 gladiolus plants (1 plant of cv. Topaz and two of Purple-Supreme) out of 72 plants with virus-like symptoms collected in Japan (Fukumoto et al. 1982). The three virus isolates retained similar properties after brief characterization, and an isolate from Purple-Supreme in Ibaraki Prefecture was selected as the type isolate for further characterization. Leaf extract prepared from leaves on the gladiolus by grinding with 0.05 M neutral phosphate buffer was first used to mechanically inoculate Chenopodium quinoa, then inoculum prepared from subsequently systemically infected C. quinoa leaves was used for subculturing the virus for characterization. Infected C. quinoa leaves contained ca. 30-nm diameter, spherical virus-like particles as seen with electron microscopy. The virus was registered with Genebank of MAFFIN as no. 307036.To determine the host range of the virus, we used sap for mechanical inoculation of plants in 32 species from ten families. All plants were grown in a glasshouse. Symptoms were monitored for at least 1 month after inoculation. As shown in Table 1, obvious systemic symptoms appeared on Glycine max (soybean), Arachis hypogaea (peanut), Spinacia oleracea (spinach), Gomphrena globosa (globe amaranth), Zinnia elegans (zinnia), C. quinoa, Chenopodium amaranticolor, and some other plants. Local necrotic lesions were present on inoculated leaves of Phaseolus vulgaris (kidney bean), Petunia hybrida (petunia), and Sesamum indicum (sesame). When symptoms were not obvious, plants of C. amaranticolor or C. quinoa were backinoculated to check infection. In total, plants of 22 species in ten families were infected by the virus (Table 1), giving the virus a moderately wide host range.As described, the virus had spherical particles (ca. 30 nm in diameter) and infected Chenopodium spp. plants, producing a prominent mottle. Preliminary results using partially ...

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Cycas necrotic stunt virus, a new member of nepoviruses found in Cycas revoluta host range, purification, serology and some other properties.

Cited by 15 publications

References 1 publication

Plant paleopathology and the roles of pathogens and insects

Plant paleopathology and the roles of pathogens and insects

High-Throughput Sequencing for Deciphering the Virome of Alfalfa (Medicago sativa L.)

Cycas necrotic stunt virus isolated from gladiolus plants in Japan

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