Cyclic AMP metabolism in the cell cycle of tetrahymena pyriformis

Dickinson, J. Richard; Graves, M.G.; Swoboda, B.E.P.

doi:10.1016/0014-5793(76)80468-1

Cited by 44 publications

(16 citation statements)

References 18 publications

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“…From this and our previous report [1 ] it appears that the peaks in cyclic AMP and cyclic GMP occur at very nearly the same point in the cell cycle, although the pattern of modulation of the two nucleotides is different. Regulation of important cell cycle events could be accomplished by the independent activation of cyclic GMP-dependent and cyclic AMP-dependent protein kinases.…”

Section: Resultssupporting

confidence: 76%

“…In a recent report on the cyclic AMP metabolism in the cell cycle of Tetrahymena pyriformis we found a large peak in intracellular cyclic AMP coincident with cell division [1 ]. Both cyclic AMP and cyclic GMP have been implicated as important controlling elements in the eukaryotic cell cycle [2].…”

Section: Introductionmentioning

confidence: 67%

“…Tetrahymena pyriformis strain W The growth, method of selection of dividing cells for experimentation and cell counting techniques have all been described previously [1].…”

Section: Selection Era Syncrhonous Population Ofmentioning

confidence: 99%

“…The extraction procedure was essentially the same as that described previously for cyclic AMP [1 ]. Cyclic GMP was separated from cyclic AMP and other nucleotides by chromatography on columns of neutral alumina using 10 mM Tris-HC1, pH 7.4, as eluting buffer.…”

Section: Extraction and Assay Ol Cyclic Gmpmentioning

confidence: 99%

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Cyclic GMP and cyclic GMP phosphodiesterase in the cell cycle of Tetrahymena pyriformis

1976

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Section: Resultssupporting

confidence: 76%

Section: Introductionmentioning

confidence: 67%

“…Tetrahymena pyriformis strain W The growth, method of selection of dividing cells for experimentation and cell counting techniques have all been described previously [1].…”

Section: Selection Era Syncrhonous Population Ofmentioning

confidence: 99%

Section: Extraction and Assay Ol Cyclic Gmpmentioning

confidence: 99%

See 2 more Smart Citations

Cyclic GMP and cyclic GMP phosphodiesterase in the cell cycle of Tetrahymena pyriformis

1976

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“…The whole process takes about 20 hours at 30 ~ and can be performed with large samples of cells at concentrations of 1-2 x 105 cells.ml -I (see ref. 6 for conditions necessary for pairs to form). Thus conjugation in Tetrahymena offers many possibilities for molecular studies of nuclear development including meiosis and nuclear differentiation.…”

Section: Introductionmentioning

confidence: 99%

Magnetic purification of mating Tetrahymena

Bruns

Møller

Leick

1980

Carlsberg Res. Commun.

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The presence of cyclic AMP, and its effects on oral regeneration and in situ ciliary regeneration in stentor coeruleus

Maloney

Burchill

1977

Journal Cellular Physiology

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We have found cyclic AMP in the large, heterotrichous ciliate Stentor coeruleus in amounts per milligram protein similar to those found in another ciliate, Tetrahymena pyriformis. The possible function of cyclic AMP in Stentor was first examined by determining its effects on oral regeneration, the process by which Stentor can replace a missing oral apparatus in eight to ten hours. Once begun (by brief exposure to a 15% sucrose solution, causing shedding of the oral apparatus) regeneration follows eight specific morphological stages visible with the dissecting microscope. Continuous exposure of regenerating cells to either N6, Z'-O-dibutyryl adenosine cyclic 3':5'-monophosphate (DBC) or theophylline begun a t the onset of oral regeneration (stage 0) caused delays in the completion of regeneration. The delays induced by DBC occurred in the early stages prior to stage 5. Regenerating cells exposed to DBC or theophylline at various stages of development were delayed, even at stages 5 and 6. Both DBC and theophylline reversibly bleached the cortical pigment of the cells. Guanosine 3':5'-cyclic monophosphate (cyclic GMP), AMP, GMP, and sodium butyrate neither delayed oral regeneration nor bleached the cortical pigment. Excess extracellular calcium ions alone had no effect on oral regeneration, but 10 mM calcium and DBC caused more delay than DBC alone. Thus, the delay of oral regeneration in Stentor caused by cyclic AMP may involve calcium ions.To determine if cyclic AMP can retard in situ ciliary regeneration by Stentor, as it does in Tetrahymena, a new technique, more accurate than past methods, was developed to monitor ciliary regrowth. Using this procedure we found that both DBC and theophylline significantly delayed the in situ ciliary regeneration by Stentor.

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Cyclic AMP metabolism in the cell cycle of tetrahymena pyriformis

Cited by 44 publications

References 18 publications

Cyclic GMP and cyclic GMP phosphodiesterase in the cell cycle of Tetrahymena pyriformis

Cyclic GMP and cyclic GMP phosphodiesterase in the cell cycle of Tetrahymena pyriformis

Magnetic purification of mating Tetrahymena

The presence of cyclic AMP, and its effects on oral regeneration and in situ ciliary regeneration in stentor coeruleus

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