1978
DOI: 10.1016/0006-2952(78)90041-2
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Cyclic amp in freshly prepared thymocyte suspensions. evidence for stimulation by endogenous adenosine

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Cited by 30 publications
(13 citation statements)
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“…PhiPrAdo is an adenosine receptor agonist that is not readily transported into cells (13). Consistent with earlier reports of the lymphocyte A2 receptor (7,14), cAMP stimulation by PhiPrAdo was blocked by xanthines (data not shown). Under standard conditions of assay, unstimulated (basal) and PhiPrAdo-stimulated levels of cAMP in normal human lymphocyte preparations (n = 31) were 8.97 ± 0.88 (SEM) and 14.85 ± 1.37 pmol per 106 cells, respectively.…”
Section: Resultssupporting
confidence: 91%
See 1 more Smart Citation
“…PhiPrAdo is an adenosine receptor agonist that is not readily transported into cells (13). Consistent with earlier reports of the lymphocyte A2 receptor (7,14), cAMP stimulation by PhiPrAdo was blocked by xanthines (data not shown). Under standard conditions of assay, unstimulated (basal) and PhiPrAdo-stimulated levels of cAMP in normal human lymphocyte preparations (n = 31) were 8.97 ± 0.88 (SEM) and 14.85 ± 1.37 pmol per 106 cells, respectively.…”
Section: Resultssupporting
confidence: 91%
“…This adaptive response appears to compensate for the acute activating effect of ethanol and may represent a form of cellular tolerance. Adenosine also interacts with receptors on human lymphocytes to stimulate the accumulation of cAMP (7,14,21), raising the possibility that this system might be affected by chronic ethanol abuse in humans. Indeed, lymphocytes from chronic alcoholics had about a 75% reduction in basal and adenosine receptor-stimulated levels of cAMP when compared to lymphocytes from normal individuals or patients with nonalcoholic liver disease.…”
Section: Resultsmentioning
confidence: 99%
“…Lymphocytes release adenosine (22,23), and we find that endogenously released adenosine appears to regulate the response of the adenosine receptor system in cultured lymphocytes (Fig. 1).…”
Section: Discussionmentioning
confidence: 71%
“…Experiments with lymphocytes from alcoholic subjects were conducted simultaneously with lymphocytes from nonalcoholic subjects (laboratory staff and members of a local Mormon church group). Both populations were the same age; alcoholic subjects ranging from 31 to 59 years of age and nonalcoholic subjects from 23 (22,23), extracellular accumulation of adenosine could desensitize and/or down-regulate the adenosine receptor during culture (24,25). This might account for the reduction in adenosine receptor-dependent cAMP levels we observed in cultured lymphocytes relative to freshly isolated lymphocytes.…”
Section: Methodsmentioning
confidence: 99%
“…After 10 min, the supernatant medium was removed, 100 p\ 0.4M per chloric acid added to the cells, and cyclic AM P determined [11].…”
Section: Preparation O F Different Tissue Extractsmentioning
confidence: 99%