Cytocompatibility of HeLa Cells to Nano-Sized Ceramics Particles

Abstract

In this study, we investigated the behaviors and cytocompatibility response of human cervical carcinoma (HeLa) cells expose to nano-sized particles. Cultivated cells exposed to titanium oxide and indium oxide nanoparticles remained highly viable. In the presence of copper oxide (CuO); however, the cells became seriously inflamed. To understand the mechanism by which CuO causes cell death, we evaluated cell death and apoptosis cytometry. CuO induced cells apoptosis more strongly than exposure to titania nanoparticles. Confocal fluorescence microscopy revealed that the nano-sized particles penetrate the cells.

Cytocompatiblity of Ceramic Nanoparticles to Various Types of Cells

“…Therefore, the CuO particles affected the cells because of their interaction with the surface caused by the contact. In a previous study, it was reported that CuO exposure induced apoptotic cell death in Hella cells [11]. Karlsson reported that CuO nanoparticles showed cytotoxicity by causing oxidative lesions and increasing intracellular reactive oxygen species [5].…”

Quaternized Sodium Alginate-g-Ethyl-Oxazoline Copolymer Brushes and Their Supramolecular Networks via Hydrogen Bonding

“…Some studies have reported that cancer cells such as HeLa cells and human lung cancer cells (A549) were used to evaluate cell compatibility. When the materials show low cytotoxicity to cancer cells, they also have cell compatibility with normal cells. − The cytotoxicity of the quaternized alginate-based copolymer was tested against normal cells and HeLa cells in our previous work, which shows that quaternized alginate-based copolymers have good biocompatibility for both cells. The CCK-8 method was used to explore the cytotoxicity of the QSA 769 - g 485 -(EOX) n copolymer brush against HeLa cells, and the experimental results under concentration from 20 to 500 μg/mL are shown in Figure .…”