Cytochrome c Gene and Protein Expression: Developmental Regulation, Environmental Response, and Pesticide Sensitivity in Aedes aegypti

Zhao, Liming; Pridgeon, Julia W.; Becnel, James J.; Clark, Gary G.; Linthicum, Kenneth J.

doi:10.1093/jmedent/45.3.401

Cited by 9 publications

(11 citation statements)

References 24 publications

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“…The PCR primers for actin gene were CxActin629-F 5Ј-TGCGTGACATCAAGGAGAAG-3Ј and CxActin-893R 5Ј-GTGTTGGCGTACAGGTC-CTT-3Ј. The PCR thermal cycling parameters were the same as previously described (Zhao et al 2008(Zhao et al , 2009. Relative expression levels were calculated as follows.…”

Section: Methodsmentioning

confidence: 99%

Identification and Expression Profile of Multiple Genes in Response to Magnesium Exposure in Culex quinquefasciatus Larvae

Zhao¹,

Becnel²,

Clark³

et al. 2010

jnl. med. entom.

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Magnesium is crucial for baculovirus transmission in Culex nigripalpus (Theobald) and Culex quinquefasciatus (Say) larvae. However, the mechanistic role of magnesium in baculovirus transmission is unknown. To investigate the possible role of host response factors in baculovirus transmission, suppression subtractive hybridization was used to identify genes differentially transcribed after magnesium exposure in Cx. quinquefasciatus larvae. Suppression subtractive hybridization was performed in both directions enriching for cDNAs differentially transcribed between a nonmagnesium larval control and magnesium (15 mM MgSO4) treatment of Cx. quinquefasciatus larvae held for 1 h at 27 degrees C. Clones from differentially transcribed genes were evaluated by sequencing, and relative gene transcription levels were analyzed using quantitative real-time polymerase chain reaction quantitative real-time polymerase chain reaction. Target transcripts up/downregulated by magnesium included Cx. quinquefasciatus troponin C, isocitrate dehydrogenase, allergen, cytochrome b5, chymotrypsinogen, apolipophorins, tryptase gamma, carboxylesterase, prolylcarboxypeptidase, imaginal disc growth factor, aldehyde dehydrogenase, tropomyosin-1, chitotriosidase, heat shock protein 70 B2, inorganic phosphate cotransporter, and many other hypothetical protein genes. Magnesium can alter gene transcription in a vector mosquito population, and understanding this process can provide insight into the mechanistic role of magnesium in baculovirus transmission.

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Section: Methodsmentioning

confidence: 99%

Identification and Expression Profile of Multiple Genes in Response to Magnesium Exposure in Culex quinquefasciatus Larvae

Zhao¹,

Becnel²,

Clark³

et al. 2010

jnl. med. entom.

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“…The PCR thermal cycling parameters were the same as described previously. 22,24 Relative expression levels were calculated as follows. First, SiGNBP transcript levels relative to a standard (SiActin) were calculated using the following formula:…”

Section: Qpcr Amplificationmentioning

confidence: 99%

“…8,9 As in all organisms, the development of ants requires multigene regulation, which is affected by environmental conditions such as temperature, radiation, and challenges from pesticides and parasites. [10][11][12][13][14][15][16][17][18][19][20][21][22] However, the role of GNBPs in Solenopsis invicta Buren development has never been examined. In this study, the complete cDNA of the guanine nucleotide-binding protein gene β-subunit (SiGNBP) was cloned and sequenced from S. invicta workers, and its transcriptional expression related to the developmental stages and pesticide response was examined.…”

Section: Introductionmentioning

confidence: 99%

Transcription profiling of guanine nucleotide binding proteins during developmental regulation and pesticide response in Solenopsis invicta (Hymenoptera: Formicidae)

Zhao¹,

Chen²,

Jones³

2012

OAIP

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Guanine nucleotide binding proteins (GNBP), glycoproteins anchored on the cytoplasmic cell membrane, are mediators for many cellular processes. Complete cDNA of the GNBP gene β-subunit (SiGNBP) was cloned and sequenced from Solenopsis invicta Buren (Hymenoptera: Formicidae) worker ants. To understand whether SiGNBP is developmentally regulated in S. invicta, its expression levels in different developmental stages were examined using quantitative real-time polymerase chain reaction. SiGNBP was expressed in each developmental stage, and was especially highly expressed in the late larval and early pupal stages, as well among the dealate females (queens) ∼10 days post nuptial flight. Quantitative real-time polymerase chain reaction also showed that mRNA transcription levels of SiGNBP in S. invicta workers were regulated during the duration of the study in response to heat shock, ultraviolet light, and boric acid treatment. These results suggest that the SiGNBP gene may play an important role in the development of S. invicta, and this has potential for use as a target in new insecticides for the control of fire ants.

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“…The PCR thermal cycling parameters were also the same as previously published. 24,27 Relative expression levels were determined as follows: first, AetNADH transcript levels relative to a standard (ribosomal 40s/60s) were obtained by applying the formula ∆CT = CT (AetNADH) -CT (Aet-ribosomal 40s/60s). Second, an average ∆CT value for each sample was determined.…”

mentioning

confidence: 99%

“…Third, relative expression levels were evaluated applying the modified equation 100×2 -[average ∆CT] . 28,29 Sequence data analysis A multiple sequence alignment of AetNDUFS8 and AetNADH5 as well as orthologs from other mosquitoes were performed with the MEGA 5.05 program (http://www.megasoftware.net). The Neighbor-joining method with the MEGA 5.05 program was used to construct the phylogenetic trees.…”

mentioning

confidence: 99%

Identification and transcription profiling of NDUFS8 in Aedes taeniorhynchus (Diptera: Culicidae): developmental regulation and environmental response

Zhao

Kline²,

Becnel³

et al. 2014

OAIP

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The cDNA of a NADH dehydrogenase-ubiquinone Fe-S protein 8 subunit (NDUFS8) gene from Aedes (Ochlerotatus) taeniorhynchus Wiedemann has been cloned and sequenced. The 824 bp full-length mRNA sequence of AetNDUFS8 encodes an open reading region of 651 bp (that is, 217 amino acids). To identify if AetNDUFS8 is developmentally regulated, we employed a quantitative real-time polymerase chain reaction to examine AetNDUFS8 gene expression levels in all developmental stages of Ae. taeniorhynchus. In egg, larval, and pupal stages, AetNDUFS8 was expressed at relatively low levels. However, in the teneral adult stage, AetNDUFS8 was highly expressed. During the time course study in response to permethrin pesticide treatment, quantitative real-time polymerase chain reaction (PCR) also showed that mRNA transcription levels of AetNDUFS8 were regulated in female Ae. taeniorhynchus. A mitochondrially encoded NADH dehydrogenase subunit 5 AetNADH5 was highly expressed in different developmental stages of Ae. taeniorhynchus. This study suggests that AetNDUFS8 and AetNADH5 play an essential role in the development of Ae. taeniorhynchus and will provide information useful for developing dsRNA pesticide for mosquito control.

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Cytochrome c Gene and Protein Expression: Developmental Regulation, Environmental Response, and Pesticide Sensitivity in Aedes aegypti

Cited by 9 publications

References 24 publications

Identification and Expression Profile of Multiple Genes in Response to Magnesium Exposure in Culex quinquefasciatus Larvae

Identification and Expression Profile of Multiple Genes in Response to Magnesium Exposure in Culex quinquefasciatus Larvae

Transcription profiling of guanine nucleotide binding proteins during developmental regulation and pesticide response in Solenopsis invicta (Hymenoptera: Formicidae)

Identification and transcription profiling of NDUFS8 in Aedes taeniorhynchus (Diptera: Culicidae): developmental regulation and environmental response

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