Cyclosorus terminans Extract Ameliorates Insulin Resistance and Non-Alcoholic Fatty Liver Disease (NAFLD) in High-Fat Diet (HFD)-Induced Obese Rats

Songtrai, Sujinda; Pratchayasakul, Wasana; Arunsak, Busarin; Chunchai, Titikorn; Kongkaew, Aphisek; Chattipakorn, Nipon; Chattipakorn, Nipon; Kaewsuwan, Sireewan

doi:10.3390/nu14224895

Cited by 9 publications

(5 citation statements)

References 65 publications

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“…Therefore, it cannot be excluded that GW9662 partially blocks PPARα, contributing to the reversion of the anti-steatotic effect of BCP observed in our experiments. In accordance with recently published results [ 40 ], we found that both PPARα and γ are downregulated during the pathological condition of steatosis. Intriguingly, co-treatment with BCP induced a marked upregulation of PPARα, bringing it back to even higher expression levels than in the untreated control.…”

Section: Discussionsupporting

confidence: 93%

Beta-Caryophyllene Modifies Intracellular Lipid Composition in a Cell Model of Hepatic Steatosis by Acting through CB2 and PPAR Receptors

Scandiffio

Bonzano

Cottone

et al. 2023

IJMS

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Non-alcoholic fatty liver disease (NAFLD) is the most common cause of chronic liver disease; however, no specific pharmacological therapy has yet been approved for this condition. Plant-derived extracts can be an important source for the development of new drugs. The aim of this study was to investigate the effects of (E)-β-caryophyllene (BCP), a phytocannabinoid recently found to be beneficial against metabolic diseases, on HepG2 steatotic hepatocytes. Using a fluorescence-based lipid quantification assay and GC-MS analysis, we show that BCP is able to decrease lipid accumulation in steatotic conditions and to change the typical steatotic lipid profile by primarily reducing saturated fatty acids. By employing specific antagonists, we demonstrate that BCP action is mediated by multiple receptors: CB2 cannabinoid receptor, peroxisome proliferator-activated receptor α (PPARα) and γ (PPARγ). Interestingly, BCP was able to counteract the increase in CB2 and the reduction in PPARα receptor expression observed in steatotic conditions. Moreover, through immunofluorescence and confocal microscopy, we demonstrate that CB2 receptors are mainly intracellularly localized and that BCP is internalized in HepG2 cells with a maximum peak at 2 h, suggesting a direct interaction with intracellular receptors. The results obtained with BCP in normal and steatotic hepatocytes encourage future applications in the treatment of NAFLD.

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Section: Discussionsupporting

confidence: 93%

Beta-Caryophyllene Modifies Intracellular Lipid Composition in a Cell Model of Hepatic Steatosis by Acting through CB2 and PPAR Receptors

Scandiffio

Bonzano

Cottone

et al. 2023

IJMS

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“…Several plant extracts, including those derived from Hibiscus sabdariffa, Panax notoginseng, Antidesma bunius, Curcuma longa Linn., Aralia elata, Cyclosorus terminans, Pluchea indica, and R. officinalis Linn. (Table IV), upregulated the expression leels of SREBP-1c, FAS, ACC, and CPT-1, as well as PPAR-α, a regulator of β-oxidation, in rats that were fed a HFD (83,84,94,95,101,105,109,114,115). This suggests that AMPK activation by these herbal compounds is associated with de novo lipid synthesis, which is linked to the suppression of SREBP-1c, PPAR-α, FAS, ACC and CPT-2 expression.…”

Section: Mechanism Of Actionsmentioning

confidence: 87%

“…The extracts of Antidesma bunius (82,83), Aralia elata (84)(85)(86), Citrus aurantium (90)(91)(92)(93), Curcuma longa Linn. (94)(95)(96), Cyclosorus terminans (101,102), Panax notoginseng (109), Pluchea indica (114), and Rosmarinus of f icinalis Linn (115,116) have also been shown to reduce the accumulation of lipids in rats fed a HFD through sterol regulatory element-binding transcription factor 1c (SREBP-1c), peroxisome proliferator-activated receptor-α (PPAR-α), fatty acid synthase (FAS), acetyl-CoA carboxylase (ACC), and carnitine palmitoyltransferase (CPT)2 regulation. The adenosine monophosphate-activated protein kinase (AMPK) pathway plays a crucial role in regulating hepatic lipogenesis and β-oxidation and serves as a vital energy sensor for intracellular energy metabolism.…”

Section: Mechanisms Of Actionmentioning

confidence: 99%

Mechanistic pathway of herbs in the amelioration of NAFLD: A systematic review

Jayanti,

Said,

Jofrry

et al. 2024

World Acad Sci J

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Non-alcoholic fatty liver disease (NAFLD) is identified by lipid accumulation in the liver and liver injury due to obesity and metabolic syndrome. Herbs have been used in the treatment of various diseases, including liver disease. The present systematic review aimed to identify plants and the doses used in the management and prevention of NAFLD, as well as to assess the safety of these plants and determine their mechanisms of action, providing the preclinical evidence-based usage of herbs. Scientific databases, namely, Scopus, PubMed, Springer, NCBI, Google Scholar, Science Direct, and Web of Science were searched with key words, such as 'non-alcoholic fatty liver disease', 'non-alcoholic steatohepatitis', 'metabolic-associated fatty liver disease', 'medicinal plants', 'hyperlipidemia' and 'plant extracts' from January, 2016 to November, 2023. Manual screening, quality assessment, and data extraction of the search results were performed according to the inclusion and exclusion criteria. Herbs were identified which were able to ameliorate NAFLD symptoms in rodents through lipid metabolism, insulin resistance, inflammation and oxidative stress. These herbs were identified to lead to a reduction in steatosis in the histopathological assessment. The acute or chronic toxicity studies were not found to indicate any signs of toxicity. The present study provides information on the highest dose evaluated and which was effective against NAFLD, with safety assessment in studies using rats. Since chronic liver diseases progress over a long period of time with minimal or no symptoms, the consumption of herbs may provide alternative treatment strategies for the prevention of NAFLD. Further studies are warranted however, to identify their bioactive compounds for drug development or for the standardization of crude extracts. Drug-herb interactions also need to be further evaluated when used concurrently with drugs.

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“…A positive energy balance coming from a high energy intake promotes excessive accumulation of visceral fat and obesity-related comorbidities [ 44 , 45 ]. As expected, the H group showed obesity (↑body weight gain and ↑body fat accumulation by MRI), as reported in high-fat/high-fructose diet models [ 45 ]. Meanwhile, HBL prevented obesity (↓body fat accumulation by MRI without changes in body weight gain).…”

Section: Discussionmentioning

confidence: 99%

“…Hence, the H group developed obesity and dyslipidemia by increased total cholesterol, triglycerides, VLDL-c, and HDL-c, in addition to impaired glucose tolerance, compensatory hyperinsulinemia, and insulin resistance. These common metabolic alterations induced by high-fat/high-fructose models are strongly related to nonalcoholic liver disease development [ 45 ].…”

Section: Discussionmentioning

confidence: 99%

Bean Leaves Ameliorate Lipotoxicity in Fatty Liver Disease

et al. 2023

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Bioactive compounds in plant-based food have protective effects against metabolic alterations, including non-alcoholic fatty liver disease (NAFLD). Bean leaves are widely cultivated in the world and are a source of dietary fiber and polyphenols. High fat/high fructose diet animal models promote deleterious effects in adipose and non-adipose tissues (lipotoxicity), leading to obesity and its comorbidities. Short-term supplementation of bean leaves exhibited anti-diabetic, anti-hyperlipidemic, and anti-obesity effects in high-fat/high-fructose diet animal models. This study aimed to evaluate the effect of bean leaves supplementation in the prevention of lipotoxicity in NAFLD and contribute to elucidating the possible mechanism involved for a longer period of time. During thirteen weeks, male Wistar rats (n = 9/group) were fed with: (1) S: Rodent Laboratory Chow 5001® (RLC); (2) SBL: 90% RLC+ 10% dry bean leaves; (3) H: high-fat/high-fructose diet; (4) HBL: H+ 10% of dry bean leaves. Overall, a HBL diet enhanced impaired glucose tolerance and ameliorated obesity, risk factors in NAFLD development. Additionally, bean leaves exerted antioxidant (↑serum GSH) and anti-inflammatory (↓mRNA TNFα in the liver) effects, prevented hepatic fat accumulation by enhanced ↑mRNA PPARα (β oxidation), and enhanced lipid peroxidation (↓liver MDA). These findings suggest that bean leaves ameliorated hepatic lipotoxicity derived from the consumption of a deleterious diet.

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Cyclosorus terminans Extract Ameliorates Insulin Resistance and Non-Alcoholic Fatty Liver Disease (NAFLD) in High-Fat Diet (HFD)-Induced Obese Rats

Cited by 9 publications

References 65 publications

Beta-Caryophyllene Modifies Intracellular Lipid Composition in a Cell Model of Hepatic Steatosis by Acting through CB2 and PPAR Receptors

Beta-Caryophyllene Modifies Intracellular Lipid Composition in a Cell Model of Hepatic Steatosis by Acting through CB2 and PPAR Receptors

Mechanistic pathway of herbs in the amelioration of NAFLD: A systematic review

Bean Leaves Ameliorate Lipotoxicity in Fatty Liver Disease

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