Cytochrome P450 2F2 (CYP2F2) negatively regulates browning in 3T3-L1 white adipocytes

Dang, Trang Thi Huyen; Choi, Minsu; Pham, Huong Giang; Yun, Jong Won

doi:10.1016/j.ejphar.2021.174318

Cited by 4 publications

(3 citation statements)

References 34 publications

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“…Bpifa1 is a critical component of the innate immune response that prevents upper airway diseases [ 42 ]. Cytochromes P450 (CYPs) are a multigene superfamily of constitutively expressed and inducible enzymes responsible for the detoxification of many endogenous and exogenous compounds and for the metabolism of numerous medications [ 43 ]. LTF is a protective gene in PRAD, and its downregulation will inhibit the normal immune response in the body [ 44 ].…”

Section: Resultsmentioning

confidence: 99%

Cellular and molecular mechanisms of fibrosis and resolution in bleomycin-induced pulmonary fibrosis mouse model revealed by spatial transcriptome analysis

Li,

Wang,

et al. 2023

Heliyon

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Section: Resultsmentioning

confidence: 99%

Cellular and molecular mechanisms of fibrosis and resolution in bleomycin-induced pulmonary fibrosis mouse model revealed by spatial transcriptome analysis

Li,

Wang,

et al. 2023

Heliyon

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“…4 Apart from classical regulators, such as PRDM16, PGC1α, IRF4, Zfp516, FoxC2, and EHMT1, numerous transcriptional modulators responsible for fat browning have recently been identified. 4,13 More recently, numerous negative regulators of fat browning have been identified, including CYP2E1 41 , CYP2F2 40 , FAM107A, 9 LCP1, 10 and ADAMTS15. 11 The UCP1 protein plays a significant role in regulating the thermogenic program in brown and beige adipocytes, 8 and the upregulation of UCP1 by CYFIP2 deficiency was F I G U R E 6 CYFIP2 deficiency stimulates the browning of 3T3-L1 white adipocytes by inhibiting the GABA-BR pathway.…”

Section: Discussionmentioning

confidence: 99%

“…Apart from classical regulators, such as PRDM16, PGC1α, IRF4, Zfp516, FoxC2, and EHMT1, numerous transcriptional modulators responsible for fat browning have recently been identified 4,13 . More recently, numerous negative regulators of fat browning have been identified, including CYP2E1 41 , CYP2F2 40 , FAM107A, 9 LCP1, 10 and ADAMTS15 11 …”

Section: Discussionmentioning

confidence: 99%

Loss of cytoplasmic FMR1‐interacting protein 2 (CYFIP2) induces browning in 3T3‐L1 adipocytes via repression of GABA‐BR and activation of mTORC1

Manigandan

Yun

2022

J of Cellular Biochemistry

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Obesity and related metabolic disorders are epidemic diseases. Promoting thermogenesis and a functional increase in the browning of white adipocytes may counteract obesity. On the other hand, the molecular mechanism that regulates brown and beige fat-mediated thermogenesis is unclear. This article reports a molecular network led by cytoplasmic FMR1-interacting protein 2 (CYFIP2) that negatively regulates adipocyte browning in white adipocytes. Although the function of CYFIP2 in Fragile X Syndrome (FXS) and autism have been reported, its physiological roles in adipocytes remain elusive. Therefore, this study examined the physiological consequences of its deprivation in cultured 3T3-L1 white adipocytes using loss-of-function studies. Combined real-time quantitative reverse-transcription polymerase chain reaction and immunoblot analysis showed that the loss of CYFIP2 induces fat browning, as evidenced by the gene and protein expression levels of the brown fat-associated markers. A deficiency of CYFIP2 promoted mitochondrial biogenesis and significantly enhanced the expression of the core set beige fat-specific genes (Cd137, Cidea, Cited1, Tbx1, and Tmem26) and proteins (PGC-1α, PRDM16, and UCP1). In addition, a CYFIP2 deficiency promoted lipid catabolism and suppressed adipogenesis, lipogenesis, and autophagy. A mechanistic study showed that the loss of CYFIP2 induces browning in white adipocytes, independently via the activation of mTORC1 and suppression of the GABA-BR signaling pathway. The present data revealed a previously unidentified mechanism of CYFIP2 in the browning of white adipocytes and emphasized the potential of CYFIP2 as a pharmacotherapeutic target for treating obesity and other metabolic disorders.

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Dopamine receptor D4 (DRD4) negatively regulates UCP1- and ATP-dependent thermogenesis in 3T3-L1 adipocytes and C2C12 muscle cells

Haddish

Yun

2023

Pflugers Arch - Eur J Physiol

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Cytochrome P450 2F2 (CYP2F2) negatively regulates browning in 3T3-L1 white adipocytes

Cited by 4 publications

References 34 publications

Cellular and molecular mechanisms of fibrosis and resolution in bleomycin-induced pulmonary fibrosis mouse model revealed by spatial transcriptome analysis

Cellular and molecular mechanisms of fibrosis and resolution in bleomycin-induced pulmonary fibrosis mouse model revealed by spatial transcriptome analysis

Loss of cytoplasmic FMR1‐interacting protein 2 (CYFIP2) induces browning in 3T3‐L1 adipocytes via repression of GABA‐BR and activation of mTORC1

Dopamine receptor D4 (DRD4) negatively regulates UCP1- and ATP-dependent thermogenesis in 3T3-L1 adipocytes and C2C12 muscle cells

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