Cyclops‐independent floor plate differentiation in zebrafish embryos

Albert, Sabrina; Müller, Ferenc; Fischer, Nadine; Biellmann, Dominique; Neumann, Carl J.; Blader, Patrick; Strähle, Uwe

doi:10.1002/dvdy.10211

Cited by 19 publications

(19 citation statements)

References 62 publications

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“…In Nodal 2/2 mutants, the floor plate is prematurely induced in the anterior epiblast in mouse embryos (Camus et al 2006), whereas inactivation of the gene encoding the nodal antagonist Lefty increases induction of mesendoderm in formerly ectodermal tissue (Meno et al 1999;Perea-Gomez et al 2002). Nodal-deficient mutants show late recovery of midline floor plate cells in the trunk and tail, identifying signaling pathways that ameliorate these effects, specifically Shh and Gli activity (Albert et al 2003;Norton et al 2005). This late recovery, however, is not observed in the brain.…”

Section: Neural Patterningmentioning

confidence: 99%

TGF-β Family Signaling in Neural and Neuronal Differentiation, Development, and Function

Meyers

Kessler

2017

Cold Spring Harb Perspect Biol

137

107

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Section: Neural Patterningmentioning

confidence: 99%

TGF-β Family Signaling in Neural and Neuronal Differentiation, Development, and Function

Meyers

Kessler

2017

Cold Spring Harb Perspect Biol

137

107

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“…2AЉ). Therefore, although Hh signalling is required to maintain medial floor plate (Albert et al, 2003), it is not required for the initial formation of this structure.…”

Section: Medial Floorplate Still Forms In the Absence Of Hh Signallingmentioning

confidence: 99%

Roles of Hedgehog pathway components and retinoic acid signalling in specifying zebrafish ventral spinal cord neurons

et al. 2011

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SUMMARYIn mouse, Hedgehog (Hh) signalling is required for most ventral spinal neurons to form. Here, we analyse the spinal cord phenotype of zebrafish maternal-zygotic smoothened (MZsmo) mutants that completely lack Hh signalling. We find that most V3 domain cells and motoneurons are lost, whereas medial floorplate still develops normally and V2, V1 and V0v cells form in normal numbers. This phenotype resembles that of mice that lack both Hh signalling and Gli repressor activity. Ventral spinal cord progenitor domain transcription factors are not expressed at 24 hpf in zebrafish MZsmo mutants. However, pMN, p2 and p1 domain markers are expressed at early somitogenesis stages in these mutants. This suggests that Gli repressor activity does not extend into zebrafish ventral spinal cord at these stages, even in the absence of Hh signalling. Consistent with this, ectopic expression of Gli3R represses ventral progenitor domain expression at these early stages and knocking down Gli repressor activity rescues later expression. We investigated whether retinoic acid (RA) signalling specifies ventral spinal neurons in the absence of Hh signalling. The results suggest that RA is required for the correct number of many different spinal neurons to form. This is probably mediated, in part, by an effect on cell proliferation. However, V0v, V1 and V2 cells are still present, even in the absence of both Hh and RA signalling. We demonstrate that Gli1 has a Hh-independent role in specifying most of the remaining motoneurons and V3 domain cells in embryos that lack Hh signalling, but removal of Gli1 activity does not affect more dorsal neurons.

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“…1). We used transgenic embryos that express the green fluorescent protein (gfp) gene under control of the regulatory elements of the shh gene (Albert et al 2003). At the 6-10-somite stage, these transgenic embryos express the gfp reporter gene in the notochord, the prechordal plate, and in the ventral midline of the neural plate comprising the prospective floor plate and hypothalamus ( Fig.…”

Section: Preparation Of a Complex Probe From Midline Cellsmentioning

confidence: 99%

“…For each locus, the coding region with the intronic sequences, 10 kb upstream of the ATG and 10 kb downstream from the stop codon, were searched for homologies with the "Compare" and "DotPlot" programs (GCG Wisconsin package). The settings of the search algorithm were kept at the default values, which allowed scoring the regulatory sequences of the zebrafish ngn1 and shh genes (Muller et al 1999(Muller et al , 2000Albert et al 2003). Among 45 pairwise comparisons, 18 homologies were detected in the noncoding regions of 10 genes ( Fig.…”

Section: Comparative Sequence Analysis Of Homologous Loci In the Takimentioning

confidence: 99%

“…Several regulatory regions with activity in the floor plate or notochord were previously identified in the zebrafish shh and netrin1 genes (Muller et al 1999;Rastegar et al 2002;Albert et al 2003). We compared the sequence of the ctgf up1 region with the floor plate/notochord enhancers ar-A, ar-B, and ar-C of the shh, and the FPE of the netrin1 gene (Figs.…”

Section: The Regulatory Region Of the Ctgf Gene Shares Homology With mentioning

confidence: 99%

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Expression Profiling and Comparative Genomics Identify a Conserved Regulatory Region Controlling Midline Expression in the Zebrafish Embryo

Dickmeis¹,

Plessy²,

Rastegar³

et al. 2004

Genome Res.

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Differential gene transcription is a fundamental regulatory mechanism of biological systems during development, body homeostasis, and disease. Comparative genomics is believed to be a rapid means for the identification of regulatory sequences in genomes. We tested this approach to identify regulatory sequences that control expression in the midline of the zebrafish embryo. We first isolated a set of genes that are coexpressed in the midline of the zebrafish embryo during somitogenesis stages by gene array analysis and subsequent rescreens by in situ hybridization. We subjected 45 of these genes to Compare and DotPlot analysis to detect conserved sequences in noncoding regions of orthologous loci in the zebrafish and Takifugu genomes. The regions of homology that were scored in nonconserved regions were inserted into expression vectors and tested for their regulatory activity by transient transgenesis in the zebrafish embryo. We identified one conserved region from the connective tissue growth factor gene (ctgf), which was able to drive expression in the midline of the embryo. This region shares sequence similarity with other floor plate/notochord-specific regulatory regions. Our results demonstrate that an unbiased comparative approach is a relevant method for the identification of tissue-specific cis-regulatory sequences in the zebrafish embryo.[Supplemental material is available online at www.genome.org. The sequence data from this study have been submitted to GenBank under accession nos. AY428026-AY428035, CD777505-CD777543, and CD777544-CD778191.]Differential gene transcription is essential for many developmental processes and physiological responses. Moreover, evolution of the body plan is thought to have been driven to a large extent by changes in gene expression, and differences in gene expression are believed to determine disease risk (Davidson 2001; Ludwig 2002 and references therein). A detailed knowledge of the cisregulatory architecture of genomes is thus a central prerequisite for a comprehensive understanding of many fundamental biological processes and has also medical implications.Despite enormous progress in our understanding of transcriptional regulation in vertebrates (Davidson 2001), we are not able to unequivocally predict the pattern and responsiveness of expression by mere sequence analysis of a gene. This is largely because of the structure and the scattered location of cisregulatory regions in the huge vertebrate genomes (Davidson 2001). In vertebrates, promoter regions are rarely sufficient to drive faithful gene transcription. Frequently, other regulatory regions contribute to the correct spatial and temporal expression of genes. These regions can be scattered over large distances in noncoding sequences, and they can be located upstream, downstream, and in intronic regions (Davidson 2001). Regulatory regions are usually a composite of multiple transcription-factorbinding sites. The small size and degenerate DNA sequence of these binding sites renders a genome-wide computational analys...

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Cyclops‐independent floor plate differentiation in zebrafish embryos

Cited by 19 publications

References 62 publications

TGF-β Family Signaling in Neural and Neuronal Differentiation, Development, and Function

TGF-β Family Signaling in Neural and Neuronal Differentiation, Development, and Function

Roles of Hedgehog pathway components and retinoic acid signalling in specifying zebrafish ventral spinal cord neurons

Expression Profiling and Comparative Genomics Identify a Conserved Regulatory Region Controlling Midline Expression in the Zebrafish Embryo

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