Cytogenetic assessment of Haworthia using flow cytometry and fluorescence in situ hybridization

Yesmin, Reshma; Mazharul, Islam Md; Deepo, Deen Mohammad; Kim, Hong Yul; Kim, Chang Kil; Lim, Ki Byung

doi:10.1007/s13580-021-00353-y

Cited by 2 publications

(2 citation statements)

References 44 publications

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“…Flow cytometry, as a widely used tool to establish nuclear DNA content in plants, is considered an effective tool during a mutant analysis to compare control and untreated plants with mutants (Xu et al, 2010;Lertsutthichawan et al, 2017;Yesmin et al, 2022;Deepo et al, 2023). In the present study, flow cytometry was used successfully to confirm the differences between succulent cultivar mutants and their respective control plants, as shown in histograms in Fig.…”

Section: Flow Cytometrymentioning

confidence: 61%

Phenotypic Evaluation and Nuclear DNA Content Analysis of Oryzalin-Induced Echeveria Mutant Cultivars

Cabahug-Braza,

Tran,

Lim

et al. 2023

HST

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Succulent plants are in high demand in the floriculture industry owing to their peculiar leaf arrangement, water-efficient characteristics, and wide color variation. In particular, the succulent genus Echeveria is preferred by many plant collectors and hobbyists. Therefore, continuous breeding programs for these succulents are underway to produce novel cultivars with new features, leading to higher market prices. As opposed to conventional breeding, chemical mutagenesis can achieve this goal. The use of oryzalin, an anti-microtubule drug, is effective to induce plant mutations and less hazardous to human health. Thus, in earlier work, we explored the use of oryzalin to induce mutant Echeveria cultivars, specifically the 'Brave', 'Momotarou', and 'Sistar'. Briefly, different concentrations (0.2%, 0.4%, 0.6%, 0.8%, and 1.0%) and dipping durations (3, 6, 9, and 12 h) of oryzalin were employed for chemical mutagenesis. Oryzalin produced Echeveria mutants with fewer, larger, darker, and thicker leaves compared to control plants. A stomatal analysis revealed that the mutants exhibited larger but fewer stomata and a lower epidermal cell density, consistent with polyploidization. Future studies involving chromosome counting and flow cytometry of putative oryzalin-induced mutants and their next generations are warranted to identify whether these alternations are reversible. Our findings will be useful for the mutation breeding of closely related ornamentals or other potted plants and development of new cultivars with novel traits.

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Section: Flow Cytometrymentioning

confidence: 61%

Phenotypic Evaluation and Nuclear DNA Content Analysis of Oryzalin-Induced Echeveria Mutant Cultivars

Cabahug-Braza,

Tran,

Lim

et al. 2023

HST

Self Cite

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“…In order to increase the amount of important plant metabolites, different methods are used such as elicitation and polyploidy induction (Thakur et al, 2019;Makowski et al, 2020). Most Aloe vera plants are diploid (Cavallini et al, 2012); As a result, the study of secondary metabolites was conducted only in diploid plants (Gantait et al, 2014) and there is no record in tetraploid plants (Pan et al, 2007;Zhang et al, 2007;Bano & Sharma 2020;Yesmin et al, 2021). Ploidy level has an effect on quantity of secondary metabolites.…”

Section: Introductionmentioning

confidence: 99%

Effects of methyl jasmonate, salicylic acid and phenylalanine on aloe emodin and aloin in diploid and tetraploid Aloe barbadensis

Shafighi

Moieni²,

Monfared³

2022

Int. j. hortic. sci.

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Aloe vera is one of the most famous medicinal plants. Aloin and aloe emodin are the most important active compounds in this plant. The purpose of this research was the comparison of aloin and aloe emodin production after the elicitation by methyl jasmonate, salicylic acid, and phenylalanine in diploid and tetraploid Aloe vera plants in greenhouse conditions. The plants were treated with the concentrations of 25, 50, and 100 µM. The amounts of aloin and aloe emodin were determined 24 and 48 hours after application of the treatment. HPLC analysis showed that the leaves of the control diploid plants (without applying elicitors) had more aloin (1.20 fold) and aloe emodin (1.14 fold) than the control tetraploid plants. The maximum concentration of aloin (1.15 ± 0.07 µg mg-1 dry weight) was obtained after the elicitation by 25 µM methyl jasmonate, 24 hours after treatment, in diploid plants) 6.36 fold compared to the control (0.18 µg mg-1 dry weight (. In addition, the maximum concentration of aloe emodin (0.28 µg mg-1 dry weight) was obtained after the elicitation by 25 µM salicylic acid, 24 hours after treatment, in diploid plants) 6.18 fold compared to the control (0.04 µg mg-1 dry weight)). The long-term effect of three studied elicitors (after 240 days) on plant health and survival was also studied. This investigation showed that only methyl jasmonate at a concentration of 100 µM was resulted in the death of Aloe vera plants.

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Cytogenetic assessment of Haworthia using flow cytometry and fluorescence in situ hybridization

Cited by 2 publications

References 44 publications

Phenotypic Evaluation and Nuclear DNA Content Analysis of Oryzalin-Induced Echeveria Mutant Cultivars

Phenotypic Evaluation and Nuclear DNA Content Analysis of Oryzalin-Induced Echeveria Mutant Cultivars

Effects of methyl jasmonate, salicylic acid and phenylalanine on aloe emodin and aloin in diploid and tetraploid Aloe barbadensis

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