Cycle of the seminiferous epithelium in a marsupial species, the brushtail possum (Trichosurus vulpecula), and estimation of its duration

Lin, Mingwei; Harman, Amanda; Fletcher, Timothy David

doi:10.1071/rd02059

Cited by 10 publications

(12 citation statements)

References 26 publications

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“…Possum fibrous sheath morphogenesis is described using the ten stages of the seminiferous epithelium cycle and the 14 steps of spermatid development as described by Lin et al. (2004).…”

Section: Resultsmentioning

confidence: 99%

“…(1990)]. In the possum, peak cytoplasmic immunoreactivity occurs in step 10 spermatids, well after chromatin condensation has become initiated (Lin et al. 2004).…”

Section: Discussionmentioning

confidence: 99%

“…Although several ultrastructural studies of possum spermiogenesis have already been carried out (Harding et al. 1976; Lin et al. 2004), little data are available on sperm tail morphogenesis.…”

Section: Introductionmentioning

confidence: 99%

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Morphogenesis of the fibrous sheath in the marsupial spermatozoon

Ricci

Breed

2005

Journal of Anatomy

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The spermatozoon fibrous sheath contains longitudinal columns and circumferential ribs. It surrounds the axoneme of the principal piece of the mammalian sperm tail, and may be important in sperm stability and motility. Here we describe its assembly during spermiogenesis in a marsupial, the brush-tail possum, and compare its structural organization with that of eutherian mammals, birds and reptiles. Transmission electron microscopy showed that possum fibrous sheath assembly is a multistep process extending in a distal-to-proximal direction along the axoneme from steps 4 to 14 of spermiogenesis. For the most part, assembly of the longitudinal columns occurs before that of the circumferential ribs. Immunohistochemical and immunogold labelling showed that fibrous sheath proteins are first present in the spermatid cytoplasm; at least some of the proteins of the sheath precursors differ from those in the mature fibrous sheath. That immunoreactivity develops after initiation of chromatin condensation suggests that fibrous sheath proteins, or their mRNAs, are stored within the spermatid cytoplasmic lobule prior to their assembly along the axoneme.These findings are similar to those in laboratory rats, and thus suggests that the mode of fibrous sheath assembly evolved in a common ancestor over 125 million years ago, prior to the divergence of marsupial and eutherian lineages.

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“…Possum fibrous sheath morphogenesis is described using the ten stages of the seminiferous epithelium cycle and the 14 steps of spermatid development as described by Lin et al. (2004).…”

Section: Resultsmentioning

confidence: 99%

“…(1990)]. In the possum, peak cytoplasmic immunoreactivity occurs in step 10 spermatids, well after chromatin condensation has become initiated (Lin et al. 2004).…”

Section: Discussionmentioning

confidence: 99%

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Morphogenesis of the fibrous sheath in the marsupial spermatozoon

Ricci

Breed

2005

Journal of Anatomy

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“…The seminiferous epithelial cycle and micro-anatomy of the CW (MacCallum 2004) and SHNW (Oishi et al 2013) testis have been described. Both species have similar seminiferous tubule mean diameters (CW: 234.0G4.6 mm, SHNW: 243.5G3.9 mm), possess a high proportion of pre-meiotic (CW: 61.7%, SHNW: 66.6%) compared with post-meiotic stages of the seminiferous cycle and have a frequency of cellular associations and dynamics similar to those in other marsupials (Setchell & Carrick 1973, Lin et al 2004, Phillips et al 2008. Interestingly, round spermatid nuclei in vombatids (i.e.…”

Section: Male Micro-anatomymentioning

confidence: 93%

Wombat reproduction (Marsupialia; Vombatidae): an update and future directions for the development of artificial breeding technology

Hogan¹,

Janssen²,

Johnston³

2013

Reproduction

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This review provides an update on what is currently known about wombat reproductive biology and reports on attempts made to manipulate and/or enhance wombat reproduction as part of the development of artificial reproductive technology (ART) in this taxon. Over the last decade, the logistical difficulties associated with monitoring a nocturnal and semi-fossorial species have largely been overcome, enabling new features of wombat physiology and behaviour to be elucidated. Despite this progress, captive propagation rates are still poor and there are areas of wombat reproductive biology that still require attention, e.g. further characterisation of the oestrous cycle and oestrus. Numerous advances in the use of ART have also been recently developed in the Vombatidae but despite this research, practical methods of manipulating wombat reproduction for the purposes of obtaining research material or for artificial breeding are not yet available. Improvement of the propagation, genetic diversity and management of wombat populations requires a thorough understanding of Vombatidae reproduction. While semen collection and cryopreservation in wombats is fairly straightforward there is currently an inability to detect, induce or synchronise oestrus/ovulation and this is an impeding progress in the development of artificial insemination in this taxon.Reproduction (2013) 145 R157-R173

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“…) and other marsupials (Mason & Blackshaw, ; Setchell & Carrick, ; Orsi & Ferreira, ; Queiroz & Nogueira, ; Lin et al. ; Lloyd et al. ; Phillips et al.…”

Section: Methodsmentioning

confidence: 99%

The seminiferous epithelial cycle and microanatomy of the koala (Phascolarctos cinereus) and southern hairy‐nosed wombat (Lasiorhinus latifrons) testis

et al. 2013

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The koala (Phascolarctos cinereus) and southern hairy-nosed wombat (Lasiorhinus latifrons) are iconic Australian fauna that share a close phylogenetic relationship but there are currently no comparative studies of the seminiferous epithelial cell or testicular microanatomy of either species. Koala and wombat spermatozoa are unusual for marsupials as they possess a curved stream-lined head and lateral neck insertion that superficially is similar to murid spermatozoa; the koala also contains Sertoli cells with crystalloid inclusions that closely resemble the Charcot-Bottcher crystalloids described in human Sertoli cells. Eighteen sexually mature koalas and four sexually mature southern hairy-nosed (SHN) wombats were examined to establish base-line data on quantitative testicular histology. Dynamics of the seminiferous epithelial cycle in the both species consisted of eight stages of cellular association similar to that described in other marsupials. Both species possessed a high proportion of the pre-meiotic (stages VIII, I -III; koala -62.2 AE 1.7% and SHN wombat -66.6 AE 2.4%) when compared with post-meiotic stages of the seminiferous cycle. The mean diameters of the seminiferous tubules found in the koalas and the SHN wombats were 227.8 AE 6.1 and 243.5 AE 3.9 lm, respectively. There were differences in testicular histology between the species including the koala possessing (i) a greater proportion of Leydig cells, (ii) larger Sertoli cell nuclei, (iii) crystalloids in the Sertoli cell cytoplasm, (iv) a distinctive acrosomal granule during spermiogenesis and (v) a highly eosinophilic acrosome. An understanding of the seminiferous epithelial cycle and microanatomy of testis is fundamental for documenting normal spermatogenesis and testicular architecture; recent evidence of orchitis and epididymitis associated with natural chlamydial infection in the koala suggest that this species might be useful as an experimental model for understanding Chlamydia induced testicular pathology in humans. Comparative spermatogenic data of closely related species can also potentially reflect evolutionary divergence and differences in reproductive strategies.

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Cycle of the seminiferous epithelium in a marsupial species, the brushtail possum (Trichosurus vulpecula), and estimation of its duration

Cited by 10 publications

References 26 publications

Morphogenesis of the fibrous sheath in the marsupial spermatozoon

Morphogenesis of the fibrous sheath in the marsupial spermatozoon

Wombat reproduction (Marsupialia; Vombatidae): an update and future directions for the development of artificial breeding technology

The seminiferous epithelial cycle and microanatomy of the koala (Phascolarctos cinereus) and southern hairy‐nosed wombat (Lasiorhinus latifrons) testis

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