Cytochalasin J treatment significantly alters mitotic spindle microtubule organization and kinetochore structure in PtK1 cells

Wrench, Gregory A.; Snyder, Judith A.

doi:10.1002/(sici)1097-0169(1997)36:2<112::aid-cm2>3.0.co;2-b

Cited by 17 publications

(8 citation statements)

References 36 publications

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“…CJ has previously been shown to block or slow chromosome motion and perturb MT architecture in all stages of mitosis in PtK 1 cells (Snyder and Cohen, 1995;Wrench and Snyder, 1997). Ultrastructural analysis of kinetochores in mitotic PtK 1 cells treated with low concentrations of CJ show a loss of the trilaminar kinetochore structure, associated with the detachment of chromosomes (Wrench and Snyder, 1997), leading us to conclude that CJ sensitive molecules reside in this structure. There is a possibility that actin filaments are not the only target molecule for CJ.…”

Section: Discussionmentioning

confidence: 74%

“…Over-expression of either the p50 subunit of dynactin (Echeverri et al, 1996) or Arp1 (Clark and Meyer, 1992) shows effects on the disruption of the mitotic spindle similar to the CJ treatments described above (Wrench and Snyder, 1997). Clark and Meyer (1992) carried out over-expression studies of normal and mutant Arp1 (centractin) and found that Arp1 may play a crucial role in recruiting components necessary for spindle pole formation and may perturb prometaphase-metaphase transition.…”

Section: Discussionmentioning

confidence: 86%

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Redistribution of motor proteins by Cytochalasin J treatment

Robinson

Snyder

2003

Cell Biology International

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In this study we extend our analysis of the effect of Cytochalasin J (CJ) on mitotic and interphase cells by the use of immunocytochemical techniques to localize antigens to anti-beta-tubulin, anti-dynein heavy chain (HC), anti-dynein intermediate chain (IC), and anti-kinesin antibodies following CJ treatment. Anti-dynein IC and HC staining of CJ treated cells showed a significant reduction in anti-dynein staining in the nuclear region of interphase cells. Monolayer cultures of PtK(1)cells treated with 10 microg/ml CJ for 10 min showed a significant reduction in pixel luminosity of fluorescence staining using anti-dynein IC and HC antibodies (P<0.05). Cytochalasin J treatment reorganized anti-dynein staining from a cytoplasmic punctate staining with greatest intensity in the perinuclear region, to a more uniform staining throughout the cytoplasm.

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Section: Discussionmentioning

confidence: 74%

Section: Discussionmentioning

confidence: 86%

Redistribution of motor proteins by Cytochalasin J treatment

Robinson

Snyder

2003

Cell Biology International

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“…In crane-fly spermatocytes, these effects of cytochalasin D are reversible (Forer and Pickett-Heaps 1998). When cytochalasin J was applied to PtK cells, the cells altered spindle microtubules organization, chromosome congression and the integrity of the mitotic apparatus (Snyder and Cohen 1995;Wrench and Snyder 1997). Thus, these results suggested that actin filaments associate with chromosome segregation and formation of mitotic apparatus.…”

Section: Introductionmentioning

confidence: 87%

Localization of actin filaments on mitotic apparatus in tobacco BY-2 cells

Yasuda

Kanda

Koiwa

et al. 2005

Planta

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Actin filaments are among the major components of the cytoskeleton, and participate in various cellular dynamic processes. However, conflicting results had been obtained on the localization of actin filaments on the mitotic apparatus and their participation in the process of chromosome segregation. We demonstrated by using rhodamine-phalloidin staining, the localization of actin filaments on the mitotic spindles of tobacco BY-2 cells when the cells were treated with cytochalasin D. At prophase, several clear spots were observed at or near the kinetochores of the chromosomes. At anaphase, the actin filaments that appeared to be pulling chromosomes toward the division poles were demonstrated. However, as there was a slight possibility that these results might have been the artifacts of cytochalasin D treatment or the phalloidin staining, we analyzed the localization of actin filaments at the mitotic apparatus immunologically. We cloned a novel BY-2 alpha-type actin cDNA and prepared a BY-2 actin antibody. The fluorescence of the anti-BY-2 actin antibody was clearly observed at the mitotic apparatus in both non-treated and cytochalasin D-treated BY-2 cells during mitosis. The facts that similar results were obtained in both actin staining with rhodamine-phalloidin and immunostaining with actin antibody strongly indicate the participation of actin in the organization of the spindle body or in the process of chromosome segregation. Furthermore, both filamentous actin and spindle bodies disappeared in the cells treated with propyzamide, which depolymerizes microtubules, supporting the notion that actin filaments are associated with microtubules organizing the spindle body.

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“…Under these experimental conditions inhibition of myosin with two different drugs, [180]. Increasing cytochalasins to concentrations exceeding those needed to inhibit cytokinesis in this or other systems, caused alterations in kinetochore structure and lagging chromosomes during anaphase [180,181]. Nevertheless, in a different set of studies, treatment of crane fly spermatocytes with calyculin A, which hyperactivates myosin, was shown to accelerate poleward chromosome movement [182].…”

Section: Actin/myosinmentioning

confidence: 98%

The perpetual movements of anaphase

Maiato

Lince-Faria

2010

Cell. Mol. Life Sci.

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One of the most extraordinary events in the lifetime of a cell is the coordinated separation of sister chromatids during cell division. This is truly the essence of the entire mitotic process and the reason for the most profound morphological changes in cytoskeleton and nuclear organization that a cell may ever experience. It all occurs within a very short time window known as "anaphase", as if the cell had spent the rest of its existence getting ready for this moment in an ultimate act of survival. And there is a good reason for this: no space for mistakes. Problems in the distribution of chromosomes during cell division have been correlated with aneuploidy, a common feature observed in cancers and several birth defects, and the main cause of spontaneous abortion in humans. In this paper we critically review the mechanisms of anaphase chromosome motion that resisted the scrutiny of more than one hundred years of research as part of a tribute to the pioneering work of Miguel Mota.

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Cytochalasin J treatment significantly alters mitotic spindle microtubule organization and kinetochore structure in PtK1 cells

Cited by 17 publications

References 36 publications

Redistribution of motor proteins by Cytochalasin J treatment

Redistribution of motor proteins by Cytochalasin J treatment

Localization of actin filaments on mitotic apparatus in tobacco BY-2 cells

The perpetual movements of anaphase

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