Cytochemical demonstration of ferric iron and fluorescence microscopy observations on ‘Gomori positive’ glia grownin vitro

Catechol oxidation by peroxidase-positive astrocytes in primary culture: an electron spin resonance study

“…2). The autofluorescence bleaches rapidly when cultures are examined live but is preserved by formalin fixation analogous to the observations of Srebro and Macinska (1972) in diencephalic explants. We have previously demonstrated that the induced peroxidase activity is nonenzymatic in nature (Schipper et al, 1990b).…”

Histochemical study on endogenous diaminobenzidine-positive granules in the glia cell of rat brain.

“…The granules of Gomori-positive glia cells have suggested the presence of endogenous peroxidase activity. Moreover, the granules reacting with Gomori's stain and the granules exhibiting endogenous peroxidase activity are identical (18,19,21). Gomori-positive granules or endogenous peroxidase positive granules in the filial cells were demonstrated with the thiol group (18), ferric iron (19) and calcium and sulphur (21) by means of histochemical and microprobe analytical studies.…”

“…The so-called `Gomori-positive' glia cells are characterized by the presence of abundant cytoplasmic granulations strongly staining with Gomori's chrome hematoxylin following oxidation with acid permanganate (5,13,21,17,18,19) . The granules of Gomori-positive glia cells have suggested the presence of endogenous peroxidase activity.…”

Differential effects of cysteamine on heat shock protein induction and cytoplasmic granulation in astrocytes and glioma cells

“…Indeed, the presence of iron in these inclusions has been demonstrated using Perl's ferric ferrocyanide method (Srebro et al, 1972) and by Xray microprobe ana1ysis (McLaren et al, 1992;Brawer et al, 1994a)..…”

“…Gomori-Positive Astrocytes in Primary Culture Srebro and Mackinska (1972) first detected the presence of Gomori-positive brain ceUs in fetal rat and human explants. They postulated that these ceUs were glial in origin because their tinctorial and fluorescent properties were similar to those of periventricular astrocytes in sitll.…”