Cytocompatibility of polymer-based periodontal bone substitutes in gingival fibroblast and MC3T3 osteoblast cell cultures

Ruediger, Tina; Berg, Albrecht; Guellmar, André; Rode, Claudia; Schnabelrauch, Matthias; Urbanek, Annett; Wagner, Kerstin; Wyrwa, Ralf; Kinne, Raimund W.; Sigusch, Bernd W.

doi:10.1016/j.dental.2012.05.008

Cited by 18 publications

(12 citation statements)

References 62 publications

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“… 35 In our studies, we focus on poly(vinyl butyral-co-vinyl alcohol-co-vinyl acetate) (PVB), which has not been previously applied as a porous membrane for use as a skin patch; however, the PVB thin film was assessed as a prospective dental material. 36 …”

Section: Introductionmentioning

confidence: 99%

Nano- and Microfiber PVB Patches as Natural Oil Carriers for Atopic Skin Treatment

Krysiak

Kaniuk

Metwally

et al. 2020

ACS Appl. Bio Mater.

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Atopic dermatitis (eczema) is a widespread disorder, with researchers constantly looking for more efficacious treatments. Natural oils are reported to be an effective therapy for dry skin, and medical textiles can be used as an alternative or supporting therapy. In this study, fibrous membranes from poly(vinyl butyral-co-vinyl alcohol-co-vinyl acetate) (PVB) with low and high molecular weights were manufactured to obtain nano- and micrometer fibers via electrospinning for the designed patches used as oil carriers for atopic skin treatment. The biocompatibility of PVB patches was analyzed using proliferation tests and scanning electron microscopy (SEM), which combined with a focused ion beam (FIB) allowed for the 3D visualization of patches. The oil spreading tests with evening primrose, black cumin seed, and borage were verified with cryo-SEM, which showed the advantage nanofibers have over microfibers as carriers for low-viscosity oils. The skin tests expressed the usability and the enhanced oil delivery performance for electrospun patches. We demonstrate that through the material nano- and microstructure, commercially available polymers such as PVB have great potential to be deployed as a biomaterial in medical applications, such as topical treatments for chronic skin conditions.

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Section: Introductionmentioning

confidence: 99%

Nano- and Microfiber PVB Patches as Natural Oil Carriers for Atopic Skin Treatment

Krysiak

Kaniuk

Metwally

et al. 2020

ACS Appl. Bio Mater.

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“…In a recent study, our group published data which showed that polymers based on poly(vinyl butyral-co-vinyl alcohol-co-vinyl acetate), functionalized oligolactone-based networks and urethane methacrylates have excellent cytocompatible properties [21].…”

Section: Discussionmentioning

confidence: 99%

“…However, up to now, there is no grafting material available that unites osteoinductive and -conductive characteristics with local antimicrobial activity and satisfactory physical stability. In this regard, our group already published data showing that polymers of poly(vinyl butyral-co-vinyl alcohol-co-vinyl acetate, urethane methacrylate or functionalized oligolactones are of promising characteristics [21].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial photodynamic active biomaterials for periodontal regeneration

et al. 2018

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Both materials show excellent mechanical and cytocompatible properties. In addition, irradiation with 652nm induced significant bacterial suppression. The manufactured biomaterials might enable a more efficient cure of periodontal bone lesions. Due to the mechanical properties functional stability might be increased. Further, the materials are antimicrobial upon illumination with light that enables a trans-mucosal eradication of residual pathogens.

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“…The proliferation of the cells was interpreted by changes of dsDNA quantity. The qualitative analysis of cell viability was performed by using Live/Dead staining according to reference at the third and seventh day. By this method, fluorescein diacetate stained viable cells green, whereas ethidium bromide stained the cell nuclei of dead cells in red.…”

Section: Methodsmentioning

confidence: 99%

Effects of Sodium Hydroxide, Sodium Hypochlorite, and Gaseous Hydrogen Peroxide on the Natural Properties of Cancellous Bone

Li²,

Huang³

et al. 2013

Artificial Organs

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Processed xenegeneic cancellous bone represents an alternative to bone autograft. In order to observe the effects of present prion inactivation treatments on the natural properties of xenogeneic cancellous bones, we treated bovine bone granules with sodium hydroxide (NaOH), sodium hypochlorite (NaOCl), and gaseous hydrogen peroxide (gH2 O2 ) respectively in this study. The microstructure, composition, and mineral content of the granules were evaluated by scanning electron micrograph, energy dispersive X-ray spectroscopy, ash analysis, and micro-computed tomography. The biomechanical property was analyzed by a materials testing machine. The cytocompatibility was evaluated by using a mouse fibroblast cell line (3T3). The microstructure, organic content, and mechanical strength were dramatically altered at the surface of bone in both NaOH- and NaOCl-treated groups, but not in the gH2 O2 -treated group. Compared with the gH2 O2 -treated group, attachment and proliferation of 3T3 were reduced in either NaOH- or NaOCl-treated groups. As the consequence, gH2 O2 treatment may be a useful approach of disinfection for the preparation of natural cancellous bone with well-preserved structural, mechanical, and biological properties.

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Cytocompatibility of polymer-based periodontal bone substitutes in gingival fibroblast and MC3T3 osteoblast cell cultures

Cited by 18 publications

References 62 publications

Nano- and Microfiber PVB Patches as Natural Oil Carriers for Atopic Skin Treatment

Nano- and Microfiber PVB Patches as Natural Oil Carriers for Atopic Skin Treatment

Antimicrobial photodynamic active biomaterials for periodontal regeneration

Effects of Sodium Hydroxide, Sodium Hypochlorite, and Gaseous Hydrogen Peroxide on the Natural Properties of Cancellous Bone

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