Cytochalasans and azaphilones: suitable chemotaxonomic markers for the Chaetomium species

Yang, Mu; Chang, Qing-Hua; Li, Longfei; Liu, Yunfeng; Cao, Fei

doi:10.1007/s00253-021-11630-2

Cited by 11 publications

(4 citation statements)

References 114 publications

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“…Azaphilones are synthesized by fungi spanning 61 genera, including Aspergillus, Penicillium, Chaetomium, Talaromyces, Pestalotiopsis, Phomopsis, Emericella, Epicoccum, Monascus and Hypoxylon [44,47]. Over time, chemical analyses of fungal azaphilones and the exploration of their metabolic pathways have supported the idea that these compounds are predominantly genus-or even species-specific [42,46,48,49], to the extent that azaphilones have been proposed as markers for taxonomic purposes in certain fungi [50][51][52]. The findings presented in this work suggest that Pseudogymnoascus is yet another fungal genus capable of azaphilone production.…”

Section: Discussionmentioning

confidence: 99%

Expanding the Toolbox for Genetic Manipulation in Pseudogymnoascus: RNAi-Mediated Silencing and CRISPR/Cas9-Mediated Disruption of a Polyketide Synthase Gene Involved in Red Pigment Production in P. verrucosus

Palma,

Oliva,

Montanares

et al. 2024

JoF

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Fungi belonging to the genus Pseudogymnoascus have garnered increasing attention in recent years. One of the members of the genus, P. destructans, has been identified as the causal agent of a severe bat disease. Simultaneously, the knowledge of Pseudogymnoascus species has expanded, in parallel with the increased availability of genome sequences. Moreover, Pseudogymnoascus exhibits great potential as a producer of specialized metabolites, displaying a diverse array of biological activities. Despite these significant advancements, the genetic landscape of Pseudogymnoascus remains largely unexplored due to the scarcity of suitable molecular tools for genetic manipulation. In this study, we successfully implemented RNAi-mediated gene silencing and CRISPR/Cas9-mediated disruption in Pseudogymnoascus, using an Antarctic strain of Pseudogymnoascus verrucosus as a model. Both methods were applied to target azpA, a gene involved in red pigment biosynthesis. Silencing of the azpA gene to levels of 90% or higher eliminated red pigment production, resulting in transformants exhibiting a white phenotype. On the other hand, the CRISPR/Cas9 system led to a high percentage (73%) of transformants with a one-nucleotide insertion, thereby inactivating azpA and abolishing red pigment production, resulting in a white phenotype. The successful application of RNAi-mediated gene silencing and CRISPR/Cas9-mediated disruption represents a significant advancement in Pseudogymnoascus research, opening avenues for comprehensive functional genetic investigations within this underexplored fungal genus.

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Section: Discussionmentioning

confidence: 99%

Expanding the Toolbox for Genetic Manipulation in Pseudogymnoascus: RNAi-Mediated Silencing and CRISPR/Cas9-Mediated Disruption of a Polyketide Synthase Gene Involved in Red Pigment Production in P. verrucosus

Palma,

Oliva,

Montanares

et al. 2024

JoF

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“…To explore new Hsp expression inhibitors we selected azaphilones, secondary metabolites of fungi. Azaphilones have been isolated from Penicillium [ 12 ], Aspergillus [ 8 ], Chaetomium [ 13 ], Talaromyces [ 14 ], and Muyocopron [ 15 ] species. Among these fungi, more than 50 azaphilones were reported from the species of Penicillium section Sclerotiora [ 16 – 19 ] proving this species is the major source of azaphilones.…”

Section: Introductionmentioning

confidence: 99%

Azaphilones produced by Penicillium maximae with their cell death-inducing activity on Adriamycin-treated cancer cell

et al. 2023

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Background Heat shock proteins (Hsps) are overexpressed in several tumors and contribute to cell proliferation, metastasis, and anticancer drug resistance. Therefore, Hsp inhibitors have enhanced cytotoxicity as chemotherapeutic agents and may be effective with a reduced dosage for tumor therapy to avoid side effects. Results Four new azaphilones, maximazaphilones I–IV (1–4), and three known compounds (5–7) have been isolated from the airborne-derived fungus Penicillium maximae. Inhibitory effects of isolated compounds against induction of Hsp105 were evaluated by the luciferase assay system using Hsp105 promoter. In this assay, 2–4, 6, and 7 significantly inhibited hsp105 promoter activity without cytotoxicity. In addition, all isolated compounds except for 5 significantly induced the death of Adriamycin (ADR)-treated HeLa cells. Interestingly, 1–4, 6, and 7 didn’t show anti-proliferative and cell death-inducing activity without ADR. Conclusion This study revealed the chemical structures of maximazaphilones I–IV (1–4) and the potency of azaphilones may be useful for cancer treatment and reducing the dose of anticancer agents. In addition, one of the mechanisms of cell death-inducing activity for 2–4, 6, and 7 was suggested to be inhibitory effects of Hsp105 expression.

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“…Endophytes are microorganisms that spend part or whole of their life cycles inside the healthy tissues of host plants, typically causing no apparent symptoms of diseases (Debbab et al 2011 ; Kaul et al 2012 ; Wang et al 2017a ; Ancheeva et al 2020 ). In the symbiotic relationship, the endophytes are fed and protected by the host plants, and in return, the microorganisms produce bioactive secondary metabolites involving in the growth of the host plant and protecting the plants from pathogens and herbivores (Abdel-Azeem et al 2016 ; Yang et al 2021 ; Zhang et al 2021 ; Collinge et al 2022 ). The fungi, one major category of the endophytes, have been reported to be capable of producing a diverse array of specialized metabolites, and can produce chemical entities that are also present in plants or have similar biological activities as the compounds from plant sources (Mishra et al 2022 ; Wen et al 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Chemical constituents from the medicinal herb-derived fungus Chaetomium globosum Km1226

Chang,

Hsiao,

Wang

et al. 2023

Bot Stud

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Background Endophytic fungi have proven to be a rich source of novel natural products with a wide-array of biological activities and higher levels of structural diversity. Results Chemical investigation on the liquid- and solid-state fermented products of Chaetomium globosum Km1226 isolated from the littoral medicinal herb Atriplex maximowicziana Makino resulted in the isolation of compounds 1–14. Their structures were determined by spectroscopic analysis as three previously undescribed C13-polyketides, namely aureonitol C (1), mollipilins G (2), and H (3), along with eleven known compounds 4–14. Among these, mollipilin A (5) exhibited significant nitric oxide production inhibitory activity in LPS-induced BV-2 microglial cells with an IC50 value of 0.7 ± 0.1 µM, and chaetoglobosin D (10) displayed potent anti-angiogenesis property in human endothelial progenitor cells (EPCs) with an IC50 value of 0.8 ± 0.3 µM. Conclusions Three previously unreported compounds 1–3 were isolated and identified. Mollipilin A (5) and chaetoglobosin D (10) could possibly be developed as anti-inflammatory and anti-angiogenic lead drugs, respectively.

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Cytochalasans and azaphilones: suitable chemotaxonomic markers for the Chaetomium species

Cited by 11 publications

References 114 publications

Expanding the Toolbox for Genetic Manipulation in Pseudogymnoascus: RNAi-Mediated Silencing and CRISPR/Cas9-Mediated Disruption of a Polyketide Synthase Gene Involved in Red Pigment Production in P. verrucosus

Expanding the Toolbox for Genetic Manipulation in Pseudogymnoascus: RNAi-Mediated Silencing and CRISPR/Cas9-Mediated Disruption of a Polyketide Synthase Gene Involved in Red Pigment Production in P. verrucosus

Azaphilones produced by Penicillium maximae with their cell death-inducing activity on Adriamycin-treated cancer cell

Chemical constituents from the medicinal herb-derived fungus Chaetomium globosum Km1226

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