Cycles of heat and aerial-exposure induce changes in the transcriptome related to cell regulation and metabolism in Mytilus californianus

Connor, Kwasi M.; Gracey, Andrew Y.

doi:10.1007/s00227-020-03750-6

Cited by 26 publications

(24 citation statements)

References 52 publications

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“…However, despite the general common daily pattern for this function, a clear phase shift is noted between stations, with peaks of expression between 22h and 7h at South ( Figure 3 C), and between 10h and 23h at North ( Figure 3 E), highlighting again a clear environment-specific modulation of cellular processes. While chronobiological data are still scarce in marine organisms, some DNA repair genes have been identified to peak during nighttime in the mussel Mytilus californiaunus under temperate region’s natural environment simulation in the laboratory ( Connor and Gracey, 2020 ). Another function strongly represented by rhythmic transcripts is “protein phosphorylation and ubiquitination”.…”

Section: Resultsmentioning

confidence: 99%

Widely rhythmic transcriptome in Calanus finmarchicus during the high Arctic summer solstice period

et al. 2021

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Summary Solar light/dark cycles and seasonal photoperiods underpin daily and annual rhythms of life on Earth. Yet, the Arctic is characterized by several months of permanent illumination (“midnight sun”). To determine the persistence of 24h rhythms during the midnight sun, we investigated transcriptomic dynamics in the copepod Calanus finmarchicus during the summer solstice period in the Arctic, with the lowest diel oscillation and the highest altitude of the sun's position. Here we reveal that in these extreme photic conditions, a widely rhythmic daily transcriptome exists, showing that very weak solar cues are sufficient to entrain organisms. Furthermore, at extremely high latitudes and under sea-ice, gene oscillations become re-organized to include <24h rhythms. Environmental synchronization may therefore be modulated to include non-photic signals (i.e. tidal cycles). The ability of zooplankton to be synchronized by extremely weak diel and potentially tidal cycles, may confer an adaptive temporal reorganization of biological processes at high latitudes.

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Section: Resultsmentioning

confidence: 99%

Widely rhythmic transcriptome in Calanus finmarchicus during the high Arctic summer solstice period

et al. 2021

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“…We assessed whether microplastics caused protein denaturing in gill tissue. Chaperone gene Hsp70B2 has been shown to respond to toxins and heat in previous studies of Mytilus californianus [28][29][30][31]. We first performed a proof-of-concept experiment to show that Hsp70B2 responds to heat stress in M. galloprovincialis.…”

Section: Gene Expressionmentioning

confidence: 99%

The observation of starch digestion in blue mussel Mytilus galloprovincialis exposed to microplastic particles under varied food conditions

et al. 2021

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Microplastic continues to be an environmental concern, especially for filter feeding bivalves known to ingest these particles. It is important to understand the effects of microplastic particles on the physiological performance of these bivalves and many studies have investigated their impact on various physiological processes. This study investigated the effects of microplastic (10 μm) on digestive enzyme (amylase) activity of Mytilus galloprovincialis at 55,000 and 110,000 microplastic particles/L under laboratory conditions. Additionally, our study measured the expression of an isoform of Hsp70 in the gills to assess whether or not these particles may cause protein denaturation. Results revealed that this regime negatively affect the ability of M. galloprovincialis to digest starch under high food conditions but not low food conditions. Exposure to extreme levels of microplastic raised amylase activity. Furthermore, Hsp70 transcript abundance was not elevated in treatment mussels. These results show that mussels may be resilient to current microplastic pollution levels in nature.

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“…The circadian oxidative stress proteins in our study may show more of a response to a feeding and activity regime than the tidal emersion conditions we imposed. Given the lack of anaerobic metabolism proteins, it is possible that the predictable tidal emersion failed to impose hypoxia significant enough to elicit a prominent stress response (Connor & Gracey, 2020; Widdows & Shick, 1985). However, coordinated peaks appeared for a subset of TCA cycle proteins preceding the formation of the anabolic reducing equivalent NADPH by NADP + ‐dependent isocitrate dehydrogenase.…”

Section: Resultsmentioning

confidence: 99%

Circadian and circatidal rhythms of protein abundance in the California mussel (Mytilus californianus)

Elowe

Tomanek

2021

Molecular Ecology

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Coastal habitats fluctuate with the 12.4 h tidal and 24 h light/dark cycle to predictably alter conditions such as air exposure, temperature, and food availability. Intertidal sessile bivalves exhibit behavioural and physiological adjustments to minimize the challenges of this environment. We investigated a high‐resolution time course of the changes in protein abundance in the gill tissue of the intertidal mussel Mytilus californianus in a simulated tidal environment of 12:12 h light:dark cycles and a matching 6:6 h high:low tide cycle within each 12 h period. Approximately 38% of detected proteins showed significant rhythms in their abundances, with diversity in the phases of rhythmic isoforms. The circadian rhythm was dominant in protein abundance changes, particularly with oxidative metabolism. A tidal cycle elicited changes within functional groups, including in cytoskeletal proteins, chaperones, and oxidative stress proteins. In addition to protein abundance changes, we found the possibility for post‐translational modifications driving rhythms, including methylation, mitochondrial peptide processing (proteolysis), and acylation. Dynamic changes in the proteome across functional categories demonstrate the importance of the tidal environment in entraining cellular processes, confirming that differential expression studies should not assume a static baseline of cellular conditions in intertidal organisms.

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Cycles of heat and aerial-exposure induce changes in the transcriptome related to cell regulation and metabolism in Mytilus californianus

Cited by 26 publications

References 52 publications

Widely rhythmic transcriptome in Calanus finmarchicus during the high Arctic summer solstice period

Widely rhythmic transcriptome in Calanus finmarchicus during the high Arctic summer solstice period

The observation of starch digestion in blue mussel Mytilus galloprovincialis exposed to microplastic particles under varied food conditions

Circadian and circatidal rhythms of protein abundance in the California mussel (Mytilus californianus)

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