Cyclic adenosine 3′,5′-monophosphate as “first messenger” inStreptomyces hygroscopicus—Bimodal regulation of germination and growth

Gersch, D.; Strunk, Christa

doi:10.1007/bf02602830

Cited by 9 publications

(2 citation statements)

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“…However, in the gram-positive bacteria, the function (and even the existence) of cAMP is unsure (34,37,48). In the actinomycetes, cAMP appears to play a role in differentiation (14), antibiotic production (35), and glyoxylate shunt regulation (45), but correlations of cAMP levels with biosynthesis rates of extracellular enzymes have not been published. In a preliminary study (11), cAMP pool levels in a cellulase-hyperproducing mutant of the thermophilic actinomycete Thermomonospora curvata were severalfold those of the wild type.…”

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confidence: 99%

Cyclic AMP levels during induction and repression of cellulase biosynthesis in Thermomonospora curvata

Wg¹,

Neubauer²,

Stutzenberger³

1984

J Bacteriol

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Specific cellulase production rates (SCPR) were compared with intracellular cyclic AMP (cAMP) levels in the thermophilic actinomycete, Thermomonospora curvata, during growth on several carbon sources in a chemically defined medium. SCPR and cAMP levels were 0.03 U (endoglucanase [EG] units) and 2 pmol per mg of dry cells, respectively, during exponential growth on glucose. These values increased to about 6 and 25, respectively, during growth on cellulose. Detectable EG production ceased when cAMP levels dropped below 10. Cellobiose (usually considered to be a cellulase inducer) caused a sharp decrease in cAMP levels and repressed EG production when added to cellulose-grown cultures. 2-deoxy-D-glucose, although nonmetabolizable in T. curvata, depressed cAMP to levels observed with glucose, but unlike glucose, the 2DG effect persisted until cells were washed and transferred to fresh medium. SCPR values and cAMP levels in cells grown in continuous culture under conditions of cellobiose limitation were markedly influenced by dilution rate (D). The maxima for both occurred at D = 0.085 (culture generation time of 11.8 h). When D was held constant and cellobiose concentration was increased over a 14-fold range to support higher steady state population levels, SCPR values decreased about fivefold, indicating that extracellular catabolite accumulation may be a factor in EG repression. The role of cAMP in the mechanism of this repression appears to be neither simple nor direct, since large changes (up to 200-fold) in SCPR accompany relatively small changes (10-fold) in cellular cAMP levels.

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confidence: 99%

Cyclic AMP levels during induction and repression of cellulase biosynthesis in Thermomonospora curvata

Wg¹,

Neubauer²,

Stutzenberger³

1984

J Bacteriol

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“…In fact, a surprising but common observation is that cAMP is present at highest intra-and extracellular levels during periods of rapid growth rather than glucose limited conditions [4,5]. High concentrations 146 of cAMP have also been observed during germination and outgrowth [6]. Thus, in contrast to the dogma elegantly established in Escherichia coli, in Streptomyces it has been claimed that cAMP signals the availability rather than the lack of a readily metabolizable carbon source [4,5,7].…”

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confidence: 99%