Cyclooxygenase-2 utilizes Jun N-terminal kinases to induce invasion, but not tamoxifen resistance, in MCF-7 breast cancer cells

González-Villasana, Vianey; Gutierrez-Puente, Yolanda; Tari, Ana M.

doi:10.3892/or.2013.2549

Cited by 11 publications

(7 citation statements)

References 31 publications

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“…Jun is activated through phosphorylation at Ser 63 and Ser 73 by JNK [92,93]. A high level of Jun has been observed in various types of cancer including non-small cell lung cancer, oral squamous cell carcinoma, breast cancer and colorectal cancer [94,95,96,97,98]. Overexpression of Jun has led to aberrant tumor growth and progression and inhibited cell apoptosis [94].…”

Section: Resultsmentioning

confidence: 99%

Determination of Genes Related to Uveitis by Utilization of the Random Walk with Restart Algorithm on a Protein–Protein Interaction Network

Yan

et al. 2017

IJMS

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Uveitis, defined as inflammation of the uveal tract, may cause blindness in both young and middle-aged people. Approximately 10–15% of blindness in the West is caused by uveitis. Therefore, a comprehensive investigation to determine the disease pathogenesis is urgent, as it will thus be possible to design effective treatments. Identification of the disease genes that cause uveitis is an important requirement to achieve this goal. To begin to answer this question, in this study, a computational method was proposed to identify novel uveitis-related genes. This method was executed on a large protein–protein interaction network and employed a popular ranking algorithm, the Random Walk with Restart (RWR) algorithm. To improve the utility of the method, a permutation test and a procedure for selecting core genes were added, which helped to exclude false discoveries and select the most important candidate genes. The five-fold cross-validation was adopted to evaluate the method, yielding the average F1-measure of 0.189. In addition, we compared our method with a classic GBA-based method to further indicate its utility. Based on our method, 56 putative genes were chosen for further assessment. We have determined that several of these genes (e.g., CCL4, Jun, and MMP9) are likely to be important for the pathogenesis of uveitis.

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Section: Resultsmentioning

confidence: 99%

Determination of Genes Related to Uveitis by Utilization of the Random Walk with Restart Algorithm on a Protein–Protein Interaction Network

Yan

et al. 2017

IJMS

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“…However, there also existed an experiment identified that this function did not perform effects. They found that Cox‐2 stimulated the activity of protein kinase C ( PKC ) to enhance the phosphorylation of Jun N‐terminal kinases ( JNKs ), but not that of AKT family members in estrogen receptor ( ER ) positive cells [29]. This difference might due to the different microenvironments between various cells.…”

Section: Discussionmentioning

confidence: 99%

miR‐137 effects on gastric carcinogenesis are mediated by targeting Cox‐2‐activated PI3K/AKT signaling pathway

Cheng

Liu

et al. 2014

FEBS Letters

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Edited by Varda RotterKeywords: Stomach neoplasm miR-137 Cyclooxygenase-2 PI3K/AKT signaling pathway Xenograft tumor a b s t r a c tThe discovery of microRNAs (miRNAs) provided a new avenue for early diagnosis and treatment of GC. MiR-137 has been reported to be under-expressed and involved in various cell processes. However, the role of miR-137 in GC is less known. In this study, we show that miR-137 is under-expressed in GC and functions as a tumor suppressor through targeting Cyclooxygenase-2 (Cox-2), which subsequently suppresses the activation of PI3K/AKT signaling pathway both in vitro and in vivo. Moreover, restored Cox-2 expression partially abolished the tumor suppressive effects of miR-137 in GC cells, suggesting miR-137 may suppress GC carcinogenesis by targeting Cox-2.

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“…4 × 105 cells were pretreated with the JNK inhibitor SP600125 (0, 5 or 10 μM) in RPMI 1640 medium containing 5% FBS for 30 min before being added to the Matrigel-coated Transwell inserts. The cells, migrated for 12 h, were fixed and counted in 10 high-powered (×200) fields under a microscope as mentioned by Gonzalez-Villasana V et al [22]. The experiment was repeated three times.…”

Section: Methodsmentioning

confidence: 99%

Dickkopf-4 is frequently overexpressed in epithelial ovarian carcinoma and promotes tumor invasion

Wang¹,

Wei²,

Zhang³

2017

BMC Cancer

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BackgroundDickkopf-4 (DKK4), a member of DKK family, appears to be a divergent protein. It remained multi-biological functions in carcinogenesis. The effect of DKK4 on the ovarian cancer cells remains unclear. This study detected the clinical significance of DKK4 in epithelial ovarian cancer (EOC) patients and its role in invasion.MethodsQRT-PCR and western blot analysis were used to examine the levels of DKK4 mRNA and protein in 33 EOC tissues and 33 benign ovarian tumors. Immunohistochemical analysis was performed to assess DKK4 expression in 239 EOC samples. siRNA-mediated DKK4 silence was conducted. Transwell assay was used to detect the invasive ability. Phalloidin was used to stain the formations of actin filaments.ResultsThe expressions of DKK4 mRNA and protein were elevated in EOC tissues as compared with those in benign ovarian tumors (p = 0.001 and <0.0001 respectively). Immunohistochemical results showed the strong expression of DKK4 protein was positively associated with late FIGO stage (p = 0.005) and poor disease free survival in univariate and multivariate analysis (p < 0.0001 and p = 0.001, respectively). SiRNA-mediated DKK4 knockdown inhibited cell invasive ability (all p < 0.0001) and the formations of actin filaments. DKK4 could promote the phosphration of c-JUN and JNK (p < 0.0001 and p = 0.001, respectively).ConclusionsOur results indicated that DKK4 might be contributed to predicting EOC progression and prognosis. DKK4 could promote the invasion of EOC through JNK activation.

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Cyclooxygenase-2 utilizes Jun N-terminal kinases to induce invasion, but not tamoxifen resistance, in MCF-7 breast cancer cells

Cited by 11 publications

References 31 publications

Determination of Genes Related to Uveitis by Utilization of the Random Walk with Restart Algorithm on a Protein–Protein Interaction Network

Determination of Genes Related to Uveitis by Utilization of the Random Walk with Restart Algorithm on a Protein–Protein Interaction Network

miR‐137 effects on gastric carcinogenesis are mediated by targeting Cox‐2‐activated PI3K/AKT signaling pathway

Dickkopf-4 is frequently overexpressed in epithelial ovarian carcinoma and promotes tumor invasion

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