Cyclic hardening in bundled actin networks

Schmoller, Kurt M.; Fernández, Pablo; Arevalo, Richard; Blair, David F.; Bausch, Andreas R.

doi:10.1038/ncomms1134

Cited by 98 publications

(117 citation statements)

References 20 publications

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“…The interfibrillar contact rupture and reconnection was proposed as a possible mechanism for the increase in ultimate shear stress following cyclic shear loading observed in the actin network with cross-linking proteins [56]. Even though this study did not include a cross-linking agent in the medium during cyclic loading, it is unclear whether the strength or quantity of interfibrillar connections in the DDCS was altered by cyclic loading.…”

Section: Discussionmentioning

confidence: 90%

Collagen network strengthening following cyclic tensile loading

et al. 2016

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The bulk mechanical properties of tissues are highly tuned to the physiological loads they experience and reflect the hierarchical structure and mechanical properties of their constituent parts. A thorough understanding of the processes involved in tissue adaptation is required to develop multi-scale computational models of tissue remodelling. While extracellular matrix (ECM) remodelling is partly due to the changing cellular metabolic activity, there may also be mechanically directed changes in ECM nano/microscale organization which lead to mechanical tuning. The thermal and enzymatic stability of collagen, which is the principal load-bearing biopolymer in vertebrates, have been shown to be enhanced by force suggesting that collagen has an active role in ECM mechanical properties. Here, we ask how changes in the mechanical properties of a collagen-based material are reflected by alterations in the micro/nanoscale collagen network following cyclic loading. Surprisingly, we observed significantly higher tensile stiffness and ultimate tensile strength, roughly analogous to the effect of work hardening, in the absence of network realignment and alterations to the fibril area fraction. The data suggest that mechanical loading induces stabilizing changes internal to the fibrils themselves or in the fibril–fibril interactions. If such a cell-independent strengthening effect is operational in vivo , then it would be an important consideration in any multiscale computational approach to ECM growth and remodelling.

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Section: Discussionmentioning

confidence: 90%

Collagen network strengthening following cyclic tensile loading

et al. 2016

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“…The first group of designs uses counter-rotating surfaces to drive torsional flows with circular streamlines. 25,30,32,[35][36][37][38] Often, one of these surfaces is fixed in the laboratory frame. Independently rotating both surfaces, while more difficult, allows the zero-velocity plane to be moved away from the sample boundaries.…”

Section: Introductionmentioning

confidence: 99%

“…An alternate approach for torsional flows requires mounting a commercial rheometer on a confocal microscope. 25,[36][37][38] To achieve a uniform shear rate, commercial rheometers use a cone and plate geometry in which the sample thickness increases linearly with distance from the rotation axis. Measurements of confined suspensions, however, require a parallel plate geometry in which the sample thickness is uniform but the shear rate is not.…”

Section: Introductionmentioning

confidence: 99%

A multi-axis confocal rheoscope for studying shear flow of structured fluids

Lin

McCoy

Cheng

et al. 2014

Review of Scientific Instruments

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We present a new design for a confocal rheoscope that enables uniform uniaxial or biaxial shear. The design consists of two precisely positioned parallel plates with a gap that can be adjusted down to 2 ±0.1 μm, allowing for the exploration of confinement effects. By using our shear cell in conjunction with a biaxial force measurement device and a high-speed confocal microscope, we are able to measure the real-time biaxial stress while simultaneously imaging the material three-dimensional structure. We illustrate the importance of the instrument capabilities by discussing the applications of this instrument in current and future research topics in colloidal suspensions. © 2014 AIP Publishing LLC.[http://dx

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“…We also quantify a few unavoidable limitations of these systems and confirm some of the capabilities through recent publications. [18][19][20] …”

Section: Introductionmentioning

confidence: 99%

Development of a confocal rheometer for soft and biological materials

Dutta

Mbi

Arevalo

et al. 2013

Review of Scientific Instruments

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We discuss the design and operation of a confocal rheometer, formed by integrating an Anton Paar MCR301 stress-controlled rheometer with a Leica SP5 laser scanning confocal microscope. Combining two commercial instruments results in a system which is straightforward to assemble that preserves the performance of each component with virtually no impact on the precision of either device. The instruments are configured so that the microscope can acquire time-resolved, three-dimensional volumes of a sample whose bulk viscoelastic properties are being measured simultaneously. We describe several aspects of the design and, to demonstrate the system's capabilities, present the results of a few common measurements in the study of soft materials.

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Cyclic hardening in bundled actin networks

Cited by 98 publications

References 20 publications

Collagen network strengthening following cyclic tensile loading

Collagen network strengthening following cyclic tensile loading

A multi-axis confocal rheoscope for studying shear flow of structured fluids

Development of a confocal rheometer for soft and biological materials

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