Cyclooxygenase-2 and angiogenesis in prostate cancer

Tkacz, Victoria L.; Tohnya, Tanyifor M.; Figg, William D.

doi:10.4161/cbt.4.8.2089

Cited by 10 publications

(5 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Curcumin also downregulates NF-B induced COX-2 (Tunstall et al, 2006) and MMP-2 expression and inhibits cell migration (Su et al, 2006). COX-2 has been found to be overexpressed in several human cancers (Chan et al, 1999;Chun et al, 2003;Csiki et al, 2005;Heeren et al, 2005;Mascaux et al, 2005;Peng et al, 2005;Samoha and Arber, 2005;Tkacz et al, 2005;Wadhwa et al, 2005;Yasuda et al, 2005).…”

Section: Introductionmentioning

confidence: 97%

Curcumin down regulates smokeless tobacco-induced NF-κB activation and COX-2 expression in human oral premalignant and cancer cells

et al. 2006

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 97%

Curcumin down regulates smokeless tobacco-induced NF-κB activation and COX-2 expression in human oral premalignant and cancer cells

et al. 2006

View full text Add to dashboard Cite

“…Inflamed neoplastic cells secrete excess IL-8, which enhances the growth and tumorigenicity in human prostate cancer mediated by the induction of MMP-9 expression 27, 28, 29, 30, 31, 32, 33, 34. When compared with normal tissues, malignant tissues were found to induce COX-2 expression constitutively; this remains throughout the process from dysplasia to metastasis.…”

Section: Discussionmentioning

confidence: 99%

Withania somnifera targets interleukin-8 and cyclooxygenase-2 in human prostate cancer progression

Balakrishnan

Nathan

Kumar

et al. 2017

Prostate International

View full text Add to dashboard Cite

BackgroundProstate cancer (PC) is a common noncutaneous malignancy in men. The incidence of PC is increasing at an alarming rate across the globe. Progression of PC is associated with elevated levels of interleukin-8 (IL-8) and cyclooxygenase-2 (COX-2) in malignant cells. Overexpression of these players is accompanied by chronic inflammation, increased angiogenesis, proliferation, migration, and inhibition of apoptosis. Moreover, their elevated circulating levels promote the disease progression from androgen-dependent to androgen-independent state. Thus, inhibiting the expression of IL-8 and COX-2 would be a promising target in the development of PC therapeutics. In this study, we investigated the inhibitory effects of Withania somnifera extract on highly metastatic, androgen-independent prostate cancer cell line (PC3). Additionally, we compared the real-time expression of IL-8 and COX-2 in prostate tissue samples.Materials and methodsThe cell viability and cytotoxicity of W. somnifera extract in PC3 cells was quantified colorimetrically by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide and lactate dehydrogenase leakage assay, respectively. Hematoxylin and eosin staining for histological examination, trypan blue, and acridine orange dyes to enumerate apoptotic and live cells, quantitative real-time polymerase chain reaction to determine the expression and flow cytometry to study the cell cycle analysis were used.ResultsWe observed a significant decrease in the cell viability with a half-maximal inhibitory concentration (IC50) of 10 μg/mL. The expression levels of IL-8 and COX-2 in prostate tissue samples and in PC3 cells were predominantly high; however, the lowest dose of W. somnifera significantly inhibited the enhanced expression of IL-8 and COX-2 in PC3 cells in 24 hours. Furthermore, W. somnifera extract (10 μg/mL) irreversibly arrested the cell cycle in G2/M phase, which was evident from the rapid accumulation of PC3 cells significantly.ConclusionOur results indicate that inherent metastatic and selective inhibitory potential of W. somnifera against PC. W. somnifera may be a good therapeutic agent in addition to the existing drugs for PC. Further studies with more prostate tissue samples are warranted.

show abstract

“…There is also evidence from population based, case control and clinical trials that regular use of NSAIDs may reduce the relative risk to develop colorectal adenomas (Baron et al 2003; Sandler et al 2003) and colorectal cancer (Smalley and DuBois 1997; Thun et al 1991; Williams et al 1999; Giovannucci et al 1994; Giovannucci et al 1995; Smalley et al 1999). Altered expression of COX-2 and overproduction of prostaglandins are common in colorectal cancers (Eberhart et al 1994; Marnett and DuBois 2002; Williams et al 1999; Joo et al 2002), breast (Rolland et al 1980), gastric (Uefuji et al 2001), esophagus (Shamma et al 2000), pancreatic (Juuti et al 2006), bile duct (Hayashi et al 2001), papillary thyroid (Siironen et al 2006), malignant pheochromocytomas (Salmenkivi et al 2001), urinary tract (Tuna et al 2004), prostate (Tkacz et al 2005), cervical (Kulkarni et al 2001), retinoblastoma (Karim et al 2000) and lung cancer (Wolff et al 1998). Elevated levels of COX-2 and PGE 2 are also seen in premalignant conditions such as Barrett′s esophagus (Kandil et al 2001) and colorectal adenomas (Khan et al 2001).…”

Section: Discussionmentioning

confidence: 99%

Global Tumor RNA Expression in Early Establishment of Experimental Tumor Growth and Related Angiogenesis following Cox-Inhibition Evaluated by Microarray Analysis

et al. 2007

View full text Add to dashboard Cite

Altered expression of COX-2 and overproduction of prostaglandins, particularly prostaglandin E 2 , are common in malignant tumors. Consequently, non-steroidal anti-infl ammatory drugs (NSAIDs) attenuate tumor net growth, tumor related cachexia, improve appetite and prolong survival. We have also reported that COX-inhibition (indomethacin) interfered with early onset of tumor endothelial cell growth, tumor cell proliferation and apoptosis. It is however still unclear whether such effects are restricted to metabolic alterations closely related to eicosanoid pathways and corresponding regulators, or whether a whole variety of gene products are involved both up-and downstream effects of eicosanoids. Therefore, present experiments were performed by the use of an in vivo, intravital chamber technique, where micro-tumor growth and related angiogenesis were analyzed by microarray to evaluate for changes in global RNA expression caused by indomethacin treatment. Indomethacin up-regulated 351 and down-regulated 1852 genes signifi cantly (p < 0.01); 1066 of these genes had unknown biological function. Genes with altered expression occurred on all chromosomes. Our results demonstrate that indomethacin altered expression of a large number of genes distributed among a variety of processes in the carcinogenic progression involving angiogenesis, apoptosis, cell-cycling, cell adhesion, infl ammation as well as fatty acid metabolism and proteolysis. It remains a challenge to distinguish primary key alterations from secondary adaptive changes in transcription of genes altered by cyclooxygenase inhibition.

show abstract

Cyclooxygenase-2 and angiogenesis in prostate cancer

Cited by 10 publications

References 14 publications

Curcumin down regulates smokeless tobacco-induced NF-κB activation and COX-2 expression in human oral premalignant and cancer cells

Curcumin down regulates smokeless tobacco-induced NF-κB activation and COX-2 expression in human oral premalignant and cancer cells

Withania somnifera targets interleukin-8 and cyclooxygenase-2 in human prostate cancer progression

Global Tumor RNA Expression in Early Establishment of Experimental Tumor Growth and Related Angiogenesis following Cox-Inhibition Evaluated by Microarray Analysis

Contact Info

Product

Resources

About