Cytocompatibility of Biodentine and <scp>iR</scp>oot <scp>FS</scp> with human periodontal ligament cells: an <i>in vitro</i> study

Luo, Tao; Liu, Jun; Sun, Yan; Shen, Ya; Zou, Ling

doi:10.1111/iej.12889

Cited by 15 publications

(20 citation statements)

References 39 publications

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“…Moreover, hSCAP cultured in iRoot FS conditioned medium had significantly greater expression levels of ALP and DSPP, and more formation of mineralized nodules than the MTA group. Those data demonstrated that iRoot FS was superior in facilitating osteo/odontogenic differentiation of hSCAP, consistent with previous studies (Peng et al , Sun et al , Luo et al ). However, the detailed molecular mechanism of how iRoot FS promotes osteo/odontogenic differentiation is not reported.…”

Section: Discussionsupporting

confidence: 91%

“…Both iRoot FS and MTA are calcium phosphate silicate cement (CPSC) (Lu & Zhou ). Several studies have indicated that CPSC materials have the potential to boost osteo/odontogenic differentiation by increasing the expression of osteo/odontogenic markers and calcium deposit (Peng et al , Sun et al , Luo et al ). In the present study, the osteo/odontogenic differentiation of hSCAP exposed to both iRoot FS and MTA was enhanced markedly compared to the control group.…”

Section: Discussionmentioning

confidence: 99%

“…Sun et al () examined the effects of iRoot FS on the odontogenic differentiation of human dental pulp stem cells, which suggested that iRoot FS was a promising bioactive material for direct pulp capping. Luo et al () investigated the cytotoxicity and biocompatibility of iRoot FS with human periodontal ligament cells and found that iRoot FS could be used as a root‐end filling material in endodontic surgery. However, it is still unknown whether iRoot FS can induce apical closure of immature permanent teeth, and whether it is suitable as an apical barrier during apexification.…”

Section: Introductionmentioning

confidence: 99%

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Cell migration and osteo/odontogenesis stimulation of iRoot FS as a potential apical barrier material in apexification

Liu

et al. 2019

Int Endodontic J

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Aim To investigate the in vitro biological effects of a nanoparticle bioceramic material, iRoot Fast Set root repair material (iRoot FS), on the proliferation, migration and osteo/odontogenic differentiation of human stem cells from the apical papilla (hSCAP), and to further explore the mechanism involved in osteo/odontogenic induction of iRoot FS. Methodology hSCAP were isolated and characterized in vitro. iRoot FS conditioned medium were prepared and used to treat hSCAP, while using mineral trioxide aggregate (MTA) conditioned medium as the positive control and regular medium as the negative control. MTT assay and BrdU labelling assay were performed to determine cell proliferation. Wound healing assay and transwell assay were conducted to evaluate cell migration. The osteo/odontogenic differentiation of hSCAP was evaluated by qPCR, Western blot and Alizarin red S staining. Wnt inhibitor was used for downregulating the expression level of β‐catenin of hSCAP. Results The cell proliferation of hSACP in the iRoot FS group was not significantly different compared with the control groups. The cell migration of hSCAP in the iRoot FS group was significantly increased than the MTA and negative control groups (P < 0.01). The expression levels of osteo/odontogenic markers and mineralization nodule formation of hSCAP in the iRoot FS group were significantly elevated (P < 0.01). Furthermore, iRoot FS enhanced the osteo/odontogenic differentiation of hSCAP by activating Wnt/β‐catenin signalling. Conclusions iRoot FS promoted the cell migration of hSCAP and enhanced their oseto/odontogenesis potential via the Wnt/β‐catenin pathway without cytotoxicity. iRoot FS had satisfactory biological properties and has potential to be used as an apical barrier in apexification or as a coronal sealing material in regenerative endodontic treatment.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cell migration and osteo/odontogenesis stimulation of iRoot FS as a potential apical barrier material in apexification

Liu

et al. 2019

Int Endodontic J

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“…Additionally, both the cellular processes (migration and adhesion) play a key role in the maintenance of tissue homeostasis, repair and regeneration. Therefore, to make these goals achievable, cells must be able to migrate and adhere to the targeted sites for regeneration and development of functional tissues [33,39]. As previously described [40], dental stem cells respond to these injuries by these cellular processes; proliferation, differentiation, migration and adhesion in order to repair and replace the damaged tissues.…”

Section: Discussionmentioning

confidence: 99%

“…Biodentine™ (BD) is relatively a new calcium silicate-based cement, synthesized to be used in direct contact with the periodontal ligament, which is crucial in conservative therapy. It has been suggested that the attachment of cells to the root surface is a critical and complicated dynamic process, which has implications in biological processes, such as cell proliferation and differentiation [33]. The effect of Biodentine™ on the adhesive ability of fibroblasts of PDL has been reported (40).…”

Section: Discussionmentioning

confidence: 99%

Biocompatibility of Biodentine™ ® with Periodontal Ligament Stem Cells: In Vitro Study

et al. 2020

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Biodentine™ is a tricalcium silicate-based cement material that has a great impact on different biological processes of dental stem cells, compared to other biomaterials. Therefore, we aimed to investigate the optimum biocompatible concentration of Biodentine™ with stem cells derived from periodontal ligament (hPDLSCs) by determining cell proliferation, cytotoxicity, migration, adhesion and mineralization potential. hPDLSCs were treated with Biodentine™ extract at different concentrations; 20, 2, 0.2 and 0.02 mg/mL. Cells cultured without Biodentine™ were used as a blank control. The proliferation potential of hPDLSCs was evaluated by MTT viability analysis for 6 days. Cytotoxicity assay was performed after 3 days by using AnnexinV/7AAD. Migration potential was investigated by wound healing and transwell migration assays at both cellular and molecular levels. The expression levels of chemokines CXCR4, MCP-1 and adhesion molecules FGF-2, FN, VCAM and ICAM-1 were measured by qPCR. The communication potentials of these cells were determined by adhesion assay. In addition, mineralization potential was evaluated by measuring the expression levels of osteogenic markers; ALP, OCN, OPN and Collagen type1 by qPCR. Our results showed significant increase in the proliferation of hPDLSCs at low concentrations of Biodentine™ (2, 0.2 and 0.02 mg/mL) while higher concentration (20 mg/mL) exhibited cytotoxic effect on the cells. Moreover, 2 mg/mL Biodentine™ showed a significant increase in the migration, adhesion and mineralization potentials of the derived cells among all concentrations and when compared to the blank control. Our findings suggest that 2 mg/mL of Biodentine™ is the most biocompatible concentration with hPDLSCs, showing a high stimulatory effect on the biological processes.

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Real‐time evaluation of the biocompatibility of calcium silicate‐based endodontic cements: An in vitro study

Rebolledo

Alcántara-Dufeu

Machuca

et al. 2023

Clinical & Exp Dental Res

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Introduction An ideal filling material should hermetically seal the communication pathways between the canal system and surrounding tissues. Therefore, during the last few years, the development of obturation materials and techniques to create optimal conditions for the proper healing of apical tissues has been a focus of interest. The effects of calcium silicate‐based cements (CSCs) on periodontal ligament cells have been investigated, and promising results have been obtained. To date, there are no reports in the literature that have evaluated the biocompatibility of CSCs using a real‐time live cell system. Therefore, this study aimed to evaluate the real‐time biocompatibility of CSCs with human periodontal ligament cells (hPDLCs). Methodology hPDLC were cultured with testing media of endodontic cements for 5 days: TotalFill‐BC Sealer, BioRoot RCS, Tubli‐Seal, AH Plus, MTA ProRoot, Biodentine, and TotalFill‐BC RRM Fast Set Putty. Cell proliferation, viability, and morphology were quantified using real‐time live cell microscopy with the IncuCyte S3 system. Data were analyzed using the one‐way repeated measures (RM) analysis of variance multiple comparison test ( p < .05). Results Compared to the control group, cell proliferation in the presence of all cements was significantly affected at 24 h ( p < .05). ProRoot MTA and Biodentine lead to an increase in cell proliferation; there were no significant differences with the control group at 120 h. In contrast, Tubli‐Seal and TotalFill‐BC Sealer inhibited cell growth in real‐time and significantly increased cell death compared to all groups. hPDLC co‐cultured with sealer and repair cements showed a spindle‐shaped morphology except with cements Tubli‐Seal and TotalFill‐BC Sealer where smaller and rounder cells were obtained. Conclusions The biocompatibility of the endodontic repair cements performed better than the sealer cements, highlighting the cell proliferation of the ProRoot MTA and Biodentine in real‐time. However, the calcium silicate‐based TotalFill‐BC Sealer presented a high percentage of cell death throughout the experiment similar to that obtained.

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Cytocompatibility of Biodentine and iRoot FS with human periodontal ligament cells: an in vitro study

Abstract: iRoot FS had a positive effect on the adhesion, proliferation and biomineralization of hPDLCs compared with Biodentine.

Cited by 15 publications

References 39 publications

Cell migration and osteo/odontogenesis stimulation of iRoot FS as a potential apical barrier material in apexification

Cell migration and osteo/odontogenesis stimulation of iRoot FS as a potential apical barrier material in apexification

Biocompatibility of Biodentine™ ® with Periodontal Ligament Stem Cells: In Vitro Study

Real‐time evaluation of the biocompatibility of calcium silicate‐based endodontic cements: An in vitro study

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