Cytogenetic and molecular study of two human neuroblastoma cell lines

Donti, Emilio; Longo, Letizia; Tonini, Gian Paolo; Verdona, Giovanna; Melodia, A.; Lanino, Edoardo; Cornaglia-Ferraris, P.

doi:10.1016/0165-4608(88)90188-4

Cited by 35 publications

(14 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Brodeur et al (1981) have localized the most frequently affected region to lp32-pter, and Kaneko et al (1985) reported frequent deletions in lp34-pter. More recently we and other groups have mapped the region of consistent deletions and/or translocations even more distal, i.e., to the lp36 band (Donti et al, 1988;Martinsson et al, 1989, unpublished data).…”

Section: Introductionmentioning

confidence: 90%

Neuroblastoma consensus deletion maps to 1p36.1–2

Weith

Martinsson²,

Cziepluch

et al. 1989

Genes Chromosomes & Cancer

162

View full text Add to dashboard Cite

At least 70% of human neuroblastomas display cytogenetically visible aberrations in the short arm of chromosome 1. We have used a panel of probes detecting polymorphic DNA loci, most of which were derived from a library of microdissected distal 1p chromosome fragments, to compare the hybridization pattern of DNA on nine different tumors and the corresponding normal tissue. In eight of the neuroblastomas allelic loss was observed with at least two probes. The deletions were of different size. Since a consensus deletion in all eight tumors included the segment 1p36.1-2, we conclude that genetic information related to neuroblastoma tumorigenesis is located within this approximately 10 megabase segment. Previous studies have revealed the amplification of MYCN in neuroblastomas. Our study did not provide evidence for a correlation between MYCN amplification and the 1p deletion, suggesting that the two genetic alterations result from molecular mechanisms that are not directly related to each other.

show abstract

Section: Introductionmentioning

confidence: 90%

Neuroblastoma consensus deletion maps to 1p36.1–2

Weith

Martinsson²,

Cziepluch

et al. 1989

Genes Chromosomes & Cancer

162

View full text Add to dashboard Cite

show abstract

“…These data emphasize the close relationship between NB and monosomy of the short arm of chromosome 1, supporting the hypothesis that a gene, or genes, capable of controlling the proliferation of neuroblasts (antioncogene) is, or are, present on this chromosomal segment (28). A duplication of the p21-p24 segment of the chromosome 2 short arm, involving the N-myc oncogene locus (2p23-24) (20), was observed from the 4th passage onward. The derivative chromosome generated by the t( 1 1,17)(q 13,qll) unbalanced translocation was simultaneously present with both members of the 17 pair and 1 single chromosome 11 in the diploid karyotype.…”

Section: Discussionmentioning

confidence: 99%

“…Some cytogenetic features appear to be peculiar for NB (20). The partial monosomy of chromosome 1, which is present in about 80% of fresh tumors and 65% of cell lines (27), was one of the more stable and initial anomalies, and very likely occurred in vivo.…”

Section: Discussionmentioning

confidence: 99%

“…We have analyzed more than 100 primary and metastatic NB and established new human NB cell lines (15,20). GI-ME-N derives from the BM of a child with stage IV NB.…”

Section: Discussionmentioning

confidence: 99%

“…Electrophoresis of 20 pg proteins obtained as described above was carried out onto 7.5 or 10% acrylamide slab gels using a Mini-Protean Dual Slab cell (BioRad Laboratories, Richmond, CA) at 120 V for 1 h, as detailed elsewhere (9) Chromosome preparations. The methods for chromosome analysis (20) and GTG-banding (15) have been described in detail.…”

Section: Patient Me Was a 2-yr-old Girl Diagnosed In December 1984mentioning

confidence: 99%

See 2 more Smart Citations

A New Human Highly Tumorigenic Neuroblastoma Cell Line with Undetectable Expression of N-myc

et al. 1990

View full text Add to dashboard Cite

ABSTRACT. A peculiar human cell line (GI-ME-N) derived from the metastatic bone marrow of a Zyr-old patient with stage IV neuroblastoma (NB) was extensively characterized. Cell-type-specific markers, tumorigenicity in nude mice, morphology, cytogenetics, and amplification/ expression of the N-myc gene were evaluated. All metaphases presented the typical l p deletion. Surface markers specific for NB cells, vimentin, and neurofilament proteins were all clearly detectable with immunofluorescence and/ or western blot procedures. Moreover, it was found that GI-ME-N cells did not express N-myc oncogene or HLA class 1 antigens, and were not classified as peripheral neuroectodermal tumor cells. However, extremely short latency and survival times, comparable to peripheral neuroectodermal tumor cells, were observed in nude mice grafted with GI-ME-N. In addition, no correlations were. observed in tumorigenicity of N-myc amplified (IMR32) versus unamplified (SK-N-SH GI-ME-N) human NB cell lines in nude mice. We conclude that N-myc amplification/ expression do not correlate with the aggressiveness of human NB in athymic animals, which is not always explained by the peripheral neuroectodermal tumor cell nature of the malignant cells, either. (Pediatr Res 27: 1-6, 1990) Abbreviations NB, neuroblastoma GI-ME-N, Gaslini Institute, patient initials, neuroblastoma PNET, peripheral neuroectodermal tumor BM, bone marrow s.c., subcutaneous Neuroblastoma, a neoplasm of the sympathetic nervous system, is the most common extracranial solid tumor arising in children (1, 2). In contrast to other pediatric tumors, the prognosis of NB patients has not significantly improved in the last 10 yr. With conventional chemotherapy, including local irradi-

show abstract