Cystic Fibrosis: Isolation and Physical Properties of a Salivary Cystic Fibrosis Factor

Specificity of an Isolated Salivary Factor Material to Cystic Fibrosis

“…This enzymatic method utilizes the finding that the purified factor inhibits mammalian glycogen debranching enzyme (12). The enzymatic method is rapid, quantitative, can be used on a routine basis, and avoids the uncertainties inherent in bioassays (12,15). The noncompetitive type of inhibition produced by the isolated factor material on the activity of the debranching enzyme gives an indication of the chemical nature of the factor as has been discussed (15).…”

“…The CF factor material isolated by the glycogen method is a potent ciliostatic agent (15). The activity of the isolated factor material is proportional to its concentration in both the oyster gill ciliostatic assay and the debranching enzyme inhibition assay (15). The factor material when isolated from the amylase is in the form of an apparent factor-peptide complex (17)(18)(19).…”

“…The amylase is purified from whole CF saliva by a glycogen-complex method, and the factor is then separated from the amylase by dialysis (15). The CF factor material isolated by the glycogen method is a potent ciliostatic agent (15). The activity of the isolated factor material is proportional to its concentration in both the oyster gill ciliostatic assay and the debranching enzyme inhibition assay (15).…”

“…Using the enzyme inhibition assay (12), a method for the isolation and purification of a ciliostatic CF factor from whole saliva has been developed (15). The isolation method utilizes the fact that the factor material forms a complex with a-amylase in saliva and remains bound to the amylase through subsequent purification of the enzyme.…”

“…The isolation method utilizes the fact that the factor material forms a complex with a-amylase in saliva and remains bound to the amylase through subsequent purification of the enzyme. The amylase is purified from whole CF saliva by a glycogen-complex method, and the factor is then separated from the amylase by dialysis (15). The CF factor material isolated by the glycogen method is a potent ciliostatic agent (15).…”