Cytochemical Characterization of Mouse L1210 Leukemia

Kawiak, Jerzy; Skórski, Tomasz; Ciechanowicz, Andrzej; Zylińska, M.; Seidel, H.; Kawalec, M; Gnatowski, B; Czarnomska, A

doi:10.3109/08820138809030587

Cited by 7 publications

(6 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…, ), tumour cell destruction (Kawiak et al. , ) and the induction of cellular growth and differentiation (Khopade et al. , ).…”

Section: Cytosolic/extranuclear Stainingmentioning

confidence: 99%

“…Lectins are carbohydrate-binding proteins that possess various carbohydrate specificities (Leathem & Atkins, 2006). They are widely used in histology and cytology for many purposes such as the identification of carbohydrate moieties of membrane components (Lutsik et al, 1989), tumour cell destruction (Kawiak et al, 1988) and the induction of cellular growth and differentiation (Khopade et al, 1998). The abovementioned three lectins are not cytotoxic for many cell types and all of these detected more binding sites on late apoptotic cells when compared with early apoptotic and vital cells.…”

Section: Lectin Stainingmentioning

confidence: 99%

See 1 more Smart Citation

Cell‐death assessment by fluorescent and nonfluorescent cytosolic and nuclear staining techniques

Atale

Gupta

Yadav³

et al. 2014

Journal of Microscopy

278

171

View full text Add to dashboard Cite

Summary Apoptosis, a genetically programmed cellular event leads to biochemical and morphological changes in cells. Alterations in DNA caused by several factors affect nucleus and ultimately the entire cell leading to compromised function of the organ and organism. DNA, a master regulator of the cellular events, is an important biomolecule with regards to cell growth, cell death, cell migration and cell differentiation. It is therefore imperative to develop the staining techniques that may lead to visualize the changes in nucleus where DNA is housed, to comprehend the cellular pathophysiology. Over the years a number of nuclear staining techniques such as propidium iodide, Hoechst‐33342, 4’, 6‐diamidino‐2‐phenylindole (DAPI), Acridine orange–Ethidium bromide staining, among others have been developed to assess the changes in DNA. Some nonnuclear staining techniques such as Annexin‐V staining, which although does not stain DNA, but helps to identify the events that result from DNA alteration and leads to initiation of apoptotic cell death. In this review, we have briefly discussed some of the most commonly used fluorescent and nonfluorescent staining techniques that identify apoptotic changes in cell, DNA and the nucleus. These techniques help in differentiating several cellular and nuclear phenotypes that result from DNA damage and have been identified as specific to necrosis or early and late apoptosis as well as scores of other nuclear deformities occurring inside the cells.

show abstract

“…, ), tumour cell destruction (Kawiak et al. , ) and the induction of cellular growth and differentiation (Khopade et al. , ).…”

Section: Cytosolic/extranuclear Stainingmentioning

confidence: 99%

Section: Lectin Stainingmentioning

confidence: 99%

Cell‐death assessment by fluorescent and nonfluorescent cytosolic and nuclear staining techniques

Atale

Gupta

Yadav³

et al. 2014

Journal of Microscopy

278

171

View full text Add to dashboard Cite

show abstract

“…Lymphoid leukaemia L1210 [10] syngeneic for DBA/2 mice, received from the National Cancer Institute tumour bank through Arthur D. Little Inc., Acorn Park, Mass., was used. The leukaemia cells proliferate intensively (five L1210 cells/mouse injected i.p.…”

Section: Methodsmentioning

confidence: 99%

Successful adoptive immunotherapy of minimal residual disease after chemoradiotherapy and transplantation of bone marrow purged of leukaemia with mafosfamide

Skórski

Kawalec

Kawiak

1990

Cancer Immunol Immunother

View full text Add to dashboard Cite

The effectiveness of adoptive immunotherapy in eliminating minimal residual disease in tumour-bearing mice after bone marrow transplantation was tested. This model mimics the human clinical condition when autologous bone marrow was purged ex vivo of leukaemia with mafosfamide or was not purged, and stored in liquid nitrogen before transplantation. Animals with minimal residual disease were prepared with marrow-ablative but leukaemia-noncurative doses of cyclophosphamide (CY) and total body irradiation followed by bone marrow transplantation. The next day after transplantation the recipients were injected with splenocytes immunized against the leukaemia cells (Imm-SPL) or monoclonal antibody (mAb). All the control mice died from leukaemia relapse, but 51% of purged bone marrow recipients, which received Imm-SPL, were cured. In similar conditions mAb did not exert a therapeutic effect. Imm-SPL were not able to eradicate minimal residual disease in the recipients of nonpurged bone marrow. Thus, in an animal model, we demonstrated that purging of bone marrow before grafting seems to be indispensable for successful adoptive immunotherapy of minimal residual disease (MRD) after autologous bone marrow transplantation.

show abstract

“…Lectins are carbohydrate‐binding proteins that possess different carbohydrate specificities (12). They are widely used in histology and cytology for different purposes, such as the identification of carbohydrate moieties of membrane components (13), tumor cell destruction (14), and the induction of cellular growth and differentiation (15). Here we propose using specific plant lectins to follow the expression of plasma membrane glycoproteins at apoptosis.…”

mentioning

confidence: 99%

Lectinocytochemical detection of apoptotic murine leukemia L1210 cells

Bilyy

Stoika

2003

Cytometry Pt A

View full text Add to dashboard Cite

Background Various cytomorphologic and biochemical markers of apoptosis are found in different compartments (plasma membrane, cytoplasm, nucleus, and mitochondria) of target cells. Although the plasma membrane is an easily accessible cellular compartment, relatively little is known about its changes during apoptosis. We investigated whether specific changes in the expression of plasma membrane glycoproteins take place during apoptosis and whether these changes could be used for a quantitative estimation of apoptosis. Methods Lectin cytochemical study of normal and apoptotic murine leukemia cells of the L1210 line was done. Horseradish peroxidase–labeled Laburnum anagyroides bark agglutinin, Phaseolus vulgaris agglutinin, Pisum sativum lectin (PSL), Ricinus communis agglutinin (RCA‐120; 120 kDa), Solanum tuberosum agglutinin, Triticum vulgaris lectin (wheat germ agglutinin), Viscum album agglutinin, Canavalia ensiformis lectin (concanavalin A), and Helix pomatia lectin were used to compare specific glycoprotein expressions in normal and apoptotic murine leukemia cells of the L1210 line sensitive (L1210) and resistant (L1210R) to apoptosis induction by cisplatin. Results The data demonstrated significantly increased binding of α‐D‐mannose–specific PSL lectin (P < 0.01) and β‐D‐galactose–specific RCA‐120 lectin (P < 0.001) by the apoptotic cells of the L1210 and L1210R lines in comparison with the intact cells. That binding was shown to be specific because it was blocked by the corresponding inhibitory sugars. Conclusions Lectins specific to α‐D‐mannose (PSL) and β‐D‐galactose (RCA‐120) can be used to distinguish between native and apoptotic murine leukemia L1210 cells and to quantitatively estimate apoptosis in a population of these cells. Cytometry Part A 56A:89–95, 2003. © 2003 Wiley‐Liss, Inc.

show abstract

Cytochemical Characterization of Mouse L1210 Leukemia

Cited by 7 publications

References 13 publications

Cell‐death assessment by fluorescent and nonfluorescent cytosolic and nuclear staining techniques

Cell‐death assessment by fluorescent and nonfluorescent cytosolic and nuclear staining techniques

Successful adoptive immunotherapy of minimal residual disease after chemoradiotherapy and transplantation of bone marrow purged of leukaemia with mafosfamide

Lectinocytochemical detection of apoptotic murine leukemia L1210 cells

Contact Info

Product

Resources

About