Cytochrome P450 Biodiversity and Plant Protection

Ohkawa, Hideo; Tsujii, Hisae; Shimoji, Miyuki; Imajuku, Yoshiro; Imaishi, Hiromasa

doi:10.1584/jpestics.24.197

Cited by 14 publications

(5 citation statements)

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“…Xenobiotics, such as pesticides, in plants are catalyzed by xenobiotic-metabolizing enzymes, such as cytochrome P450. Ohkawa et al [28] reviewed the works for P450 and they suggested that over 360 of P450 had been found in plants by 1999, and their function was still in the process of being identified. For example, CYP76B1 from Helianthus tuberosus has the characteristic of oxidizing several phenylurea herbicides.…”

Section: Differences In Dieldrin-uptake Property Between Plantsmentioning

confidence: 99%

Dieldrin uptake and translocation in plants growing in hydroponic medium

Murano

Otani

Seike

et al. 2009

Enviro Toxic and Chemistry

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It has been known that the Cucurbitaceae family takes up a large amount of persistent organic pollutants from soils and that the translocation of those compounds in cucurbits is higher than those in non-cucurbits. To understand the persistent organic pollutant uptake mechanisms of plant species, we compared the dieldrin absorption and transportation potentials of several plants in hydroponic medium. Sorghum (Sorghum vulgare Moench), sunflower (Helianthus annuus L.), soybean (Glycine max), komatsuna (Brassica rapa var. peruviridis), white-flowered gourd (Lagenaria siceraria var. hispida), cucumber (Cucumis sativus L.), and zucchini (Cucurbita pepo L.) were grown in a dieldrin-added hydroponic medium for 10 d, and then the amount of dieldrin in their shoots and roots was measured. All of the roots contained dieldrin, whereas only the cucurbits (white-flowered gourd, cucumber, and zucchini) contained considerable amounts of dieldrin in their shoots. The dieldrin uptake to the roots depended on the concentration of the n-hexane soluble components in the roots, regardless of whether the dieldrin in the roots was translocated to shoots or not. The dieldrin uptake from the solution to the roots was thought to be due to a passive response, such as adsorption on the roots. The translocation of dieldrin from the roots to the shoots was probably through the xylems. The amounts of dieldrin in the shoots per transpiration rates were higher for cucurbits than for non-cucurbits. It seems likely that cucurbits have uptake mechanisms for hydrophobic organic chemicals.

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Section: Differences In Dieldrin-uptake Property Between Plantsmentioning

confidence: 99%

Dieldrin uptake and translocation in plants growing in hydroponic medium

Murano

Otani

Seike

et al. 2009

Enviro Toxic and Chemistry

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“…The patterns of expression of detoxifying enzyme-coding genes at various stages of growth and development and in various tissues can, to a certain extent, reveal the functions of genes ( Chung et al, 2009 ). These distinct expression patterns indicate that the proteins are involved in pesticide resistance and breakdown of secondary plant compounds ( Ohkawa et al, 1999 ). Similarly to the findings of previous research ( Xu et al, 2018 ; Hou et al, 2021 ), our findings revealed that MusiDN2722 is expressed in both larvae and adults.…”

Section: Discussionmentioning

confidence: 99%

RNA interference in cytochrome P450 monooxygenase (CYP) gene results in reduced insecticide resistance in Megalurothrips usitatus Bagnall

Chen

Zhou

et al. 2023

Front. Physiol.

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Genes of the cytochrome P450 (CYP450) superfamily are known to be involved in the evolution of insecticide resistance. In this study, the transcriptomes of two Megalurothrips usitatus Bagnall (Thysanoptera: Thripidae) strains (resistant and susceptible) were screened for detoxification genes. MusiDN2722 encodes a protein composed of 504 amino acid residues with a relative molecular mass of 57.3 kDa. Multiple sequence alignment and phylogenetic analysis showed that MusiDN2722 is a member of the CYP450 family and has characteristics of the conserved CYP6 domain shared by typical CYP450 family members. RT-qPCR (real-time quantitative polymerase chain reaction) analysis showed that MusiDN2722 was upregulated in the acetamiprid-resistant strain compared with the susceptible strain (p < 0.05), and the relative expression level was significantly higher at 48 h after exposure than at 24 h after exposure. The interference efficiency of the injection method was higher than that of the membrane-feeding method. Silencing of MusiDN2722 through RNA interference significantly increased the sensitivity of M. usitatus to acetamiprid. Overall, this study revealed that MusiDN2722 plays a crucial role in the resistance of M. usitatus to acetamiprid. The findings will not only advance our understanding of the role of P450s in insecticide resistance but also provide a potential target for the sustainable control of destructive pests such as thrips.

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“…Conjugates are actively transported to the vacuole and, in some cases, to the apoplast by the action of an ATP-dependent membrane pump [125][126][127]. Dihydroxylated pollutants can also be covalently linked with plant cell-wall polymers and lignin [128,129], probably through the action of cell-wall-or vacuole-associated enzymes (i.e., internal peroxidases and laccases). These enzymes, normally involved in the detoxification of H 2 O 2 , have been also associated with the formation of tyrosine or ferulate cross-links between different plant cell wall polymers with the non-specific oxidative polymerization of phenolic units to produce lignin and with the deposition of aromatic residues of suberin on the cell wall [130].…”

Section: Detoxification Of Organic Compoundsmentioning

confidence: 99%

Biochemical and Metabolic Plant Responses toward Polycyclic Aromatic Hydrocarbons and Heavy Metals Present in Atmospheric Pollution

Molina

Segura

2021

Plants

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Heavy metals (HMs) and polycyclic aromatic hydrocarbons (PAHs) are toxic components of atmospheric particles. These pollutants induce a wide variety of responses in plants, leading to tolerance or toxicity. Their effects on plants depend on many different environmental conditions, not only the type and concentration of contaminant, temperature or soil pH, but also on the physiological or genetic status of the plant. The main detoxification process in plants is the accumulation of the contaminant in vacuoles or cell walls. PAHs are normally transformed by enzymatic plant machinery prior to conjugation and immobilization; heavy metals are frequently chelated by some molecules, with glutathione, phytochelatins and metallothioneins being the main players in heavy metal detoxification. Besides these detoxification mechanisms, the presence of contaminants leads to the production of the reactive oxygen species (ROS) and the dynamic of ROS production and detoxification renders different outcomes in different scenarios, from cellular death to the induction of stress resistances. ROS responses have been extensively studied; the complexity of the ROS response and the subsequent cascade of effects on phytohormones and metabolic changes, which depend on local concentrations in different organelles and on the lifetime of each ROS species, allow the plant to modulate its responses to different environmental clues. Basic knowledge of plant responses toward pollutants is key to improving phytoremediation technologies.

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Cytochrome P450 Biodiversity and Plant Protection

Cited by 14 publications

References 1 publication

Dieldrin uptake and translocation in plants growing in hydroponic medium

Dieldrin uptake and translocation in plants growing in hydroponic medium

RNA interference in cytochrome P450 monooxygenase (CYP) gene results in reduced insecticide resistance in Megalurothrips usitatus Bagnall

Biochemical and Metabolic Plant Responses toward Polycyclic Aromatic Hydrocarbons and Heavy Metals Present in Atmospheric Pollution

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