Cytochemical localization of adenylate cyclase and guanylate cyclase in the parietal cell of guinea pig gastric gland.

Cytochemical localization of Ca++-ATPase, H+,K+-ATPase and Na+,K+-ATPase in acid-secreting parietal cell and non-secreting parietal cell.

“…We have also found that adenylate cyclase activity and guanylate cyclase activity were localized on the lateral apical membrane of the parietal cell in the guinea pig gastric gland (36) and on the gap junction in various kinds of cells (14,35). The morphological study using freeze-fracture method indicated that gap junction was not recognized in the rat and mouse parietal cells (22).…”

Lead citrate method of guanylate cyclase and its application to tissue receiving rapid freeze substitution fixation.

“…In 1975, the first attempt on GCase demonstration was reported (53,54). GCase activity was reported in the rat retina and some other tissues (19,20,29,40,63,69). However, LeMay and Jarett (1975) claimed that in the use of the lead ion as capture agent in ACase histochemistry, the lead ion not only brings about a complete inhibition of the enzyme activity, at the concentration in the reaction medium, but it further acts to catalize the non enzymatic degradation of the substrate (31).…”

Ca2+-ATPase activity in the gastric parietal cells of rat during perinatal development.

“…However, the studies of Cat+-ATPase in the gastric parietal cells are very few (36) and more so during development.…”