Cyclodextrin-Modified Capillary Electrophoresis:  Determination of Polycyclic Aromatic Hydrocarbons in Contaminated Soils

Brown, R. Stephen; Luong, John H. T.; Szolar, Oliver H. J.; Halasz, and Annamaria; Hawari, Jalal

doi:10.1021/ac950801y

Cited by 69 publications

(28 citation statements)

References 14 publications

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“…Compared with the sweeping method, the FESI method resulted in 10-fold reduction in detection limits for naphthalene, 20-fold reduction for pyrene, phenanthrene, and fluorine. Also, the detection limit of the present FESI method is almost at the same level or lower than those reported in many papers [14,25].…”

Section: Validationsupporting

confidence: 62%

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Comparison of two capillary electrophoresis online stacking modes by analysis of polycyclic aromatic hydrocarbons in airborne particulates

Song

Peng

Lin

2006

J of Separation Science

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Original PaperComparison of two capillary electrophoresis online stacking modes by analysis of polycyclic aromatic hydrocarbons in airborne particulates Naphthalene, fluorene, pyrene, anthracene, phenanthrene, and chrysene were successfully separated by CD-modified MEKC (CD-MEKC) using 20 mM borate (pH 9.0) containing 90 mM SDS and 75 mM b-CD. Two online stacking methods, i. e., sweeping and field-enhanced sample injection (FESI), were explored to enhance the detection sensitivity. The influences of some crucial parameters in sweeping and FESI procedures were investigated. For FESI method, a plug of water and low-conductivity sample matrix was used to increase the stacking efficiency. Compared with the sweeping method, FESI can increase the sensitivity in the range of 10 -20-fold. The proposed method was used for the analysis of polycyclic aromatic hydrocarbons in airborne particulates.

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Section: Validationsupporting

confidence: 62%

“…Moreover, several other additives were reported, and they included a bile salt surfactant [11], a high-concentration ionic surfactant [12], and a neutral and anionic CD mixture [13]. Among these techniques, CD-modified MEKC (CD-MEKC) has been demonstrated to be an efficient separation method for PAHs [14].…”

Section: Introductionmentioning

confidence: 99%

Comparison of two capillary electrophoresis online stacking modes by analysis of polycyclic aromatic hydrocarbons in airborne particulates

Song

Peng

Lin

2006

J of Separation Science

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“…They obtain baseline separation of 12 hydroxyPAHs with a laserinduced fluorescence (LIF) detection. Cyclodextrin-aided capillary electrophoretic separation and LIF detection were also used to determine mixtures of the 16 US EPA priority pollutants PAHs from contaminated soils [181,182] and the results are comparable to those afforded by HPLC. The indirect fluorescence detection of amino-substituted PAHs in CD-modified MEKC was demonstrated.…”

Section: Micelles and Cyclodextrins In Separation Science Using Fluormentioning

confidence: 96%

Luminescence in organized media and supramolecular interactions:physicochemical aspects and applications

Lerner¹,

Martín²

2000

Analusis

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Introduction Molecular luminescence of analytes in organized media or in supramolecular complexes of cyclodextrinsThe photophysical and photochemical behaviour of molecules complexed to cyclodextrins (CD) is generally different from their behaviour in solution. The first observations in the field were qualitative and were related to the increased stabilisation of labile molecules, mostly drugs, against photodegradation when complexed to natural cyclodextrins [1]. Complexation by CD's was then the preferred subject for those involved in supramolecular chemistry and drug vectorisation techniques [2] as well as in separation techniques [3][4][5]. Stabilization of a molecule against photochemical degradation is still very important in the field of pharmaceutical sciences. A recent example is the stabilisation of promazine as an inclusion complex with β-cyclodextrin [6]. Other photochemists were attracted quite early to this field of research and initiated studies, which were mostly photophysical in nature [7,8]. The key observation was that the formation of supramolecular complexes of analytes with cyclodextrins (CDs) resulted in an increase of their fluorescence quantum yield or even in the appearance of room temperature phosphorescence (RTP) [9][10][11][12]. This enhancement has been used to increase the sensitivity and the selectivity in both luminescence and chromatographic techniques [13-15].We will not say more about RTP with CDs as the subject will be developed in another contribution in the same issue of this Journal. However RTP is also observed in the solubilization of analytes in micellar colloïdal solutions and this field will be reviewed hereunder.These properties have been used to develop analytical techniques, which combine the selectivity of complexation with an enhanced emission [16][17][18][19]. In this way an improvement in the detection limits is obtained in many separation techniques such as HPLC, capillary electrophoresis (CE) or planar chromatography. Furthermore these molecular interactions allow to reveal or to stabilize new molecular forms or to turn species which are normally non-emitting into luminescent ones. All these aspects are reviewed hereunder after a short summary on the nature of the intermolecular interactions, which lie at the heart of these effects. Nature of the interactions between an analyte and a CD or a micelleWithout going into a thorough analysis of the origin of the interactions which exist between molecules in dense media which concern us here, it seems useful to recall the most important factors which should be taken into account when any type of luminescence, fluorescence or phosphorescence is involved.

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“…The separation of the 16 EPA priority PAHs using SB-B-CD and methyl-6-CD in EKC was attempted and reported recently [16]. In this dual cyclodextrin system, most of the 16 PAHs were well separated; however, some PAHs were only partially resolved.…”

Section: Separation Of the Pahs Using Sb-0-cd As The Pseudo-stationarmentioning

confidence: 99%

Mixed‐mode separation of polycyclic aromatic hydrocarbons (PAHs) in electrokinetic chromatography

Luong

Guo

1998

Electrophoresis

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A mixed-mode separation technique has been developed and optimized for the separation of the 16 Environmental Protection Agency (EPA) priority polycyclic aromatic hydrocarbons (PAHs). The procedure utilized two different buffer additives as pseudo-stationary phases with different selectivities towards the analytes. Sodium dioctyl sulfosuccinate (DOSS) displayed selectivities for PAHs which were somewhat similar to the C18 phase in reversed-phase high performance liquid chromatography (HPLC). High acetonitrile content required for an effective separation prevented the formation of micelles as confirmed by fluorescence spectroscopy. Consequently, the separation could be attributed to the solvophobic association of the PAH molecules with hydrophobic chains of the DOSS surfactant. In another mode of separation, sulfobutylether-beta-cyclodextrin (SB-beta-CD) separated the 16 PAHs on the formation of inclusion complexes with the PAHs, and exhibited different selectivities for the PAHs compared to DOSS. SB-beta-CD and DOSS were then combined in the running buffer to form a mixed pseudo-stationary phase for the separation of the 16 PAHs. Due to the different selectivities of SB-beta-CD and DOSS for the PAHs, the separation of the 16 PAHs was appreciably improved compared to that using DOSS or SB-beta-CD alone. All the 16 PAHs were baseline-resolved using an optimized running buffer containing 22.5 mM DOSS, 15 mM SB-beta-CD, 15% acetonitrile and 5 mM hydroxypropyl-beta-cyclodextrin in 6 mM borate at pH 9.

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Cyclodextrin-Modified Capillary Electrophoresis: Determination of Polycyclic Aromatic Hydrocarbons in Contaminated Soils

Cited by 69 publications

References 14 publications

Comparison of two capillary electrophoresis online stacking modes by analysis of polycyclic aromatic hydrocarbons in airborne particulates

Comparison of two capillary electrophoresis online stacking modes by analysis of polycyclic aromatic hydrocarbons in airborne particulates

Luminescence in organized media and supramolecular interactions:physicochemical aspects and applications

Mixed‐mode separation of polycyclic aromatic hydrocarbons (PAHs) in electrokinetic chromatography

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