Cytidine-phosphate-guanosine oligodeoxynucleotides in combination with CD40 ligand decrease periodontal inflammation and alveolar bone loss in a TLR9-independent manner

Zhao, Qian; Hu, Yang; Deng, Shu; Yu, Pei; Chen, Bowen; Wang, Zuomin; Han, Xiaozhe

doi:10.1590/1678-7757-2017-0451

Cited by 9 publications

(11 citation statements)

References 30 publications

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“…286 Timely reports have characterized regulatory B cells to play a critical role in suppressing inflammatory responses in vitro 292,293 and ameliorating alveolar bone loss in vivo. [293][294][295] Liu et al performed an in vitro study in which isolated splenic B cells were immunized with P. gingivalis and treated with CD40 ligand and CpG to induce B cell-derived toll-like receptor signaling. 292 Immunized B cells secreted elevated interleukin-10 levels, compared with nonimmunized B cells.…”

Section: B Cellsmentioning

confidence: 99%

“…294,296 Zhao et al found that CD40 ligand and CpG induced regulatory B cells ameliorate ligature-induced periodontitis in a toll-like receptor-9-independent manner. 295 Similarly, Shi et al adoptively transferred periodontopathogenic-specific regulatory B cells, which alleviated alveolar bone loss, increased interleukin-10 production, and reduced interleukin-17 and T H 17 cells in the murine gingiva. 297 Advancing knowledge about B cell subsets and their diverse roles in periodontal health and disease will provide opportunity for the development of therapeutic targets in the B cell lineage for the treatment of periodontitis.…”

Section: B Cellsmentioning

confidence: 99%

“…In contrast, Demoersman et al reported that B1 cells are diminished in peripheral blood of periodontitis patients compared with healthy patients 286 . Timely reports have characterized regulatory B cells to play a critical role in suppressing inflammatory responses in vitro 292,293 and ameliorating alveolar bone loss in vivo 293‐295 . Liu et al performed an in vitro study in which isolated splenic B cells were immunized with P. gingivalis and treated with CD40 ligand and CpG to induce B cell–derived toll‐like receptor signaling 292 .…”

Section: Osteoimmunology: Interaction Of Immune Cells: Bone Cellsmentioning

confidence: 99%

“…CD40 ligand and CpG–induced regulatory B cells alleviated periodontal bone loss 294,296 . Zhao et al found that CD40 ligand and CpG induced regulatory B cells ameliorate ligature‐induced periodontitis in a toll‐like receptor‐9–independent manner 295 . Similarly, Shi et al adoptively transferred periodontopathogenic‐specific regulatory B cells, which alleviated alveolar bone loss, increased interleukin‐10 production, and reduced interleukin‐17 and T H 17 cells in the murine gingiva 297 …”

Section: Osteoimmunology: Interaction Of Immune Cells: Bone Cellsmentioning

confidence: 99%

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Maintaining homeostatic control of periodontal bone tissue

Hathaway‐Schrader

Novince

2021

Periodontology 2000

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Alveolar bone is a unique osseous tissue due to the integration of the teeth and the proximity of dental plaque biofilms. Periodontal health and homeostasis are mediated by a balanced host immune response to polymicrobial oral biofilms. Shifts in the composition and quantity of microbes within dental plaque biofilms can drive a local proinflammatory immune response state in the epithelial and gingival barrier connective tissues. Infiltrating proinflammatory immune cells within the inflamed connective tissue secrete local factors that induce paracrine signaling to subjacent bone cells.Sustained chronic inflammation disrupts "coupled" osteoclast-osteoblast actions, which ultimately result in alveolar bone destruction. This chapter will provide an overview of alveolar bone physiology and will highlight why the oral microbiota is a critical regulator of alveolar bone remodeling. The ecology of subgingival plaque biofilms will be reviewed considering our understanding that periodontitis is a polymicrobial disruption of host homeostasis. The pathogenesis of periodontal bone loss will be explained from both a historical and current perspective, providing the opportunity to revisit the role of fibrosis in alveolar bone destruction. The molecular basis of host-microbe interactions will be discussed in the context of the commensal oral flora in periodontal health and pathogenic shifts in the oral microbiota during periodontal disease states. The role of periodontal innate and adaptive immune cell interactions with bone cells will be reviewed based on our current understanding of osteoimmunological mechanisms influencing alveolar bone remodeling. Lastly, probiotic/prebiotic therapeutic interventions in the oral microbiota will be evaluated as a potential therapy to support alveolar bone homeostasis/ prevent periodontal bone loss.

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Section: B Cellsmentioning

confidence: 99%

Section: B Cellsmentioning

confidence: 99%

Section: Osteoimmunology: Interaction Of Immune Cells: Bone Cellsmentioning

confidence: 99%

Section: Osteoimmunology: Interaction Of Immune Cells: Bone Cellsmentioning

confidence: 99%

See 2 more Smart Citations

Maintaining homeostatic control of periodontal bone tissue

Hathaway‐Schrader

Novince

2021

Periodontology 2000

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“…Recent studies have shown that certain specific oligodeoxynucleotide (ODN) sequences can inhibit inflammation and reduce alveolar bone resorption. 5,6 ODN can be classified into stimulatory ODN, inhibitory ODN, and inert ODN. Stimulatory ODN generally refers to CpG ODN containing a CG motif.…”

Section: Introductionmentioning

confidence: 99%

<p><em>N</em>-Acetyl-l-Leucine-Polyethyleneimine-Mediated Delivery of CpG Oligodeoxynucleotides 2006 Inhibits RAW264.7 Cell Osteoclastogenesis</p>

Wang

et al. 2020

DDDT

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Introduction: CpG oligodeoxynucleotides (CpG ODN) play important roles in resisting inflammation and bone resorption. However, the inherent instability and rapid degradation hinder their wider application. This study aimed to evaluate whether N-acetyl-L-leucinemodified polyethyleneimine (N-Ac-L-Leu-PEI) could effectively deliver CpG ODN 2006 to RAW264.7 cells and and if it can regulate osteoclastogenesis in vitro. Materials and Methods: Gel retardation assay was conducted to evaluate whether N-Ac-L-Leu-PEI and CpG ODN could form a stable complex. RAW264.7 cells were divided into four groups of control group, ODN group, phosphorothioate ODN group and N-Ac-L-Leu-PEI/ODN group. Fluorescence assay was conducted to evaluate the transfection rate of ODNs in different groups. Cell viability was determined by MTT assay. Cell apoptosis was determined by live-dead cell staining and flow cytometry assay. Relative expression levels of osteoclastic differentiation factors, including Nfatc, c-fos, receptor activator of nuclear factor κB (RANK), and matrix metalloproteinase 9 (MMP9), were determined by real-time PCR and Western blot. Results: N-Ac-L-Leu-PEI and CpG ODN could form a stable complex at a mass ratio of 1:1 (w:w). MTT assay showed that the cell viability of N-Ac-L-Leu-PEI was relatively high even at a mass ratio of 8 μg/mL. The transfection rate of N-Ac-L-Leu-PEI-ODN complex was higher than 90%. The cell proliferation and apoptosis was significantly enhanced in N-Ac-L-Leu-PEI-CpG ODN group when compared to those in phosphorothioate CpG ODN. The expression levels of Nfatc, c-fos, RANK, and MMP9 were significantly decreased in N-Ac-L-Leu-PEI/ODN complex group. Discussion: N-Ac-L-Leu-PEI could be a potential gene vehicle for the prevention of periodontitis-mediated bone resorption.

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A simplified direct on‐chip forward or reverse immunoassay for evaluating protein–protein interactions in the serum

Liu

et al. 2022

Biotechnology Journal

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Background:The identification of protein-protein interactions is a great challenge. In this study, we fabricated a gold surface-modified biochip with activated sophorolipids (SLs) in combination with 16-amino-1-hexadecanethiol hydrochloride to detect serum proteins. Main Methods and Major Results:The on-chip immunoassay reported here included a forward assay, in which a ligand is immobilized on the biochip surface and allowed to interact with its free specific receptor in liquid phase, and a reverse assay, in which a receptor is loaded on the biochip surface and combined with its free specific ligand in solution. The specificity of the molecular interactions on the biochip was evaluated using immunological blocking assays and chemiluminescent immunoassays (CLIA).Hemophagocytic lymphohistiocytosis (HLH) serum was used to test the potential utilization of the biochip. Reverse receptor CD25-based interleukin (IL)-2 and forward ligand IL-2-based CD25 assays revealed that the limit of detection of the target proteins was as low as 156 and 78 pg/ml, respectively. Using receptor-or ligand-based platforms, we found that the positive rates of free IL-2 and soluble CD25 (sCD25) monomers in the sera of HLH patients were 14.3% and 71.4%, respectively. In addition, the biochip showed good compatibility with CLIA for the measurement of sCD25 (r = 0.77, p < 0.01). Conclusions and Implications:Biochip platforms, such as on-chip immunoprecipitation (IP), can be used to evaluate the interactions between proteins, ligands, and receptors, or enzymes and substrates in serum.

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Cytidine-phosphate-guanosine oligodeoxynucleotides in combination with CD40 ligand decrease periodontal inflammation and alveolar bone loss in a TLR9-independent manner

Cited by 9 publications

References 30 publications

Maintaining homeostatic control of periodontal bone tissue

Maintaining homeostatic control of periodontal bone tissue

<p><em>N</em>-Acetyl-l-Leucine-Polyethyleneimine-Mediated Delivery of CpG Oligodeoxynucleotides 2006 Inhibits RAW264.7 Cell Osteoclastogenesis</p>

A simplified direct on‐chip forward or reverse immunoassay for evaluating protein–protein interactions in the serum

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