Cyclooxygenase-2 Derived PGE2 and PGI2 Play an Important Role Via EP2 and PPARδ Receptors in Early Steps of Oil Induced Decidualization in Mice

Pakrasi, Pranab L.; Jain, Anil K.

doi:10.1016/j.placenta.2008.03.001

Cited by 42 publications

(34 citation statements)

References 38 publications

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“…In our study, we did find a significant increase for RXRA protein and a trend for increased PPARD protein at 16 and 32 h after the deciduogenic stimulus. Our results are in agreement with Pakrasi & Jain (2008) where in mice PPARD protein expression is significantly higher 24 h after deciduogenic stimulus was given. However, it is important to highlight that by that time, the increase in EVP and the first differentiation of decidual cells would have occurred, suggesting that these PPARD and RXRA receptors, if they have a role in rodents, may only be in the later stages of decidualization.…”

Section: Discussionsupporting

confidence: 92%

“…In rats, the increase in EVP occurs late in the afternoon of day 5, whereas in hamsters and mice, it occurs in the afternoon and the evening of day 4 respectively. Previous studies in mice and hamsters have suggested a role of PTGS2, but not of PTGS1, in implantation (Chakraborty et al 1996, Lim et al 1999, Wang et al 2004, Pakrasi & Jain 2008 and also in decidualization and placentation in mice (Lim et al 1997, Wang et al 2007, Pakrasi & Jain 2008. The presence of Ptgs1 transcripts in the rat endometrium prior to implantation is in agreement with the hamster study ( Wang et al 2004), where it was detected as early as days 1 and 3, before the initiation of implantation occurs.…”

Section: Discussionsupporting

confidence: 83%

“…On the afternoon of day 4 and the morning of day 5, after implantation has been initiated in hamsters, PTGS2 is detected in epithelial and in several layers of stromal cells adjacent to the blastocysts ( Wang et al 2004). The results in rats also differ from those in mice, where PTGS2 was localized to the luminal epithelium, underlying stroma and in glandular epithelium 24 h after a deciduogenic stimulus was given (at the time when decidualization would have started) and then dramatically decrease at 48 and 120 h (Pakrasi & Jain 2008). In our study, as well as in those of Wang et al (2004) and Cong et al (2006), there was a gradual increase in the number of PTGS2 positive stromal cells as shown by immunostaining.…”

Section: Discussionmentioning

confidence: 80%

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Expression patterns and role of prostaglandin-endoperoxide synthases, prostaglandin E synthases, prostacyclin synthase, prostacyclin receptor, peroxisome proliferator-activated receptor delta and retinoid x receptor alpha in rat endometrium during artificially-induced decidualization

Gillio-Meina¹,

Phang²,

Mather³

et al. 2009

Reproduction

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To determine if changes in endometrial expression of the enzymes and receptors involved in prostaglandin (PG) synthesis and action might provide insights into the PGs involved in the initiation of decidualization, ovariectomized steroid-treated rats at the equivalent of day 5 of pseudopregnancy were given a deciduogenic stimulus and killed at various times up to 32 h thereafter. The expression of PG-endoperoxide synthases (PTGS1 and PTGS2), microsomal PGE synthases (PTGES and PTGES2), cytosolic PGE synthase (PTGES3), prostacyclin synthase (PTGIS), prostacyclin receptor, peroxisome proliferator-activated receptor d (PPARD) and retinoid x receptor a (RXRA) in endometrium was assessed by semiquantitative RT-PCR, western blot analyses and immunohistochemistry. In addition, to determine which PG is involved in mediating decidualization, we compared the ability of PGE 2 , stable analogues of PGI 2 , L165041 (an agonist of PPARD), and docasahexanoic acid (an agonist of RXRA) to increase endometrial vascular permeability (EVP, an early event in decidualization), and decidualization when infused into the uterine horns of rats sensitized for the decidual cell reaction (DCR). EVP was assessed by uterine concentrations of Evans blue 10 h after initiation of infusions. DCR was assessed by the uterine mass 5 days after the initiation of the infusions. Because enzymes associated with the synthesis of PGE 2 , including PTGS2, are up-regulated in response to a deciduogenic stimulus and because PGE 2 was more effective than the PGI 2 analogues and PPARD and RXRA agonists in increasing EVP and inducing decidualization, we suggest that PGE 2 is most likely the PG involved in the initiation of decidualization in the rat.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 80%

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Expression patterns and role of prostaglandin-endoperoxide synthases, prostaglandin E synthases, prostacyclin synthase, prostacyclin receptor, peroxisome proliferator-activated receptor delta and retinoid x receptor alpha in rat endometrium during artificially-induced decidualization

Gillio-Meina¹,

Phang²,

Mather³

et al. 2009

Reproduction

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“…It has been reported that PGE 2 promotes decidualization via EP3 and EP4 in rat ESCs (Papay & Kennedy 2000). Furthermore, mouse ESCs differentiated into decidualized cells in response to elevated cAMP levels, which is mediated by PGE 2 via EP2 (Pakrasi & Jain 2008). Thus, activation of cAMP signaling is crucial for decidualization in rodents and humans.…”

Section: Discussionmentioning

confidence: 96%

Possible roles of the cAMP-mediators EPAC and RAP1 in decidualization of rat uterus

Kusama¹,

Yoshie²,

Tamura³

et al. 2014

Reproduction

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The optimal decidualization of endometrial stromal cells (ESCs) following embryo implantation is one of the critical steps to establish pregnancy in rodents and humans. This step is intricately regulated by ovarian hormones. Using in vitro human ESCs model, we previously showed that activation of a cAMP mediator, exchange protein directly activated by cAMP (EPAC), promotes ovarian steroid-or cAMP analog-induced decidualization. However, expressions and functions of EPAC and RAP1 in the uterus during pregnancy have not yet been examined. In this study, we found that the expression of EPAC2 and RAP1 was markedly upregulated in the decidual cells at the implantation sites on days 7 and 9 of pregnancy in rats. Furthermore, both delayed-implantation and artificial decidualization models showed that EPAC2 and RAP1 expression was enhanced in decidual cells. Significant activation of cAMP-responsive element-binding protein (CREB), a central transcriptional factor of cAMP signaling, was observed in decidual cells. These spatiotemporal expressions of protein related EPAC pathway are overlapped by sites with activated cAMP signaling, indicating the association of EPAC signaling with decidualization. Strikingly, further studies in in vitro rat decidualization model showed that the cAMP analog and medroxyprogesterone stimulated the expression of decidual markers, while knockdown of EPAC1/2 and RAP1 attenuated the expressions of these markers. Together, these findings suggest that EPAC and RAP1 are the crucial factors for endometrial decidualization in rat pregnancy.

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“…25 Under artificially induced decidualization, Cox-2 inhibitor nimesulide diminished the production of uterine PGE 2 which was important for decidualization. 26,27 mPGES-1 might catalyze the conversion of PGH 2 to PGE 2 and was also modulated by Hmgn1 during in vitro decidualization. 26 Cumulative evidences have declared that deficiency of Cox-2 or mPGES-1 caused a reduction in uterine Vegf mRNA level.…”

Section: Discussionmentioning

confidence: 99%

Hmgn1 acts downstream of C/EBPβ to regulate the decidualization of uterine stromal cells in mice

Yang

Guo

et al. 2015

Cell Cycle

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Cyclooxygenase-2 Derived PGE2 and PGI2 Play an Important Role Via EP2 and PPARδ Receptors in Early Steps of Oil Induced Decidualization in Mice

Cited by 42 publications

References 38 publications

Expression patterns and role of prostaglandin-endoperoxide synthases, prostaglandin E synthases, prostacyclin synthase, prostacyclin receptor, peroxisome proliferator-activated receptor delta and retinoid x receptor alpha in rat endometrium during artificially-induced decidualization

Expression patterns and role of prostaglandin-endoperoxide synthases, prostaglandin E synthases, prostacyclin synthase, prostacyclin receptor, peroxisome proliferator-activated receptor delta and retinoid x receptor alpha in rat endometrium during artificially-induced decidualization

Possible roles of the cAMP-mediators EPAC and RAP1 in decidualization of rat uterus

Hmgn1 acts downstream of C/EBPβ to regulate the decidualization of uterine stromal cells in mice

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