Cyclin B1 expression in meiotically competent and incompetent goat oocytes

Hue, Isabelle; Dedieu, Thierry; Huneau, D.; Ruffini, Sylvie; Gall, Laurence; Crozet, Nicole

doi:10.1002/(sici)1098-2795(199706)47:2<222::aid-mrd14>3.3.co;2-8

Cited by 7 publications

(9 citation statements)

References 43 publications

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“…Cyclin B is newly synthesized in the presence of actinomycin D 30 min PI, but not in the presence of cycloheximide. Thus it is likely that cyclin B is also newly produced in medaka eggs from cyclin B mRNA that has already been synthesized and accumulated at the end of the growth phase of the oocyte, as reported for the mouse (Hue et al 1997).…”

Section: Discussionmentioning

confidence: 74%

Studies on fertilization of the teleost. II. Nuclear behavior and changes in histone H1 kinase

1999

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In order to understand the dynamic responses of gamete nuclei upon fertilization in the fish, Oryzias latipes, the relationship between changes in the activity of histone H1 kinase and nuclear behavior was examined during fertilization. Kinase activity rapidly decreased concomitant with the initiation of the propagative exocytosis of cortical alveoli following sperm attachment to the egg plasma membrane post-insemination (PI). Activity again increased 30 min PI. Similar changes in kinase activity, migration and syngamy of pronuclei, and subsequent cleavage were observed with aphidicolin or actinomycin D treatment, except that formation of abnormal metaphase chromosomes was retarded in aphidicolin-treated zygotes. Pretreatment of unfertilized eggs with cycloheximide or 6-dimethylaminopurine (6-DMAP) caused no nuclear changes. The activity of histone H1 kinase in these eggs rapidly declined following sperm penetration and exocytosis, but did not undergo subsequent increase in the presence of these inhibitors. In these eggs with low histone H1 kinase activity, the fertilization process from sperm penetration to syngamy occurred normally, but the pronuclear membrane did not break down and the chromosomes did not condense. The present data suggest that in fish eggs, DNA replication as well as the synthesis and phosphorylation of proteins, especially cyclin B, are required for normal formation of metaphase chromosomes at the first cleavage, but not for fertilization events from sperm penetration through to nuclear migration resulting in syngamy.

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Section: Discussionmentioning

confidence: 74%

Studies on fertilization of the teleost. II. Nuclear behavior and changes in histone H1 kinase

1999

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“…In ruminants, cyclin B1 is expressed in granulosa cells and in the oocytes in goat ovary (Hue et al. ), and the involvement of cyclin B1 in oocyte maturation has been reported (Robert et al. ).…”

Section: Introductionmentioning

confidence: 99%

“…In ruminants, cyclin B1 is expressed in granulosa cells and in the oocytes in goat ovary (Hue et al 1997), and the involvement of cyclin B1 in oocyte maturation has been reported (Robert et al 2002). In bovine ovary, the granulosa layer of healthy follicles had a significantly higher frequency of proliferating cell nuclear antigen (PCNA)-positive cells than the early and advanced atretic follicles (Feranil et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Expression of Cyclins and Cyclin‐Dependent Kinase Inhibitors in Granulosa Cells from Bovine Ovary

Shimizu

Hirai

Miyamoto

2013

Reprod Domestic Animals

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The regulation of granulosa cell proliferation is complex, and it is essential for normal follicular development in mammals. The aim of this study was to examine the expression of cyclins and their inhibitors in the granulosa cells of follicles at different developmental stages. Follicles were classified into three groups: oestrogen-inactive dominant follicles (EIDs), oestrogen-active dominant follicles (EADs) and pre-ovulatory follicles (POs). The expression of CCND2 (cyclin D2) mRNA was significantly higher in granulosa cells from EADs and POs than in those from EIDs. The expression of CCND3 (cyclin D3) mRNA was significantly higher in granulosa cells from EADs than in those from other follicles. CCND1 (cyclin D1), CCNE1 (cyclin E1) and CCNE2 (cyclin E2) mRNA expression did not differ among the different follicular stages. The expression of CDKN1A (p21(cip1) ) and CDKN1B (p27(kip1) ) mRNA was significantly higher in granulosa cells from EIDs and POs, respectively, than in those from other follicles. Expression of CDKN2D (p19(INK4d) ) mRNA did not differ among the different follicular stages. Taken together, our study suggested that cyclins and their inhibitors are associated with granulosa cell proliferation at specific follicular developmental stages.

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“…Secondly, the regulation of bovine MPF activity is different from other species and provides a useful experimental system to study cyclin B1 function. In both goat and pig GV stage oocytes, both cyclin B1 and p34 cdc2 protein are detected (Christmann et al, 1994;Hue et al, 1997;Dedieu et al, 1998). In mouse GV stage oocytes cyclin B1 protein can be detected, but it is the translation of p34 cdc2 that is partly associated with meiotic competence (Chesnel and Eppig, 1995;de Vant'ery et al, 1996;de Vantery et al, 1996;Kanatsu-Shinohara et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

RNA interference as a tool to study gene function in bovine oocytes

Paradis¹,

Vigneault²,

Robert³

et al. 2004

Molecular Reproduction Devel

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RNA interference (RNAi) has become a well-established technique to study gene function in several species. Our objective was to develop a RNAi approach to study gene function in bovine oocytes. In the first experiment, three different treatments including a 20 min exposure to cytochalasin B, a 6 hr maturation in cycloheximide, and a combination of these two treatments were tested to improve oocyte survival following microinjection. The cycloheximide/cytochalasin B treatment greatly increased (P<0.02) the survival rate of the microinjected oocytes. In the second experiment, we assessed the effect of both cyclin B1 and GFP dsRNA on cyclin B1 mRNA and protein expression. The injection of cyclin B1 dsRNA resulted in a decrease in cyclin B1 mRNA and protein, while the cyclin B2 mRNA remained unaffected. Furthermore, the injection of GFP dsRNA did not interfere with cyclin B1 mRNA or protein nor with the ability of the oocyte to mature properly. In addition, the lack of cyclin B1 in the oocyte led to activation in 10% of the oocytes as evidenced by the presence of a pronucleus. However, the use of an additional 10 hr of maturation in the presence of 6-dimethylaminopurine (6-DMAP) prevented germinal vesicle breakdown and allowed a longer exposure to dsRNA. This procedure increased the percentage of activated oocytes to 33% and is likely to result from an increased length of time for dsRNA processing and for degradation of the cyclin B1 mRNA to occur. In conclusion, RNAi represents a useful technique to study gene function in the bovine oocyte.

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Cyclin B1 expression in meiotically competent and incompetent goat oocytes

Cited by 7 publications

References 43 publications

Studies on fertilization of the teleost. II. Nuclear behavior and changes in histone H1 kinase

Studies on fertilization of the teleost. II. Nuclear behavior and changes in histone H1 kinase

Expression of Cyclins and Cyclin‐Dependent Kinase Inhibitors in Granulosa Cells from Bovine Ovary

RNA interference as a tool to study gene function in bovine oocytes

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