CYFIP2, a Direct p53 Target, is Leptomycin-B Sensitive

Killin is a p53-regulated nuclear inhibitor of DNA synthesis

“…DNA sequence analysis revealed that four of them, G20, G54, G63, and G116, corresponded to the wild-type p53 transgene itself (SI Text and SI Table 1). Among these were also several known bona fide p53 target genes, including the human homolog of mdm-2 (found twice, A10 and G10) and p21 (A21), whereas the rest of the candidate p53 target genes, including G101 (killin), NDRG1 (22), CYFIP2 (23), and Tis11D (24), were either novel genes or novel p53 targets. Our findings of p53 induction, and other major known p53 target genes, several times by FDD demonstrated excellent gene coverage and accuracy of our FDD platform, because the method is nonbiased and does not require prior knowledge of gene sequences detected (20).…”

Ebf1orPax5haploinsufficiency synergizes with STAT5 activation to initiate acute lymphoblastic leukemia

“…However, 25% of EBF1-induced genes were down-regulated, suggesting that they were sensitive to small perturbations in Ebf1 expression (Table S3). These include several potential tumor suppressor genes including Bcl7a, Cyfip2, and Rgs2 (Zani et al, 1996;Jackson et al, 2007;Schwäble et al, 2005;van Doorn et al, 2005). Likewise, 25% of PAX5-induced genes were also downregulated in Stat5b-CA x Ebf1 +/ leukemias, including potential tumor suppressor genes or genes involved in suppressing cell proliferation such as Bach2, Btg1, Btg2, and Ucp2 (Table S3; Rouault et al, 1992Rouault et al, , 1996Sasaki et al, 2000;Lim, 2006;Baffy, 2010).…”

Cell cycle specific distribution of killin: evidence for negative regulation of both DNA and RNA synthesis

“…4 Many p53 target genes play direct roles in p53-dependent apoptosis including the death receptor, Fas; the pro-apoptotic Bcl ¡ 2 family members, Bax, Noxa, and Puma; proteins important for p53 hyper-activation, p53AIP1 and p53DINP1; the zinc-finger protein, PAG608; the KH-RNA binding domain containing protein, MCG10; PIG3, Pidd, p53RDL1, mRTVP-1, mtCLIC/ CLIC4, PAC1, and NDRG1. 8,[14][15][16][17] In fact majority of these genes were discovered by Differential Display technology that we pioneered, yet the complete network of genes responsible for mediating p53-dependent apoptosis remains unclear.…”

“…[14][15][16][17] killin is encoded by a single exon located on human chromosome 10 within 140 bp from another major tumor suppressor gene, pTEN. 17 The 140 bp intergenic region contains a divergent promoter which drives the p53-dependent expression of killin and largely constitutive expression of pTEN.…”