Cyclosporin‐Induced Downregulation of the Expression of E‐Cadherin During Proliferation of Edentulous Gingival Epithelium in Rats

Abstract

CsA treatment reduced the production of E-cadherin but increased the production of beta-catenin, Cyclin D1, and PCNA. Thus, CsA may downregulate E-cadherin gene expression, leading to the epithelial cell proliferation of gingival overgrowth.

Cyclosporine A enhances apoptosis in gingival keratinocytes of rats and in OECM1 cells via the mitochondrial pathway

“…Therefore, we suggest that cyclosporine A can enhance cell apoptosis, via the Bax‐mediated mitochondrial pathway, in rat gingival keratinocytes and in OECM1 cells. The enhanced apoptosis in epithelia, in combination with the observed epithelial proliferation in our previous studies (6,47), might also suggest a rapid turnover of keratinocytes in gingiva during treatment with cyclosporine A.…”

Cyclosporine A up‐regulates Sonic hedgehog in gingiva: role of the up‐regulation on gingival cell proliferation

“…SHH‐expressing cells fail to exit S and G2/M phases and persist in the cycle ; this induction of cell proliferation is accomplished by up‐regulation of cyclin D1 and cyclin E, which promote pRB and G1 to S transition . In our previous studies , gingival expression of cyclin D1 and pRB, which are SHH‐related genes/mediators, showed a significant increase after treatment with cyclosporine A. We thus speculated that cyclosporine A might up‐regulate the expression of Shh and further modulate the proliferation of gingival cells.…”

“…In our previous studies, increased production of SHH target genes and related mediators [e.g. b-catenin, cyclin D1, cyclin E, PCNA and transforming growth factor-b (TGF-b)] and reduced production of E-cadherin were observed after treatment with cyclosporine A (7,8,23). The effects of cyclosporine A on the associated proteins (e.g.…”

Crosstalk Between Human Monocytic U937 Cells and Gingival Fibroblasts in Coculturally Enhanced Matrix Metalloproteinase‐2 Expression

“…Among all MMPs, MMP‐2 is known to participate in periodontal destruction, and expression of MMP‐2 increases in gingiva of patients with periodontitis 8–12 . Recent studies by the authors 13 , 14 also showed MMP‐2 enzyme level synergistically enhances in a coculture system of human gingival fibroblasts (HGFs) and U937 cells, compared with levels in an independent HGF or U937 cell culture. However, it remains unclear whether enhanced MMP‐2 expression in the coculture is regulated by interaction between HGFs and U937 cells.…”