Cysteine Protease Inhibitor from Pearl Millet: A New Class of Antifungal Protein

Joshi, Bimba N.; Sainani, Mohini N.; Bastawade, Kulbhushan B.; Gupta, Vidya S.; Ranjekar, P. K.

doi:10.1006/bbrc.1998.8625

Cited by 158 publications

(84 citation statements)

References 29 publications

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“…However, A. niger was more susceptible, since it required a lesser AhCPI concentration to inhibit spore germination compared with A. parasiticus . Accordingly, it has been suggested that the cystatin inhibitory effect is determined by the fungal species (Porruan et al 2013); this hypothesis is in agreement with this study and others (Joshi et al 1998; Pernas et al 2000; Martínez et al 2003; Valdés-Rodríguez et al 2010). These results may be explained in terms of cell wall organization and composition, which are extremely variable and depend on many factors such as environmental changes, developmental stage, fungal species, etc.…”

Section: Resultssupporting

confidence: 92%

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In vitroeffect of recombinant amaranth cystatin (AhCPI) on spore germination, mycelial growth, stress response and cellular integrity ofAspergillus nigerandAspergillus parasiticus

et al. 2015

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The inhibitory effect of recombinant amaranth cystatin (AhCPI) on the spore germination and growth of the mycotoxigenic fungus Aspergillus parasiticus and Aspergillus niger was investigated. AhCPI showed a concentration-dependent antifungal activity against both fungi. Differential effects were observed when fungi were treated with cystatin in two developmental stages. When AhCPI was added to young mycelium cultures of A. niger, it had a dramatic effect on mycelial growth compared with old mycelium cultures. On the contrary, there was no differential effect of AhCPI addition to either old or young mycelium of A. parasiticus. Furthermore, electron microscopic observations showed that cystatin caused important effects at the level of cell morphology and organelle integrity of both fungi. Additionally, A. parasiticus spores treated with AhCPI presented sensitivity to oxidative, osmotic and ionic stresses; in opposition, under same conditions, A. niger did not show sensitivity to any stressful agent. These results suggest that AhCPI antifungal activity might be related with damage to cell integrity, affecting the survival of the fungi. In addition, our evidences showed that fungal species respond dissimilarly to cystatin; however, such disparities can be used to the control of unwanted fungi.

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Section: Resultssupporting

confidence: 92%

“…Cystatin from pearl millet ( Pennisetum glaucum ) inhibited the mycelial growth of Claviceps paspali, Claviceps purpurea, Curvularia fallax, Curvularia cymbopogonis, Curvularia lunata , as well as Alternaria solani and Fusarium oxysporum (Joshi et al 1998). Canecystatin affected spore germination of Trichoderma reesei (Soares-Costa et al 2002).…”

Section: Resultsmentioning

confidence: 99%

In vitroeffect of recombinant amaranth cystatin (AhCPI) on spore germination, mycelial growth, stress response and cellular integrity ofAspergillus nigerandAspergillus parasiticus

et al. 2015

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“…Protein inhibitors of serine (e.g., trypsin and chymotrypsin) and cysteine proteases have emerged as a class of antifungal proteins that have potent activity against plant and animal pathogens. Cysteine protease inhibitors have been isolated from a number of plants and form a fourth group of cystatins, the phytocystatins (10,72,86,139). The phytocystatins are single polypeptides of 10 to 12 kDa and share common structural motifs.…”

Section: Killer Proteins (Killer Toxins)mentioning

confidence: 99%

Antifungal Proteins

Selitrennikoff

2001

Appl Environ Microbiol

529

362

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“…In different plants two well-known subclasses of these AMPs are found which are called as thionins and plant defensins [148]. Chitin-binding proteins [146], knottin-type peptides [149] and protease inhibitors [150] are some other AMPs which have also been isolated from medicinal plants and studied. Much attention has been given in recent years to the potential use of AMPs to design novel fungicides which should be environmentally friendly.…”

Section: Bioactive Compounds and Defense Mechanisms In Plantsmentioning

confidence: 99%

Bioactive Compounds from Medicinal Plants and Their Importance in Drug Discovery in Pakistan

Mustafa¹,

Arif²,

Atta³

et al. 2017

Matrix sci. pharma

126

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Humans have relied on nature throughout their ages to cater for their basic needs including medicines to cure a wide spectrum of diseases. Plants have formed the basis for sophisticated systems of traditional medicines. For therapeutic agents many of the presently known lead compounds are natural products or their derivatives. Ethnomedicinal studies play a vital role to discover new drugs from indigenous medicinal plants. Green pharmaceuticals are getting popularity and extraordinary importance because vast opportunities for new drug discoveries are provided by the unmatched availability of chemical diversity and natural products either as pure compounds or as homogenous plant extracts. Therefore, in recent years the demand for herbal medicines and several natural products from a variety of plant species is consistently increasing. In spite of being an agricultural country and having different ecological regions, the medicinal plants of Pakistan have not been explored for their secondary metabolites which are responsible for treating different diseases. Although, huge importance of different extracts of medicinal plants from Pakistan have been reported for their different activities such as antimicrobial, anti-cancerouse, antiviral and antioxidant but complete biochemical profiling of these medicinal plants is lacking. LC-MS and GC-MS techniques have been applied in the field of drug discovery from medicinal plants but in Pakistan its success rate is very low in the subject of biochemical profiling. Therefore, such techniques should be used in Pakistan to explore active constituents from medicinal plants which could be used as medicines in future.

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Cysteine Protease Inhibitor from Pearl Millet: A New Class of Antifungal Protein

Cited by 158 publications

References 29 publications

In vitroeffect of recombinant amaranth cystatin (AhCPI) on spore germination, mycelial growth, stress response and cellular integrity ofAspergillus nigerandAspergillus parasiticus

In vitroeffect of recombinant amaranth cystatin (AhCPI) on spore germination, mycelial growth, stress response and cellular integrity ofAspergillus nigerandAspergillus parasiticus

Antifungal Proteins

Bioactive Compounds from Medicinal Plants and Their Importance in Drug Discovery in Pakistan

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