Cytochalasin B and the Adenosine Triphosphate Content of Treated Fibroblasts

Warner, David A.; Perdue, James F.

doi:10.1083/jcb.55.1.242

Cited by 26 publications

(11 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, even in glucose-free medium, the inhibitors induced less than maximal rounding when contrasted to the anesthetics. It is also of interest that levels of ATP were unchanged in fibroblasts that contracted by exposure to cytochalasin B (42).…”

Section: Discussionmentioning

confidence: 99%

Cell shape changes induced by cationic anesthetics.

Rabinovitch¹,

DeStefano²

1976

The Journal of Experimental Medicine

View full text Add to dashboard Cite

Cell functions such as adhesion, movement, and phagocytosis involve changes in both cell shape and cell surface motility. These changes are the result of membrane perturbations caused by interactions of cells with substrates, particles, or other cells. Surface perturbations generate signals that are presumably transduced into changes in the state of aggregation and distribution of submembranous cytoskeletal constituents (1-3). Except for the role of Fc and C3 receptors in the interaction of particles or substrates with phagocytes (3), other cell surface-"sensing" components, as well as the nature of the membrane perturbations and the transducing mechanisms are unknown. Induced cell shape changes permit the analysis of factors involved in maintenance of cell shape and in cell motility. A few diverse agents promote rapid and reversible changes in cell shape. Examples are the spreading of cells induced by Mn 2÷ (4) and the cell rounding induced by high hydrostatic pressure or exposure to trypsin, strychnine, EDTA, or cytochalasins (5-9). Cationic anesthetics inhibit a variety of cell surface phenomena, including cell to substrate adhesion, spreading, phagocytesis, locomotion, cell fusion, capping of surface Ig, or cell mediated-cytotoxicity (10-16). We show here that exposure of cultivated macrophages to cationic anesthetics results in extensive and fully reversible contraction and rounding. Single cells were followed before, during, and after exposure to the drugs and the observations recorded in still pictures and time-lapse movies. We examined several features of anesthetic-induced rounding, investigated structure-activity relationships of the drugs, and compared anesthetic effects to those of EDTA. Materials and Methods

show abstract

Section: Discussionmentioning

confidence: 99%

Cell shape changes induced by cationic anesthetics.

Rabinovitch¹,

DeStefano²

1976

The Journal of Experimental Medicine

View full text Add to dashboard Cite

show abstract

“…It is agreed that phosphorylation of hexose is not disturbed by cytochalasin (23,32,50,62) . Neither cell membrane ATPase activity, nor the cellular contents of ATP or cyclic AMP, are reportedly influenced by CB at least up to 4 h of exposure (50,97) .…”

Section: Permeabilitymentioning

confidence: 99%

Action of Cytochalasin D on Cells of Established Lines

Miranda

Godman

Deitch

et al. 1974

The Journal of Cell Biology

216

119

View full text Add to dashboard Cite

HeLa, Vero, L, HEp2, and MDBK cells respond immediately to 0.2–0.5 µg/ml cytochalasin D (CD) with sustained contraction (contracture), loss of microvilli, expression of endoplasmic contents (zeiosis), nuclear protrusion, and extension of cytoplasmic processes. The development of these changes is depicted, and the dose-response patterns in these cell lines are described. MDBK is generally most resistant and HeLa most sensitive to these effects of CD. Cells in G1 are most sensitive to CD; responsiveness decreases progressively during early S and is least in mid S through G2. CD inhibits transport of [14C]deoxyglucose in HeLa by about 45% but has no significant effect on hexose uptake in Vero and MDBK; sugar transport is thus apparently unrelated to any morphologic effect of CD. Although spreading and attachment are impeded, CD does not decrease and may even enhance the adhesiveness of established monolayers. Contraction appears to be a primary early effect of CD, upon which other visible changes follow. It is prevented by some inhibitors of energy metabolism (deoxyglucose and dinitrophenol) and does not occur in glycerinated models without ATP. The possible bases of the contractile response to CD are discussed. Although direct or indirect action of CD on some microfilaments may occur, a generalized structural disruption of contractile filaments by CD is considered unlikely.

show abstract

“…The effect of CB on isolated mitochondria were demonstrable at about 10 -4 M. Since many of the in vivo effects of the drug such as inhibition of glucose transport [7][8][9] and cytokinesis [3,4] occur rapidly at 10 -7 to 10 -6 M, it seems unlikely that these effects are the result of inhibition of mitochondrial energy production by the drug. In accordance with this conclusion, it was reported [15 ] that ATP concentrations of fibroblasts were unaffected by CB at 2 X 10 -5 M. However, in certain instances, such as the inhibition of cytoplasmic streaming in Nitella [2] and in Elodea (S. Lin, unpublished) and the inhibition of the beating of embryonic heart cells [10], concentrations of CB approaching 10 -4 M are used. These cellular processes have been shown to be dependent on high levels of ATP [16,17] and it is therefore possible that inhibition by CB in these cases can be attributed to the effects of CB on mitochondria of these cells in the manner described in the present report.…”

Section: Discussionmentioning

confidence: 71%