Cyclic electron flow (CEF) and ascorbate pathway activity provide constitutive photoprotection for the photopsychrophile, Chlamydomonas sp. UWO 241 (renamed Chlamydomonas priscuii)

Stahl-Rommel, Sarah; Kalra, Isha; D’Silva, Susanna; Hahn, Mark M.; Popson, Devon; Cvetkovska, Marina; Morgan‐Kiss, Rachael M.

doi:10.1007/s11120-021-00877-5

Cited by 23 publications

(20 citation statements)

References 98 publications

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“…Constitutive high activity of APX and GR, as well as large Asc pool were observed in Antarctic alga Chlamydomonas sp. UWO 241 (Stahl-Rommel et al 2021 ).…”

Section: Cellular Antioxidantsmentioning

confidence: 99%

Heavy metal–induced stress in eukaryotic algae—mechanisms of heavy metal toxicity and tolerance with particular emphasis on oxidative stress in exposed cells and the role of antioxidant response

Nowicka

2022

Environ Sci Pollut Res

137

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Heavy metals is a collective term describing metals and metalloids with a density higher than 5 g/cm3. Some of them are essential micronutrients; others do not play a positive role in living organisms. Increased anthropogenic emissions of heavy metal ions pose a serious threat to water and land ecosystems. The mechanism of heavy metal toxicity predominantly depends on (1) their high affinity to thiol groups, (2) spatial similarity to biochemical functional groups, (3) competition with essential metal cations, (4) and induction of oxidative stress. The antioxidant response is therefore crucial for providing tolerance to heavy metal-induced stress. This review aims to summarize the knowledge of heavy metal toxicity, oxidative stress and antioxidant response in eukaryotic algae. Types of ROS, their formation sites in photosynthetic cells, and the damage they cause to the cellular components are described at the beginning. Furthermore, heavy metals are characterized in more detail, including their chemical properties, roles they play in living cells, sources of contamination, biochemical mechanisms of toxicity, and stress symptoms. The following subchapters contain the description of low-molecular-weight antioxidants and ROS-detoxifying enzymes, their properties, cellular localization, and the occurrence in algae belonging to different clades, as well as the summary of the results of the experiments concerning antioxidant response in heavy metal-treated eukaryotic algae. Other mechanisms providing tolerance to metal ions are briefly outlined at the end.

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“…Constitutive high activity of APX and GR, as well as large Asc pool were observed in Antarctic alga Chlamydomonas sp. UWO 241 (Stahl-Rommel et al 2021 ).…”

Section: Cellular Antioxidantsmentioning

confidence: 99%

Heavy metal–induced stress in eukaryotic algae—mechanisms of heavy metal toxicity and tolerance with particular emphasis on oxidative stress in exposed cells and the role of antioxidant response

Nowicka

2022

Environ Sci Pollut Res

137

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“…( 6 ) revealed that hundreds of HSDs, involved in diverse cellular processes, are maintained in the psychrophilic Antarctic green alga Chlamydomonas sp. UWO241, which was recently renamed Chlamydomonas priscuii ( 7 ). It is believed that these HSDs are aiding its survival via gene dosage ( 8 ).…”

Section: Introductionmentioning

confidence: 99%

HSDatabase—a database of highly similar duplicate genes from plants, animals, and algae

Zhang

Smith

2022

Database

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Gene duplication is an important evolutionary mechanism capable of providing new genetic material, which in some instances can help organisms adapt to various environmental conditions. Recent studies, for example, have indicated that highly similar duplicate genes (HSDs) are aiding adaptation to extreme conditions via gene dosage. However, for most eukaryotic genomes HSDs remain uncharacterized, partly because they can be hard to identify and categorize efficiently and effectively. Here, we collected and curated HSDs in nuclear genomes from various model animals, land plants and algae and indexed them in an online, open-access sequence repository called HSDatabase. Currently, this database contains 117 864 curated HSDs from 40 distinct genomes; it includes statistics on the total number of HSDs per genome as well as individual HSD copy numbers/lengths and provides sequence alignments of the duplicate gene copies. HSDatabase also allows users to download sequences of gene copies, access genome browsers, and link out to other databases, such as Pfam and Kyoto Encyclopedia of Genes and Genomes. What is more, a built-in Basic Local Alignment Search Tool option is available to conveniently explore potential homologous sequences of interest within and across species. HSDatabase has a user-friendly interface and provides easy access to the source data. It can be used on its own for comparative analyses of gene duplicates or in conjunction with HSDFinder, a newly developed bioinformatics tool for identifying, annotating, categorizing and visualizing HSDs. Database URL: http://hsdfinder.com/database/

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“…Non-model organisms that are adapted to very long-term stress lasting 100s-1000s of years represent under-exploited reservoirs of novel adaptive mechanisms (Dolhi et al, 2013). One such organism is the Antarctic psychrophile Chlamydomonas priscuii UWO241 (UWO241 henceforth), which was isolated from deep photic zone of the ice-covered lake Bonney in the McMurdo Dry Valleys (Morgan et al, 1998; Stahl-Rommel et al, 2021). Extensive studies on this organism have demonstrated that it is a psychrophilic, halotolerant green alga with a reorganized photosynthetic apparatus that includes PSII with a large light harvesting antenna, matched by PSI complexes with diminished antenna size (Dolhi et al, 2013; Morgan-Kiss et al, 2002b; Morgan et al, 1998; Szyszka et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

High salinity activates CEF and attenuates state transitions in both psychrophilic and mesophilic Chlamydomonas species

Kalra

Wang

Zhang

et al. 2022

Preprint

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In the last decade, studies have revealed the importance of PSI-driven cyclic electron flow (CEF) in stress acclimation in model organisms like C. reinhardtii; however, these studies focused on transient, short-term stress. In addition, PSI-supercomplexes are associated with CEF during state transition response to short-term stress. On the other hand, the role of CEF during long-term stress acclimation is still largely unknown. In this study, we elucidate the involvement of CEF in acclimation response to long-term high salinity in three different Chlamydomonas species displaying varying salinity tolerance. We compared CEF rates, capacity for state transitions, and formation of supercomplexes after salinity acclimation in the model mesophile C. reinhardtii and two psychrophilic green algae C. priscuii (UWO241) and C. sp. ICE-MDV. CEF was activated under high salt in all three species, with the psychrophilic Chlamydomonas spp. exhibiting the highest CEF rates. High salt acclimation was also correlated with reduced state transition capacity and a PSI-supercomplex was associated with high CEF. We propose that under long-term stress, CEF is constitutively activated through assembly of a stable PSI-supercomplex. The proteomic composition of the long-term PSI-supercomplex is distinct from the supercomplex formed during state transitions, and its presence attenuates the state transition response.

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Cyclic electron flow (CEF) and ascorbate pathway activity provide constitutive photoprotection for the photopsychrophile, Chlamydomonas sp. UWO 241 (renamed Chlamydomonas priscuii)

Cited by 23 publications

References 98 publications

Heavy metal–induced stress in eukaryotic algae—mechanisms of heavy metal toxicity and tolerance with particular emphasis on oxidative stress in exposed cells and the role of antioxidant response

Heavy metal–induced stress in eukaryotic algae—mechanisms of heavy metal toxicity and tolerance with particular emphasis on oxidative stress in exposed cells and the role of antioxidant response

HSDatabase—a database of highly similar duplicate genes from plants, animals, and algae

High salinity activates CEF and attenuates state transitions in both psychrophilic and mesophilic Chlamydomonas species

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